Journal: Stem Cell Research & Therapy

Article Title: Human umbilical cord mesenchymal stem cell-derived exosomal miR-27b attenuates subretinal fibrosis via suppressing epithelial–mesenchymal transition by targeting HOXC6

doi: 10.1186/s13287-020-02064-0

Figure Lengend Snippet: hucMSC-Exo treatment had less fibrosis development in vivo. a Study design. b The RPE–Bruch membrane complex was disrupted, the normal arrangement of the inner nuclear layer was lost and neovascular hide beneath the subretinal space 7 days after the laser burn. Compared with the PBS group, the number of collagen fibers reduced (blue) in the group treated with hucMSC-derived exosomes 35 days after laser photocoagulation. c Choroidal neovascularization (CNV; dyed with isolectin B4) and fibrosis (dyed with collagen type I) were recorded 7, 21, and 35 days after laser burn in the PBS and hucMSC-Exo groups. d Mean volume of the CNV and fibrosis 7, 21, and 35 days after laser burn

Article Snippet: Then, the RPE–choroid complexes were washed, blocked with 5% goat serum albumin and 0.3% Triton X-100, and then incubated with FITC-labeled isolectin-B4 (IB4) (Vector Laboratories, 1:100) (for evaluating CNV) and collagen type I antibody (ab34710, Abcam, dilution 1:100) (for evaluating subretinal fibrosis) at 4 °C overnight.

Techniques: In Vivo, Derivative Assay

Journal: Frontiers in Cardiovascular Medicine

Article Title: Empagliflozin inhibits coronary microvascular dysfunction and reduces cardiac pericyte loss in db/db mice

doi: 10.3389/fcvm.2022.995216

Figure Lengend Snippet: Effects of empagliflozin treatment on the cardiac microvasculature of db/db mice. (A) Representative transverse images of myocardial vasculature showing capillaries (IB4+, red). (B) Statistical analysis of the density of capillaries in the transverse sections of myocardial tissue between the db/m group, db/db group, and db/db + EMPA group. (C) Representative longitudinal images of myocardial vasculature showing capillaries (IB4+, red). (D) Statistical analysis of the diameter of capillaries in the longitudinal sections of myocardial tissue between the db/m group, db/db group, and db/db + EMPA group. Data are presented as the means ± SEM. P -values are corrected for multiple comparisons. IB4, isolectin B4; HPF, high power field.

Article Snippet: After washing with PBS (2 h * 3), the heart tissues were incubated in 200 μL staining buffer with secondary antibody conjugated to Alexa Fluor 647 (AB32733, Invitrogen, Carlsbad, CA, United States, 1:200) and isolectin B4 conjugated to FITC (L2895, Merck Millipore, Darmstadt, Germany, 1:20) for 3 days at 37°C with gentle shaking.

Techniques:

Journal: Frontiers in Cardiovascular Medicine

Article Title: Empagliflozin inhibits coronary microvascular dysfunction and reduces cardiac pericyte loss in db/db mice

doi: 10.3389/fcvm.2022.995216

Figure Lengend Snippet: Effects of empagliflozin treatment on pericytes in the hearts of db/db mice. (A) Representative myocardial sections showing pericytes (NG2+, green), capillaries (IB4+, red) and nuclei (DAPI+, blue). (B) Statistical analysis of pericyte number/HPF between the three groups. (C) Statistical analysis of pericyte coverage between the three groups, pericyte coverage = (NG2 + area/IB4 + area)/HPF*100%. Data are presented as the means ± SEM. P -values are corrected for multiple comparisons. NG2, neuroglial cell 2 chondroitin sulfate proteoglycan; IB4, isolectin B4; DAPI, 4′,6-diamidino-2-phenylindole; HPF, high power field.

Article Snippet: After washing with PBS (2 h * 3), the heart tissues were incubated in 200 μL staining buffer with secondary antibody conjugated to Alexa Fluor 647 (AB32733, Invitrogen, Carlsbad, CA, United States, 1:200) and isolectin B4 conjugated to FITC (L2895, Merck Millipore, Darmstadt, Germany, 1:20) for 3 days at 37°C with gentle shaking.

Techniques: