Journal: International Journal of Molecular Sciences

Article Title: Deletion of Notch3 Impairs Contractility of Renal Resistance Vessels Due to Deficient Ca 2+ Entry

doi: 10.3390/ijms232416068

Figure Lengend Snippet: Comparative real-time PCR mRNA expression of 47 genes related to contractile function in the afferent arterioles from Notch3 −/− and wild-type littermates. Note that the expression of Cacna1h coding the α 1H subunit of the T-type Ca 2+ channel (Ca v 3.2) gene was strongly downregulated in Notch3 −/− ( p < 0.001 vs. wild-type). In contrast, Cacna1c coding the α 1C subunit of the L-type Ca 2+ channel was similarly expressed in the two strains (No. 26 on the list).

Article Snippet: 11 , IP3-receptor , Ip3r1 , Itpr1-Mm00439917_m1 , 0.68 ± 0.02 , 0.62 ± 0.02 , −0.12 , 0.05.

Techniques: Real-time Polymerase Chain Reaction, Expressing

Journal: eLife

Article Title: 5-Hydroxymethylcytosine-mediated active demethylation is required for mammalian neuronal differentiation and function

doi: 10.7554/eLife.66973

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-Itpr1 (mouse monoclonal) , Origene , Origene: TA326547 , (1:500).

Techniques: Generated, Recombinant, Construct, Sequencing, Methylation Sequencing

Journal: eLife

Article Title: 5-Hydroxymethylcytosine-mediated active demethylation is required for mammalian neuronal differentiation and function

doi: 10.7554/eLife.66973

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-Itpr1 (mouse monoclonal) , Origene , Origene: TA326547 , (1:500).

Techniques:

Journal: bioRxiv

Article Title: A PACAP-activated network for secretion requires coordination of Ca 2+ influx and Ca 2+ mobilization

doi: 10.1101/2024.01.03.574069

Figure Lengend Snippet: A . Quantitative PCR showed chromaffin cells express all 3 of the IP3 receptor isoforms, but only 2 RyR isoforms. Mean transcript levels of IP3R1 (0.05 ± 0.003) were approximately 4-fold higher than IP3R2 (0.01 ± 0.002) and almost 10-fold higher than IP3R3 (0.005 ± 0.004). Expression levels were compared to β-actin. B . IP3R and RyR isoforms showed a similar pattern of expression in WT and PLCε KO cells. C and D . Western blot analysis comparing the protein expression levels of IP3R1 in WT and PLCε KO cells. The intensity of IP3R1 bands (WT vs KO) were quantified relative to β-actin and were not significantly different (Student’s t-test; p = 0.6).

Article Snippet: The scrambled shRNA and IP3R1 shRNA plasmids were purchased from OriGene; the 29mer sequence of scrambled shRNA (Cat# TR30015) is 5’-GCACTACCAGAGCTAACTCAGATAGTACT-3’, the 29mer sequence of IP3R1 (also called Itpr1) shRNA (Cat# TF517036) is 5’-TCAGCACCTTAGGCTTGGTTG-ATGACCGT-3’, and the transfected cells were identified based on their red fluorescence encoded by shRNA vectors.

Techniques: Real-time Polymerase Chain Reaction, Expressing, Western Blot

Journal: bioRxiv

Article Title: A PACAP-activated network for secretion requires coordination of Ca 2+ influx and Ca 2+ mobilization

doi: 10.1101/2024.01.03.574069

Figure Lengend Snippet: A . Confocal images showing immunostaining for the ER marker KDEL (green) and IP3R1 (red) in a chromaffin cell. Nuclei labeled with DAPI (blue). B and C . KDEL and IP3R1 intensities along the lines were similar. D . Manders’ coefficient analysis for the colocalization of KDEL and IP3R1 in n = 25 cells. Coefficient for IP3R1 overlap with KDEL = 0.7 ± 0.02; coefficient for KDEL overlap with IP3R1 = 0.8 ± 0.02.

Article Snippet: The scrambled shRNA and IP3R1 shRNA plasmids were purchased from OriGene; the 29mer sequence of scrambled shRNA (Cat# TR30015) is 5’-GCACTACCAGAGCTAACTCAGATAGTACT-3’, the 29mer sequence of IP3R1 (also called Itpr1) shRNA (Cat# TF517036) is 5’-TCAGCACCTTAGGCTTGGTTG-ATGACCGT-3’, and the transfected cells were identified based on their red fluorescence encoded by shRNA vectors.

