Journal: American Journal of Translational Research

Article Title: Isoliquiritigenin attenuates LPS-induced AKI by suppression of inflammation involving NF-κB pathway

doi:

Figure Lengend Snippet: ISL ameliorated LPS-induced AKI renal dysfunction in mouse model. A. The formula of Isoliquiritigenin (ISL). B and C. LPS increased the CREA and BUN which represent serious kidney inflammation. ISL can decrease CREA and BUN to protect renal function. The level of CREA and BUN was measured by assay kit. D. Histological changes were assessed using HE and PAS staining. LPS treatment induced severe renal pathological lesions compared to normal control mice. In contrast, ISL treatment reduced the pathological features in murine kidney (magnification, ×400); *P < 0.05 vs. the LPS group, ***P < 0.001 vs. the LPS group, ###P < 0.001 vs. the Ctrl group.

Article Snippet: Chemicals and reagents Isoliquiritigenin was purchased from Med-ChemExpress, USA (10208, MCE, USA) ( ).

Techniques: Measured Assay, Staining, Control

Journal: Frontiers in Plant Science

Article Title: Lanthanum enhances biomass and bioactive metabolite production in Glycyrrhiza uralensis via coordinated gene regulation

doi: 10.3389/fpls.2025.1696545

Figure Lengend Snippet: Metabolomics analysis of Glycyrrhiza uralensis secondary metabolites. (A) Metabolomics study design; (B) Glycyrrhiza uralensis and its major secondary metabolites; (C) Chromatograms of six constituents in Glycyrrhiza uralensis with internal standard; (D) Contents of six main secondary metabolites of Glycyrrhiza uralensis (D1) : Glycyrrhizic acid; (D2) : Glycyrrhetinic acid; (D3) : Liquiritin; (D4) : Liquiritigenin; (D5) : Isoliquiritin (D6) : Isoliquiritigenin). Data are presented as mean ± SD (n = 3). Significant differences between W and La groups were determined by Student's t-test (** p < 0.01, *** p < 0.001).

Article Snippet: Certified reference standards of glycyrrhizic acid (purity ≥98%, Shanghai Yuanye), liquiritin (≥98%, Shanghai Yuanye), liquiritigenin (≥98%, Shanghai Yuanye), isoliquiritigenin (≥96%, Shanghai Yuanye), isoliquiritin (≥98%, Shanghai Yuanye), and glycyrrhetinic acid (≥98%, Shanghai Yuanye) were accurately weighed (0.00146 g, 0.00103 g, 0.00203 g, 0.00286 g, 0.00242 g, and 0.00160 g, respectively), dissolved in HPLC-grade methanol (Merck), and serially diluted to prepare stock solutions (1 mg/mL).

Techniques:

Journal: Frontiers in Plant Science

Article Title: Lanthanum enhances biomass and bioactive metabolite production in Glycyrrhiza uralensis via coordinated gene regulation

doi: 10.3389/fpls.2025.1696545

Figure Lengend Snippet: Integrated correlation network of bioactive metabolites, biosynthetic genes, and growth traits in Glycyrrhiza uralensis under La treatment. (A) Glycyrrhizic acid biosynthesis pathway; (B) Liquiritin, liquiritigenin, isoliquiritigenin, and isoliquiritin biosynthesis pathway; (C) Correlation analysis of bioactive metabolites and biosynthetic genes; (D) Correlation Analysis of Bioactive Metabolites and Growth Traits; (E) Co-occurrence network displaying the correlation values between physiological growth parameters, metabolites and differential genes. Size of the node indicates the degree of these variables. Solid lines indicate significant correlations ( p < 0.05), while dashed lines denote non-significant correlations ( p ≥ 0.05).

Article Snippet: Certified reference standards of glycyrrhizic acid (purity ≥98%, Shanghai Yuanye), liquiritin (≥98%, Shanghai Yuanye), liquiritigenin (≥98%, Shanghai Yuanye), isoliquiritigenin (≥96%, Shanghai Yuanye), isoliquiritin (≥98%, Shanghai Yuanye), and glycyrrhetinic acid (≥98%, Shanghai Yuanye) were accurately weighed (0.00146 g, 0.00103 g, 0.00203 g, 0.00286 g, 0.00242 g, and 0.00160 g, respectively), dissolved in HPLC-grade methanol (Merck), and serially diluted to prepare stock solutions (1 mg/mL).

