Journal: Biomicrofluidics

Article Title: Extracellular matrix gene expression profiling using microfluidics for colorectal carcinoma stratification

doi: 10.1063/1.4966245

Figure Lengend Snippet: Candidate cancer-related genes that may play roles in regulating the composition of the ECM and be involved in the progression and development of cancer.

Article Snippet: Integrin, alpha 8 , ITGA8 , 10p13 , Hs00233321_m1 , 89 , 30.

Techniques: Binding Assay

Journal: Biomicrofluidics

Article Title: Extracellular matrix gene expression profiling using microfluidics for colorectal carcinoma stratification

doi: 10.1063/1.4966245

Figure Lengend Snippet: The dysregulated gene panel (Figure ) was analysed as a combined cohort. Values shown in the table show expression fold change between normal and cancerous samples and associated p-values (statistical significance).

Article Snippet: Integrin, alpha 8 , ITGA8 , 10p13 , Hs00233321_m1 , 89 , 30.

Techniques: Expressing

Journal: Biomicrofluidics

Article Title: Extracellular matrix gene expression profiling using microfluidics for colorectal carcinoma stratification

doi: 10.1063/1.4966245

Figure Lengend Snippet: Genes with dysregulated expression from Figure are shown. Statistically significant correlated (co-expressed) genes are highlighted in bold.

Article Snippet: Integrin, alpha 8 , ITGA8 , 10p13 , Hs00233321_m1 , 89 , 30.

Techniques: Expressing

Journal: Advanced Science

Article Title: Targeting Itga8 Mitigates Neurogenic Bladder Fibrosis Driven by Trem2⁺ Macrophage‐Derived Fn1 via FAK/RhoA/ROCK Signaling

doi: 10.1002/advs.202510631

Figure Lengend Snippet: Itga8 + fibroblasts represent a critical subpopulation driving neurogenic bladder fibrosis. A) UMAP plot of subclustered fibroblasts. B) Dot plot of markers for each subset. C) Frequency of each cluster at different time points. D) Representative immunofluorescence images and quantitative analysis of Col1a2 (green), Itga8 (red), and DAPI (blue) expression in different groups, n = 3 per group. E) Representative Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment of the marker genes expressed in each subset of fibroblasts. F) Box plots of the collagen signature score for each fibroblast subset. G) UMAP plot of Acta2 . H) UMAP plot of Cthrc1 . I,J) Scatter plot demonstrating a significant positive correlation between Itga8 and (I) Acta2 or J) Cthrc1 . K) Dot plots demonstrated the expression dynamics of Acta2 , Cthrc1 , Itga8 , and Myl9 along pseudo‐time. L) The hdWGCNA dendrogram of seven identified modules. M) A bubble plot represented the scores of the seven modules in each subset. N) Networks of the representative genes from module 7. O) Enrichment analysis of each module. Fib. = Fibroblasts. Data represent mean ± SD. One‐way ANOVA was used for D. *** p < 0.001.

Article Snippet: The Col1a2 ‐CreERT; Itga8 fl/fl mice were generated by Cyagen Bioscience (Suzhou, China).

Techniques: Immunofluorescence, Expressing, Marker

Journal: Advanced Science

Article Title: Targeting Itga8 Mitigates Neurogenic Bladder Fibrosis Driven by Trem2⁺ Macrophage‐Derived Fn1 via FAK/RhoA/ROCK Signaling