Techniques: Immunostaining, Marker, Labeling

Journal: bioRxiv

Article Title: A PACAP-activated network for secretion requires coordination of Ca 2+ influx and Ca 2+ mobilization

doi: 10.1101/2024.01.03.574069

Figure Lengend Snippet: A . Western blot showing IP3R1 immunoreactive bands in control (scrambled shRNA) and IP3R1 KD samples. B . Intensity IP3R1 was normalized to β-actin expression. Relative intensities of bands (means ± SEM) of scrambled = 1 ± 0.007 and KD = 0.4 ± 0.06. Differences were statistically significant (Student’s t-test; p = 0.01). C . Representative traces of PACAP-stimulated Cal-520 fluorescence changes in control (untransfected) cells, scrambled shRNA, IP3R1 KD cells. D . Scatter plot of total spike area (n=49 control; n=17 scrambled shRNA; n=29 IP3R1 shRNA) represented as medians ± interquartile range. A Kruskal-Wallis test was used to assess statistical significance of differences. Control vs IP3R1 KD, p = 2×10 -9 ; IP3R1 KD vs scrambled, p = 0.0003. E . Scatter plot of max amplitude are represented as medians ± interquartile range. A Kruskal-Wallis test was used to assess statistical significance of differences. Control vs IP3R1 KD, p = 7×10 -9 ; IP3R1 KD vs scrambled, p = 3×10 -5 . F . FFN511 release was measured in cells stimulated by PACAP. Responses were reported as fusion events per unit area. n=12 cells for scrambled shRNA and n=13 cells for IP3R1 shRNA. Differences between groups were statistically significant (Mann-Whitney, p = 7×10 -7 ).

Article Snippet: The scrambled shRNA and IP3R1 shRNA plasmids were purchased from OriGene; the 29mer sequence of scrambled shRNA (Cat# TR30015) is 5’-GCACTACCAGAGCTAACTCAGATAGTACT-3’, the 29mer sequence of IP3R1 (also called Itpr1) shRNA (Cat# TF517036) is 5’-TCAGCACCTTAGGCTTGGTTG-ATGACCGT-3’, and the transfected cells were identified based on their red fluorescence encoded by shRNA vectors.

Techniques: Western Blot, Control, shRNA, Expressing, Fluorescence, MANN-WHITNEY

Journal: bioRxiv

Article Title: A PACAP-activated network for secretion requires coordination of Ca 2+ influx and Ca 2+ mobilization

doi: 10.1101/2024.01.03.574069

Figure Lengend Snippet: A and B. Scatter plot of total spike area and maximum %ΔF/F of Cal520 fluorescence. Cells were stimulated by 1 μM DMPP. n=51 control (untransfected), n=12 for scrambled shRNA, n=27 for IP3R1 KD. Data are shown as medians ± interquartile range. Differences between groups were not statistically significant (Kruskal-Wallis; p > 0.05).

Article Snippet: The scrambled shRNA and IP3R1 shRNA plasmids were purchased from OriGene; the 29mer sequence of scrambled shRNA (Cat# TR30015) is 5’-GCACTACCAGAGCTAACTCAGATAGTACT-3’, the 29mer sequence of IP3R1 (also called Itpr1) shRNA (Cat# TF517036) is 5’-TCAGCACCTTAGGCTTGGTTG-ATGACCGT-3’, and the transfected cells were identified based on their red fluorescence encoded by shRNA vectors.

Techniques: Fluorescence, Control, shRNA

Journal: Frontiers in Molecular Neuroscience

Article Title: Expression of genes encoding the calcium signalosome in cellular and transgenic models of Huntington's disease

doi: 10.3389/fnmol.2013.00042

Figure Lengend Snippet: List of genes and their annotations included in the RT-qPCR array .

Article Snippet: Itpr1 , inositol 1,4,5-trisphosphate receptor 1 , Mm00439907_m1.

Techniques: Binding Assay

Journal: Toxics

Article Title: Early Pregnancy Exposure to Ambient Air Pollution among Late-Onset Preeclamptic Cases Is Associated with Placental DNA Hypomethylation of Specific Genes and Slower Placental Maturation

doi: 10.3390/toxics9120338

Figure Lengend Snippet: Differentially methylated positions (ranked by q -value) between comparisons of combinations of preeclampsia (PE) status and ambient nitrogen oxides (NO x ) exposure group.

Article Snippet: cg04574034 , 17 , PRAC , PRAC small nuclear protein , 2.88 (2.03, 4.08) , 0.8 , 0.016.

Techniques: Methylation, Binding Assay