Techniques:

Journal: Cancer Research

Article Title: Castration-Induced Downregulation of SPARC in Stromal Cells Drives Neuroendocrine Differentiation of Prostate Cancer

doi: 10.1158/0008-5472.can-21-0163

Figure Lengend Snippet: Figure 7. The tumor-GRP78/stroma–SPARC network in human NE prostate cancer. A, Representative immunofluorescence for GRP78 (red) in LNCaP tumor cells treated for 24 hours with enzalutamide (ENZA). Blue, DAPI. B, Quantification of staining in A, Experiment was repeated two times. C and D, Real-time PCR for b-catenin (Ctnbb1 gene; D) and miR29b1 (E) in LNCaP cells treated with enzalutamide for 8 or 24 hours, respectively. E, ELISA for IL6 in LNCaP cells treated for 24 hours with enzalutamide, alone or in coculture with human prostatic fibroblasts (the WPMY-1 cell line, indicated as Human Fibro). F, Real-time PCR for Sparc in human fibro cocultured or not with LNCaP cells (indicated in parentheses)andtreated with enzalutamide, isoliquiritigenin, or their combination.Cellswere analyzedafter 48 hours of culture. G, Western blot analysis for SPARC in human fibro cultured as in F. Vinculin was used as internal control. The Western blot was validated twice. H, Quantification of G. I, Real-time PCR for Cga or Eno2 on LNCaP cells cocultured or not with human fibro (indicated in brackets) and treated with enzalutamide, isoliquiritigenin, or their combination. Cells were analyzed after 48 hours of culture. F and I, Data are a pool of three independent experiments. In all panels, histograms depict mean SD of biological replicates (represented by dots). Two-tailed Student t test or one-way ANOVA followed by Tukey test: , P < 0.05; , P < 0.01; , P < 0.001; , P < 0.0001. J, Mutation burden for Hspa5 gene (encoding for GRP78) in human prostate cancer, obtained on cBioportal analyzing data from the Kumar data set (NEPC and CRPC; ref. 36), the SU2C/PCF Dream Team data set of CRPC (35), and the TCGA. K, Spearman correlation between transcript levels of Hspa5 and Akt1 or Cdk5 in the Beltran data set of NEPC (6).

Article Snippet: When indicated, IL6 (50 ng/mL, PeproTech), mAb to IL6-R (50 ng/mL; D7715A7 clone, for in vitro experiments purchased from eBioscience, as indicated in Supplementary Table S1), enzalutamide (10 mmol/L; SelleckChem, catalog no. S1250), isoliquiritigenin (25 mmol/L, SelleckChem, catalog no. S2404) or HA15 (10 mmol/L SelleckChem, catalog no. S8299) were added.

Techniques: Staining, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Western Blot, Cell Culture, Control, Two Tailed Test, Mutagenesis

Journal: Scientific Reports

Article Title: Sirtuins transduce STACs signals through steroid hormone receptors

doi: 10.1038/s41598-020-62162-0

Figure Lengend Snippet: Subclasses of natural STACs used in this study and examples of their dietary sources.

Article Snippet: 17β-oestradiol (E2/oestrogen), kaempferol, quercetin, naringenin, formononetin, fulvestrant (ICI 182,780) and analytical grade reagents were purchased from Sigma-Aldrich (Dorset, U.K.); isoliquiritigenin was purchased from Santa Cruz Biotechnology (Wembley, London, U.K.), and resveratrol was from Enzo Lifesciences (Exeter, U.K.).