doi: 10.1002/advs.202510631

Figure Lengend Snippet: Itga8 promotes fibroblast contraction and migration via the FAK and RhoA/ROCK signaling pathways. A) Representative images of the contraction assay in fibroblasts transduced with OE‐NC or OE‐Itga8 lentivirus. B) Quantification of cell contraction in (A), n = 4 per group. C) Representative images of the scratch assay in fibroblasts transduced with OE‐NC or OE‐Itga8 lentivirus. D) Statistical analysis of the wound area over time in (C), n = 3 per group. E) Representative images of immunofluorescence staining in fibroblasts transduced with OE‐NC or OE‐Itga8 lentivirus. F) Quantification of α‐SMA/F‐actin in (E), n = 3 per group. G) qRT‐PCR analysis of Acta2 mRNA expression, n = 3 per group. H) qRT‐PCR analysis of Cthrc1 mRNA expression, n = 3 per group. I) Representative images of the contraction assay in fibroblasts transduced with sh‐NC or sh‐Itga8 lentivirus. J) Quantification of cell contraction in (I), n = 6 per group. K) Representative images of the scratch assay in fibroblasts transduced with sh‐NC or sh‐Itga8 lentivirus. L) Statistical analysis of the wound area over time in (K), n = 3 per group. M) Representative images of immunofluorescence staining in fibroblasts transduced with sh‐NC or sh‐Itga8 lentivirus. N) Quantification of α‐SMA/F‐actin in (M), n = 3 per group. O) RNA sequencing of primary bladder fibroblasts transduced with sh‐Itga8 or sh‐NC. The heatmap shows the downregulation of fibroblast activation‐related genes in sh‐Itga8 transduced cells. P) Gene set enrichment analysis (GSEA) revealed downregulation of the pathway regulating the actin cytoskeleton in the sh‐Itga8 group. Q) Representative images of Western blots of Itga8 and downstream molecules. R) Representative immunofluorescence images of FAK. S) Quantification of FAK fluorescence intensity in (R). T) Western blot analysis of phosphorylated FAK (p‐FAK), total FAK, and RhoA in primary bladder fibroblasts transfected with either a kinase‐dead FAK mutant (K454R). U) Representative images and quantification of the contraction assay in fibroblasts transduced with FAK K454R mutant plasmid. V) Representative images and quantification of the scratch assay in fibroblasts transduced with FAK K454R mutant plasmid. W,X) Representative Western blot images of fibroblasts overexpressing Itga8 treated with the (W) FAK inhibitor PF‐573228 or the (X) Rho kinase inhibitor CCG‐1423. n.s. = not statistically significant; NPNT = nephronectin; PF = PF‐573228; CCG = CCG‐1423. Data represent mean ± SD. Unpaired two‐tailed t‐test was used for B, F, S, and U. One‐way ANOVA was used for G, H, J, and N. Two‐way ANOVA was used for D, L, and V. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The Col1a2 ‐CreERT; Itga8 fl/fl mice were generated by Cyagen Bioscience (Suzhou, China).

Techniques: Migration, Protein-Protein interactions, Contraction Assay, Transduction, Wound Healing Assay, Immunofluorescence, Staining, Quantitative RT-PCR, Expressing, RNA Sequencing, Activation Assay, Western Blot, Fluorescence, Transfection, Mutagenesis, Plasmid Preparation, Two Tailed Test

Journal: Advanced Science

Article Title: Targeting Itga8 Mitigates Neurogenic Bladder Fibrosis Driven by Trem2⁺ Macrophage‐Derived Fn1 via FAK/RhoA/ROCK Signaling

doi: 10.1002/advs.202510631

Figure Lengend Snippet: Trem2⁺ macrophages interact with fibroblasts via the Fn1‐Itga8 ligand‐receptor pairing. A) UMAP plot of subclustered macrophages. B) Dot plot of markers for each subset. C) Frequency of each cluster at different time points. D) Representative immunofluorescence images and quantitative analysis of Cd68 (red), Trem2 (yellow), and DAPI (blue) expression in different groups, n = 3 per group. E) Representative GO and KEGG enrichment of the marker genes expressed in each subset of macrophages. F) Box plots of the ECM score for each subset. G) Box plots of the collagen score for each subset. H) Box plots of the pro‐fibrotic score for each subset. I) Box plots of the anti‐fibrotic score for each subset. J) Bubble plot showing the primary ligand‐receptor pairs involved in the interaction between Trem2⁺ macrophages and Itga8⁺ fibroblasts. K) Representative western blot images of Itga8, Itgb1, Itga5, and Fn1. L) Representative images of the immunoprecipitation assay in fibroblasts. M) Representative images of the immunoprecipitation assay in 293T cells overexpressing Itga8. N) Dot plots demonstrated the expression dynamics of Fn1 and Trem2 along pseudo‐time. Mφ = Macrophage. Data represent mean ± SD. One‐way ANOVA was used for D. *** p < 0.001.

