Journal: Advanced Science

Article Title: CCR7 Mediated Mimetic Dendritic Cell Vaccine Homing in Lymph Node for Head and Neck Squamous Cell Carcinoma Therapy

doi: 10.1002/advs.202207017

Figure Lengend Snippet: Hy‐M‐Exo targeting lymph nodes. C3H mice were injected with DiR‐labeled nanovesicles. A) Fluorescence of the injection site was observed with IVIS imaging at 1, 3, 6, 12, 24, 36, and 48 h post‐injection. B) Total radiant efficiency of mice at different time post‐injection. Data were means ± standard error of mean deviation (SEM) ( n = 4). C) Popliteal and inguinal LNs were excised and analyzed with IVIS imaging at 12 and 24 h post‐injection. D) Popliteal LNs were excised, cryostat‐sectioned, stained, and observed under CLSM at 6 h post‐injection.

Article Snippet: Afterward, mice were imaged under IVIS Lumina X5 system (PerkinElmer, Waltham, USA).

Techniques: Injection, Labeling, Fluorescence, Imaging, Staining

Journal: AAPS PharmSciTech

Article Title: AddaVax Formulated with PolyI:C as a Potential Adjuvant of MDCK-based Influenza Vaccine Enhances Local, Cellular, and Antibody Protective Immune Response in Mice

doi: 10.1208/s12249-021-02145-0

Figure Lengend Snippet: Fluorescence imaging after administration of H3 combined with adjuvant in mice. Cy5.5-H3 alone or with AP, PolyI:C, and AddaVax were injected into BALB/c mice (100 µL/mouse), and fluorescence images were acquired using PE IVIS Lumina XRMS. Real-time monitoring of H3 antigen persistence at the injection sites or migration by in vivo fluorescence imaging

Article Snippet: The mice were anesthetized with 4% isoflurane exposure; then, 670 nm irradiation was implemented to the whole mouse for fluorescence images using PE IVIS Lumina XRMS (PerkinElmer, USA).

Techniques: Fluorescence, Imaging, Injection, Migration, In Vivo

Journal: Journal of Lipid Research

Article Title: Intravital lipid droplet labeling and imaging reveals the phenotypes and functions of individual macrophages in vivo

doi: 10.1016/j.jlr.2022.100207

Figure Lengend Snippet: Characterization of dye-loaded PLGA NPs in vivo. A: DiD/BODIPY-loaded PLGA NPs were designed and administered into in vitro-cultured macrophages. LDs (green color, BODIPY), DiD (red color), nuclei (blue color, Hoechst 33342); the scale bar represents 25 μm. B: Experimental setup. LPS was injected into the peritoneal cavity of mice to prime inflammation. On day 2 after LPS injection, DiD-loaded PLGA NPs were intravenously injected into the tail vein of mice. On day 3 after LPS injection, organs were harvested for analysis. C: Representative IVIS images of organs from normal mice, normal mice injected with NPs, and LPS-challenged mice injected with DiD-loaded PLGA NPs. D: A flow cytometer was used to assess the macrophage purity of DiD+-gated BM cells in normal and LPS-challenged mice after DiD-loaded PLGA NP injection. E: IF of half bone was used to identify the cell type of DiD+ BM cells from normal and LPS-challenged mice after DiD-loaded PLGA NP injection; F4/80 (green color), DiD (red color), nuclei (blue color, Hoechst 33342); the scale bar represents 100 μm.

Article Snippet: Fluorescence imaging was performed using the In Vivo Imaging System (IVIS®) Lumina S5™ (PerkinElmer).

Techniques: In Vivo, In Vitro, Cell Culture, Injection, Flow Cytometry