Techniques:

Journal: Scientific Reports

Article Title: Sirtuins transduce STACs signals through steroid hormone receptors

doi: 10.1038/s41598-020-62162-0

Figure Lengend Snippet: Sir-2.1 transduces dSTACs through the worm steroid receptor DAF-12. Hep3B cells cotransfected with DAF-12 and its reporter gene lit- 1k-TK-luc and Sir-2.1, were treated (T) with DMSO control or with an exemplar dSTACs alone: isoflavone (daidzein), chalcone (isoliquiritigenin) and stilbene (resveratrol) or in combination with 1 µM Δ 4 -DA as indicated. Fold activation was calculated from the activity of lit- 1k-TK-luc. Data sets were plotted as means ± S.E.M, (n = 3). Student’s t -test for differences in reporter gene activation by DMSO or STACs and STACs/Δ 4 -DA treatment are extremely significant ( P ≤ 0.001).

Article Snippet: 17β-oestradiol (E2/oestrogen), kaempferol, quercetin, naringenin, formononetin, fulvestrant (ICI 182,780) and analytical grade reagents were purchased from Sigma-Aldrich (Dorset, U.K.); isoliquiritigenin was purchased from Santa Cruz Biotechnology (Wembley, London, U.K.), and resveratrol was from Enzo Lifesciences (Exeter, U.K.).

Techniques: Control, Activation Assay, Activity Assay

Journal: Scientific Reports

Article Title: Breast cancer prevention with liquiritigenin from licorice through the inhibition of aromatase and protein biosynthesis in high-risk women’s breast tissue

doi: 10.1038/s41598-023-34762-z

Figure Lengend Snippet: GI and the phytoestrogens from hops and licorice exhibit the most pronounced aromatase inhibition effect compared to the other tested agents. Aromatase supersomes were incubated with various concentrations of ( A ) GI, GG, GU, and hop extracts, ( B ) 8-PN, XH, 6-PN from hops, and ( C ) LigF, 8-PA, LicA, LigC from licorice for 40 min at 37 °C. Formation of a fluorescent metabolite was measured at Ex/Em of 409 nm/530 nm. Data represents mean ± SD of at least three independent measurements. Ketoconazole was used as the positive control.

Article Snippet: Isoliquiritigenin (LigC) and liquiritigenin (LigF) were acquired from ChromaDex (Irvine, CA).

Techniques: Inhibition, Incubation, Positive Control

Journal: Scientific Reports

Article Title: Breast cancer prevention with liquiritigenin from licorice through the inhibition of aromatase and protein biosynthesis in high-risk women’s breast tissue

doi: 10.1038/s41598-023-34762-z

Figure Lengend Snippet: Aromatase inhibition and estrogen receptor subtype (ERα, ERβ) binding affinities of compounds from hops and licorice species.

Article Snippet: Isoliquiritigenin (LigC) and liquiritigenin (LigF) were acquired from ChromaDex (Irvine, CA).

Techniques: Inhibition, Binding Assay

Journal: Scientific Reports

Article Title: Breast cancer prevention with liquiritigenin from licorice through the inhibition of aromatase and protein biosynthesis in high-risk women’s breast tissue

doi: 10.1038/s41598-023-34762-z

Figure Lengend Snippet: Phytoestrogens bind the active site of aromatase similar to letrozole, an established aromatase inhibitor. ( A ) letrozole (light blue), ( B ) 8-PN (orange), ( C ) 8-PA (light blue), and ( D ) LigF (dark purple) docked into aromatase (ribbon)-heme (fuchsia) complex in its most energetically favorable conformation.

Article Snippet: Isoliquiritigenin (LigC) and liquiritigenin (LigF) were acquired from ChromaDex (Irvine, CA).

Techniques:

Journal: Scientific Reports

Article Title: Breast cancer prevention with liquiritigenin from licorice through the inhibition of aromatase and protein biosynthesis in high-risk women’s breast tissue

doi: 10.1038/s41598-023-34762-z

Figure Lengend Snippet: Hops and GI suppress the expression of CYP19A1 mRNA in high-risk breast tissue microstructures. Fresh surgically removed unaffected contralateral breast tissue from postmenopausal women undergoing double mastectomy due to unilateral breast cancer were processed to make microstructures using collagenase I, before culturing and treating them for 24 h with ( A ) hops (5 µg/mL), 8-PN (10 µM) vs. ( B ) GI (5 µg/mL), LigF (5 µM), LicA (5 µM). Subsequently, RNA extraction and qPCR of CYP19A1 was performed. The expression of CYP19A1 mRNA was calculated relative to the housekeeping gene RPLP0 and normalized to DMSO control. Data represents mean ± SD of three independent observations ( p < 0.05).