Article Snippet: The Col1a2 ‐CreERT; Itga8 fl/fl mice were generated by Cyagen Bioscience (Suzhou, China).

Techniques: Immunofluorescence, Expressing, Marker, Western Blot, Immunoprecipitation

Journal: Advanced Science

Article Title: Targeting Itga8 Mitigates Neurogenic Bladder Fibrosis Driven by Trem2⁺ Macrophage‐Derived Fn1 via FAK/RhoA/ROCK Signaling

doi: 10.1002/advs.202510631

Figure Lengend Snippet: Co‐culture experiments demonstrate that Fn1 derived from Trem2⁺ macrophages activates fibroblasts via Itga8. A) Schematic representation of the co‐culture experiment design. B) Flow cytometry confirms that IL‐17a induces the differentiation of BMDM into Trem2⁺ macrophages in vitro. C) Quantification of the percentage of Trem2 + macrophage in (B), n = 3 per group. D) Representative Western blot images of in vitro‐induced Trem2⁺ macrophages. E) ELISA analysis of Fn1 expression in the conditioned medium. F) Representative Western blot images of primary bladder fibroblasts treated with conditioned medium from IL‐17a‐induced BMDM. G,H) qRT‐PCR (G) and Western blot (H) analysis of Fn1 expression at the mRNA and protein levels following transfection with Fn1 siRNA. I) ELISA analysis of Fn1 expression in the culture supernatant following transfection with Fn1 siRNA. J) Representative Western blot images of fibroblasts treated with conditioned medium from IL‐17a‐induced BMDM transfected with either si‐NC or si‐Fn1. K) Representative images of the contraction assay in fibroblasts transduced with sh‐NC or sh‐Itga8 lentivirus and treated with the conditioned medium. L) Quantification of cell contraction in (K), n = 4 per group. M) Representative Western blot images of fibroblasts transduced with sh‐NC or sh‐Itga8 lentivirus and treated with conditioned medium from IL‐17a‐induced BMDM. N) Representative images of the scratch assay in fibroblasts transduced with sh‐NC or sh‐Itga8 lentivirus and treated with Fn1. O) Statistical analysis of the wound area over time in (N), n = 3 per group. P) Representative images of immunofluorescence staining in fibroblasts transduced with sh‐NC or sh‐Itga8 lentivirus and treated with Fn1. Q) Quantification of α‐SMA/F‐actin in (P), n = 3 per group. R,S) qRT‐PCR (R) and Western blot (S) analysis of Trem2 expression at the mRNA and protein levels following transfection with OE‐Trem2. T) Flow cytometry confirms that IL‐17a induces the differentiation of BMDM into Trem2⁺ macrophages in vitro. U) Quantification of the percentage of Trem2 + macrophage in (T), n = 3 per group. V) Representative Western blot images of fibroblasts transduced with sh‐NC or sh‐Itga8 lentivirus and treated with conditioned medium from Trem2‐overexpressing BMDM. Mφ = Macrophage. Data represent mean ± SD. Unpaired two‐tailed t‐test was used for C, E, G, I, R, and U. One‐way ANOVA was used for L, and Q. Two‐way ANOVA was used for O. * P < 0.05, ** P < 0.01, *** p < 0.001. Figure was created in BioRender. Wang, J. (2025) https://BioRender.com/kp68v7m .

Article Snippet: The Col1a2 ‐CreERT; Itga8 fl/fl mice were generated by Cyagen Bioscience (Suzhou, China).

Techniques: Co-Culture Assay, Derivative Assay, Flow Cytometry, In Vitro, Western Blot, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Transfection, Contraction Assay, Transduction, Wound Healing Assay, Immunofluorescence, Staining, Two Tailed Test

