Journal: Endocrinology

Article Title: Autocrine and paracrine mechanisms of prostaglandin E₂ action on trophoblast/conceptus cells through the prostaglandin E₂ receptor (PTGER2) during implantation.

doi: 10.1210/en.2012-2271

Figure Lengend Snippet: Figure 5. PGE2 (100 nM) Induces Increased Adhesion of Primary Porcine Trophoblast Cells (A) and Human Trophoblast HTR-8/SVneo Cells (B) to ECM Fibronectin. Both types of cells were preincubated with either vehicle, PTGER2 antagonist (10 M AH609), 1 mM RGDS, or 1 mM RGES peptides, integrin (anti-ITGAVB3) antibody, or 50 g IgG for 30 minutes and then seeded onto fibronectin-coated plates in the presence of vehicle or 100 nM PGE2 for 1 hour (HTR-8/SVneo cells) or 3 hours (primary porcine trophoblast cells). Cell adhesion was calculated as n-fold increase relative to the control. Each treatment was carried out in triplicate, and each bar represents the mean SEM of at least 5 independent experiments. Bars with different letters (a–c) indicate significant differences (P .05).

Article Snippet: In main experiment, isolated porcine trophoblast cells and HTR-8/SVneo cells were incubated in phenol red-free medium 199 containing 1% NCS and RPMI containing 1% FBS, respectively, in the presence/absence of 1 mM arg-gly-asp-ser (RGDS; ITGAVB3-directed tetrapeptide arg-gly-asp-ser), 1 mM arg-glyglu-ser (RGES) tetrapeptide, 10 M AH6809, 50 g anti-ITGAVB3 antibody (integrin v 3; SC-7312, Santa Cruz Biotechnology, Inc. Santa Cruz, California) for 30 minutes prior to the addition of vehicle or 100 nM PGE2.

Techniques: Control