Article Snippet: Isoliquiritigenin (LigC) and liquiritigenin (LigF) were acquired from ChromaDex (Irvine, CA).

Techniques: Expressing, RNA Extraction, Control

Journal: Scientific Reports

Article Title: Breast cancer prevention with liquiritigenin from licorice through the inhibition of aromatase and protein biosynthesis in high-risk women’s breast tissue

doi: 10.1038/s41598-023-34762-z

Figure Lengend Snippet: Liquiritigenin does not enhance uterine weight and suppresses E 2 -induced uterine growth. Female immature Sprague–Dawley rats (n = 6 per group) at 18 days of age were weaned and on day 19 of age received vehicle control, estradiol benzoate (1.6 µg/kg.day), LigF (50 mg/kg.day), LigF (150 mg/kg.day), and LigF (150 mg/kg.day) + estradiol benzoate (1.6 µg/kg.day) subcutaneously for 3 days. Animals were sacrificed on day 4 and their uterine weights were measured. The effects of LigF depicted in the accompanying picture of the animals’ uterine tissue are associated with LigF (150 mg/kg.day).

Article Snippet: Isoliquiritigenin (LigC) and liquiritigenin (LigF) were acquired from ChromaDex (Irvine, CA).

Techniques: Control

Journal: Scientific Reports

Article Title: Breast cancer prevention with liquiritigenin from licorice through the inhibition of aromatase and protein biosynthesis in high-risk women’s breast tissue

doi: 10.1038/s41598-023-34762-z

Figure Lengend Snippet: Transcriptomic analysis of high-risk breast tissue microstructures exposed to LigF suggests downregulation of protein synthesis. Breast tissue microstructures prepared from surgical contralateral unaffected breast of postmenopausal women with unilateral breast cancer were exposed to LigF (5 µM) for 24 h. RNA was extracted and total RNA sequencing was performed. Differential gene expression was evaluated using a patient-controlled approach. Significantly (adj P < 0.05) modulated genes were further analyzed by Enrichr to define modulated pathways and ontologies.

Article Snippet: Isoliquiritigenin (LigC) and liquiritigenin (LigF) were acquired from ChromaDex (Irvine, CA).

Techniques: RNA Sequencing, Gene Expression

Journal: Scientific Reports

Article Title: Breast cancer prevention with liquiritigenin from licorice through the inhibition of aromatase and protein biosynthesis in high-risk women’s breast tissue

doi: 10.1038/s41598-023-34762-z

Figure Lengend Snippet: LigF retards breast cancer cell proliferation. MCF-7 cells were treated every 48 h with various concentrations of LigF for 6 days and observed for 11 days. Cell proliferation was monitored using live cell imaging by IncuCyte. Data represents the mean of at least two independent observations ± SEM.

Article Snippet: Isoliquiritigenin (LigC) and liquiritigenin (LigF) were acquired from ChromaDex (Irvine, CA).

Techniques: Live Cell Imaging

Journal: Plants

Article Title: The (Bio)chemical Base of Flower Colour in Bidens ferulifolia

doi: 10.3390/plants11101289

Figure Lengend Snippet: HPLC chromatograms at 385 nm from incubation of enzyme preparations from Bidens petals in the presence of NADPH using butein (top) and isoliquiritigenin (bottom) as substrates. Peaks 1–4 are background peaks resulting from remnants of anthochlor and flavonoid glycosides left by the enzyme preparation. 5: okanin, 6: 3′-hydroxyisoliquiritigenin, 7: butein, 8: isoliquiritigenin. Please note: the numbers after the retention labels 1-8 are the retention times in seconds, with a decimal comma instead of a period.