Journal: Advanced Science

Article Title: Targeting Itga8 Mitigates Neurogenic Bladder Fibrosis Driven by Trem2⁺ Macrophage‐Derived Fn1 via FAK/RhoA/ROCK Signaling

doi: 10.1002/advs.202510631

Figure Lengend Snippet: Knockdown of Itga8 improves urodynamic parameters and bladder fibrosis in rats. A) Schematic diagram of the animal experimental design. B) Representative urodynamic features during the acute and chronic phases of BPNI in the control, NB, NB + sh‐NC, and NB + sh‐Itga8 groups. The black arrow indicates a single voiding event. C) Bar graphs representing the maximum intravesical pressure, change in intravesical pressure, and voiding efficiency (calculated as voided volume/maximum bladder capacity) for each group, n = 6 per group. D) Representative images of Masson's trichrome staining for each group. E) Quantification of the ratio of smooth muscle to collagen in (E), n = 3 per group. F) Measurement of hydroxyproline content during the acute and chronic phase of BPNI, n = 3 per group. G,H) Representative western blot images (G) and quantitative analysis (H) of Itga8, FAK, RhoA, ROCK1, ROCK2, and Myl9. Data represent mean ± SD. One‐way ANOVA was used for C, E, F, and H. Compared with the control group, * p < 0.05, ** p < 0.01, *** p < 0.001. Compared with the sh‐NC group, # p < 0.05, ## p < 0.01, and ### p < 0.001. Figure was created in BioRender. Wang, J. (2025) https://BioRender.com/3xlbk8m .

Article Snippet: The Col1a2 ‐CreERT; Itga8 fl/fl mice were generated by Cyagen Bioscience (Suzhou, China).

Techniques: Knockdown, Control, Staining, Western Blot

Journal: Advanced Science

Article Title: Targeting Itga8 Mitigates Neurogenic Bladder Fibrosis Driven by Trem2⁺ Macrophage‐Derived Fn1 via FAK/RhoA/ROCK Signaling

doi: 10.1002/advs.202510631

Figure Lengend Snippet: Col1a2 ‐CreERT; Itga8 fl/fl mice demonstrates that Itga8 deletion delays fibrosis progression and protects bladder function. A) Schematic diagram of the animal experimental design. B) Representative immunofluorescence images of Col1a2 (green), Itga8 (red), and DAPI (blue) expression in different groups. C) Quantitative analysis of percentage of Itga8 + fibroblasts, n = 3 per group. D) Representative flow cytometry plots of Itga8 + fibroblasts in each group. E) Quantification of the percentage of Itga8 + fibroblasts in (D), n = 3 per group. F) Representative urodynamic profiles during the acute and chronic phases of BPNI in Col1a2 ‐CreERT⁺; Itga8 fl/fl mice and their littermate controls. The black arrow indicates a single voiding event. G,H) Bar graphs representing (G) delta intravesical pressure and (H) voiding efficiency (calculated as voided volume/maximum bladder capacity) for each group, n = 6 per group. I) Representative images of Masson's trichrome staining for each group. J) Quantification of the ratio of smooth muscle to collagen in (I), n = 3 per group. K) Measurement of hydroxyproline content during the acute and chronic phase of BPNI, n = 3 per group. L,M) Representative western blot images (L) and quantitative analysis (M) of isolated fibroblasts from the acute and chronic phases of BPNI in Col1a2 ‐CreERT⁺; Itga8 fl/fl mice and their littermate controls. Data represent mean ± SD. An unpaired two‐tailed t ‐test was used for C, G, H, K, and M. One‐way ANOVA was used for E and J. * p < 0.05, ** p < 0.01, *** p < 0.001. Figure was created in BioRender. Wang, J. (2025) https://BioRender.com/avquqdv .

Article Snippet: The Col1a2 ‐CreERT; Itga8 fl/fl mice were generated by Cyagen Bioscience (Suzhou, China).

Techniques: Immunofluorescence, Expressing, Flow Cytometry, Staining, Western Blot, Isolation, Two Tailed Test

Journal: Advanced Science

Article Title: Targeting Itga8 Mitigates Neurogenic Bladder Fibrosis Driven by Trem2⁺ Macrophage‐Derived Fn1 via FAK/RhoA/ROCK Signaling

doi: 10.1002/advs.202510631

Figure Lengend Snippet: Schematic diagram for the process of Itga8 + fibroblast activation in the context of neurogenic bladder fibrosis. In the normal bladder, fibroblasts and macrophages were in a state of quiescence. In contrast, neurogenic bladder was characterized by a substantial augmentation in the population of Itga8⁺ fibroblasts during the acute phase post‐injury. Moreover, fibroblast activation was orchestrated by Trem2⁺ macrophages, which secreted Fn1 to engage Itga8 on fibroblasts, thereby reinforcing the pro‐fibrotic communication between fibroblasts and macrophages. This, in turn, activated the FAK/RhoA/ROCK pathway and increased the expression of pro‐fibrotic genes through cytoskeletal remodeling, leading to fibroblast activation and ultimately to bladder fibrosis. Inhibition of Itga8 was expected to block the pathways mentioned above, delay fibrogenesis, and preserve bladder function integrity. Created in BioRender. Wang, J. (2025) https://BioRender.com/m2gslzz .

Article Snippet: The Col1a2 ‐CreERT; Itga8 fl/fl mice were generated by Cyagen Bioscience (Suzhou, China).

Techniques: Activation Assay, Expressing, Inhibition, Blocking Assay

Journal: Frontiers in Cellular Neuroscience

Article Title: Cochlear transcriptome analysis of an outbred mouse population (CFW)

doi: 10.3389/fncel.2023.1256619

Figure Lengend Snippet: Analysis and annotations of transcriptomics clusters using FR-Match. Comparison of transcriptomics clusters against five recently released snRNA and scRNA postnatal using the cluster-specific marker genes identified by NS-Forest.

Article Snippet: 60 , 8.2 , Cochlear fibrocyte 7 , Cochlear fibrocyte Itga8 Slit2 , Fibrocyte , Fibrocyte , Fibrocyte | Spiral ligament/cochlear ganglion , .

Techniques: Comparison, Marker, Membrane

Journal: Computational and Structural Biotechnology Journal

Article Title: Downregulation of ABLIM3 confers to the metastasis of neuroblastoma via regulating the cell adhesion molecules pathway

doi: 10.1016/j.csbj.2024.04.024

Figure Lengend Snippet: Downregulation of ABLIM3 promotes NB metastasis via downregulating the expression of ITGA3/ITGA8/KRT19. (A) The heatmap of the top 20 differentially expressed genes, as determined by the median expression levels of ABLIM3 in GSE49710 (including 498 NB samples). Upregulated genes are denoted in red, while downregulated genes are indicated in blue; (B) The Volcano plot displays the distribution of differentially expressed genes, with red indicating upregulation, green indicating downregulation, and black indicating genes that did not show significant changes in expression levels (C-D) Enrichment analysis of Gene Ontology (GO) terms, encompassing Biological Process (BP), Cellular Component (CC), and Molecular Function (MF) categories, as well as Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, were conducted for the differentially expressed genes; (E) The GSEA pathway analysis between ABLIM3 high expression and ABLIM3 low expression groups; (F, G) The mRNA expression levels of genes enriched in the Cell Adhesion Molecules (CAMs) pathway were assessed in SK-N-AS and SK-N-BE2 cells using RT-qPCR following transfection with ABLIM3 shRNAs (Ctrl shRNA and shRNA#2) for 24 h, *** P < 0.001, **** P < 0.0001 control shRNA-transfected cells versus ABLIM3 siRNA#2-transfected cells; (H) Western blot analysis was performed to detect the protein expression levels of ITGA3, ITGA8, and KRT19 in neuroblastoma cells (SK-N-AS and SK-N-BE2) following transfection with ABLIM3 shRNAs (Ctrl shRNA and shRNA#2) for 48 h.

Article Snippet: Then they were incubated at 4 °C overnight with specific antibodies: anti-ABLIM3 (1:1000, sc-398575, Santa Cruz Biotechnology, California, USA), ITGA3 (1:1000, A17502, Abclonal, Wuhan, China), ITGA8 (1:1000, A13056, Abclonal, Wuhan, China), KRT19 (1:1000, A0247, Abclonal, Wuhan, China), β-actin (1:5000, 66009–1-Ig, Proteintech, Wuhan, China) and anti-GADPH (1:1000, Proteintech, Wuhan, China,).

Techniques: Expressing, Quantitative RT-PCR, Transfection, shRNA, Control, Western Blot