Article Snippet: Isoliquiritigenin, butein, apigenin, luteolin, naringenin, eriodictyol, dihydrokaempferol, dihydroquercetin, marein, maritimein, peonidin and cyanidin were purchased from Extrasynthese (Genay, France).

Techniques: Incubation

Journal: Journal of Microbiology and Biotechnology

Article Title: Development of an Improved Menopausal Symptom-Alleviating Licorice ( Glycyrrhiza uralensis ) by Biotransformation Using Monascus albidulus

doi: 10.4014/jmb.1909.09037

Figure Lengend Snippet: Contents of liquiritigenin and isoliquiritigenin, and monacolin K production in licorice fermented with Monascus strains.

Article Snippet: Monacolin K, citrinin, liquiritin, liquiritigenin, trifluoroacetic acid, acetic acid, and p -nitrophenyl-β-D-glucopyranoside were purchased from Sigma-Aldrich Co. (USA), isoliquiritin and isoliquiritigenin from ChemFaces (China), and liquiritin apioside and isoliquiritin apioside from Chengdu Biopurify Phytochemicals Ltd. (China).

Techniques:

Journal: Journal of Microbiology and Biotechnology

Article Title: Development of an Improved Menopausal Symptom-Alleviating Licorice ( Glycyrrhiza uralensis ) by Biotransformation Using Monascus albidulus

doi: 10.4014/jmb.1909.09037

Figure Lengend Snippet: HPLC chromatograms of liquiritin ( A ), liquiritigenin ( B ), isoliquiritin ( C ), and isoliquiritigenin ( D ).

Article Snippet: Monacolin K, citrinin, liquiritin, liquiritigenin, trifluoroacetic acid, acetic acid, and p -nitrophenyl-β-D-glucopyranoside were purchased from Sigma-Aldrich Co. (USA), isoliquiritin and isoliquiritigenin from ChemFaces (China), and liquiritin apioside and isoliquiritin apioside from Chengdu Biopurify Phytochemicals Ltd. (China).

Techniques:

Journal: Journal of Microbiology and Biotechnology

Article Title: Development of an Improved Menopausal Symptom-Alleviating Licorice ( Glycyrrhiza uralensis ) by Biotransformation Using Monascus albidulus

doi: 10.4014/jmb.1909.09037

Figure Lengend Snippet: Bioconversion of liquiritin/liquiritin apioside and isoliquiritin/isoliquiritin apioside, β-glucosidase activity, and monacolin K production during licorice fermentation. ( A ) Bioconversion of liquiritin and liquiritin apioside to liquiritigenin; ( B ) bioconversion of isoliquiritin and isoliquiritin apioside to isoliquiritigenin; ( C ) β-glucosidase activity; ( D ) monacolin K production.

Article Snippet: Monacolin K, citrinin, liquiritin, liquiritigenin, trifluoroacetic acid, acetic acid, and p -nitrophenyl-β-D-glucopyranoside were purchased from Sigma-Aldrich Co. (USA), isoliquiritin and isoliquiritigenin from ChemFaces (China), and liquiritin apioside and isoliquiritin apioside from Chengdu Biopurify Phytochemicals Ltd. (China).

Techniques: Activity Assay

Journal: Journal of Microbiology and Biotechnology

Article Title: Development of an Improved Menopausal Symptom-Alleviating Licorice ( Glycyrrhiza uralensis ) by Biotransformation Using Monascus albidulus

doi: 10.4014/jmb.1909.09037

Figure Lengend Snippet: Chemical structures of liquiritin ( A ), isoliquiritin ( B ), liquiritin apioside ( C ), isoliquiritin apioside ( D ), liquiritigenin ( E ), and isoliquiritigenin ( F ).

Article Snippet: Monacolin K, citrinin, liquiritin, liquiritigenin, trifluoroacetic acid, acetic acid, and p -nitrophenyl-β-D-glucopyranoside were purchased from Sigma-Aldrich Co. (USA), isoliquiritin and isoliquiritigenin from ChemFaces (China), and liquiritin apioside and isoliquiritin apioside from Chengdu Biopurify Phytochemicals Ltd. (China).

Techniques: