Journal: International Journal of Ophthalmology

Article Title: Nintedanib induces apoptosis in human pterygium cells through the FGFR2-ERK signalling pathway

doi: 10.18240/ijo.2023.04.03

Figure Lengend Snippet: A: Phase contrast microscopy of primary human pterygium-derived cells; B: Phase contrast microscopy of passaged human pterygium-derived cells (Generation 8); C: The results showed that the purity of pterygium epithelial cells accounted for up to 74%. MUC1: transmembrane glycoprotein mucin 1; K10: Keratin 10.

Article Snippet: The cells were then incubated in the dark for 30min at 4°C with FITC-labeled MUC1 (559774, BD) and PE-labeled K10 (orb485164, Biorbyt) antibodies.

Techniques: Microscopy, Derivative Assay

Journal: Frontiers in bioscience (Landmark edition)

Article Title: Single-Cell Transcriptome Analysis of Small Cell Neuroendocrine Carcinoma of the Endometrium Reveals ISL1 as a Potential Biomarker for Diagnosis and Treatment.

doi: 10.31083/j.fbl2903100

Figure Lengend Snippet: Fig. 5. ISL1 modulates neuroactive pathway in subclusters of single cell RNA-seq data and The Cancer Genome Atlas (TCGA)

Article Snippet: After cell counting, the cells were incubated with ISL LIM Homeobox 1 (ISL1) monoclonal antibody (1:200, 15661-1-AP; Proteintech, Rosemont, IL, USA) at 4 °C in the dark for 60 min, followed by incubation for 30 min with a fluorescent secondary antibody (1:200, SA00013-2, CoraLite488-conjugated goat anti-rabbit IgG (H+L)).

Techniques: RNA Sequencing

Journal: Frontiers in bioscience (Landmark edition)

Article Title: Single-Cell Transcriptome Analysis of Small Cell Neuroendocrine Carcinoma of the Endometrium Reveals ISL1 as a Potential Biomarker for Diagnosis and Treatment.

doi: 10.31083/j.fbl2903100

Figure Lengend Snippet: Fig. 6. Flow cytometric sorting of neuroendocrine tumor cells to obtain ISL1 expression group, and explore the differences in cell function in vitro. (A) Flow cytometric sorting of neuroendocrine tumor cell line H446 according to the difference in the expression of

Article Snippet: After cell counting, the cells were incubated with ISL LIM Homeobox 1 (ISL1) monoclonal antibody (1:200, 15661-1-AP; Proteintech, Rosemont, IL, USA) at 4 °C in the dark for 60 min, followed by incubation for 30 min with a fluorescent secondary antibody (1:200, SA00013-2, CoraLite488-conjugated goat anti-rabbit IgG (H+L)).

Techniques: Expressing, Cell Function Assay, In Vitro

Journal: Stem Cells International

Article Title: Dexamethasone Provides Effective Immunosuppression for Improved Survival of Retinal Organoids after Epiretinal Transplantation

doi: 10.1155/2019/7148032

Figure Lengend Snippet: Primers.

Article Snippet: Antibodies against the following markers were used as primary antibodies for immunofluorescence microscopy at the dilutions indicated in parentheses: Ki67 (1 : 100, A11390, ABclonal, China), CHX10 (1 : 200, AB9016, Millipore, Germany), Brn3 (1 : 200, SC-6026X, Santa Cruz, USA), Islet1 (1 : 20, BM44446, Boster, China), HuD (1 : 100, SC-48421, Santa Cruz), RBPMS (1 : 100, ab152101, Abcam), β III-tubulin (1 : 200, ab7751, Abcam), neurofilament light polypeptide (NEFL; 1 : 100, A0257, ABclonal), microtubule-associated protein 2 (MAP2, 1 : 100, BM1243, Boster), vimentin (1 : 50, BM4029, Boster), glial fibrillary acidic protein (GFAP; 1 : 500, 3670S, Cell Signaling Technology, USA), CD68 (1 : 200, ab201340, Abcam), Iba1 (1 : 200, ab5076, Abcam), and SC121 (1 : 500, Y40410, Takara Bio).

Techniques:

Journal: Stem Cells International

Article Title: Dexamethasone Provides Effective Immunosuppression for Improved Survival of Retinal Organoids after Epiretinal Transplantation

doi: 10.1155/2019/7148032

Figure Lengend Snippet: Characteristics of retinal organoids after 30-45 days of induction. The frozen sections of retinal organoids were identified by immunofluorescence staining. (a, b) At this time, most cells of the organoids were differentiating towards RGCs; they were Islet1-, Brn3- and HuD-positive. (c) But some cells outside were still retinal progenitors; they were CHX10-positive. (d, e) And in suspension cultivation, little axons were developed.

Article Snippet: Antibodies against the following markers were used as primary antibodies for immunofluorescence microscopy at the dilutions indicated in parentheses: Ki67 (1 : 100, A11390, ABclonal, China), CHX10 (1 : 200, AB9016, Millipore, Germany), Brn3 (1 : 200, SC-6026X, Santa Cruz, USA), Islet1 (1 : 20, BM44446, Boster, China), HuD (1 : 100, SC-48421, Santa Cruz), RBPMS (1 : 100, ab152101, Abcam), β III-tubulin (1 : 200, ab7751, Abcam), neurofilament light polypeptide (NEFL; 1 : 100, A0257, ABclonal), microtubule-associated protein 2 (MAP2, 1 : 100, BM1243, Boster), vimentin (1 : 50, BM4029, Boster), glial fibrillary acidic protein (GFAP; 1 : 500, 3670S, Cell Signaling Technology, USA), CD68 (1 : 200, ab201340, Abcam), Iba1 (1 : 200, ab5076, Abcam), and SC121 (1 : 500, Y40410, Takara Bio).

Techniques: Immunofluorescence, Staining, Suspension

Journal: Stem Cells International

Article Title: Dexamethasone Provides Effective Immunosuppression for Improved Survival of Retinal Organoids after Epiretinal Transplantation

doi: 10.1155/2019/7148032

Figure Lengend Snippet: Comparisons of different marker expression profiles among the control, rapamycin- (RAP-) treated, and dexamethasone- (DEX-) treated groups. Two weeks after immunosuppressant treatment, (a) cells in the retinal organoids treated with RAP expressed higher levels of the retinal ganglion cell (RGC) markers Atoh7, Islet1, and Brn3b, the dendrite marker MAP2, the retinal progenitor marker PAX6 and CHX10, and photoreceptor marker CRX, compared to levels in the control group. These cells also expressed lower levels of the axon markers TUBB3 and NEFL than cells in the control group. In DEX-treatment group cells, dendrite, retinal progenitor, and photoreceptor markers (MAP2, CHX10, and CRX) were upregulated compared to levels in the control group. (b) However, 4 weeks after immunosuppressant treatment, both RAP- and DEX-treated cells showed higher expression levels of only PAX6 and CRX as compared to those in the control group. (c) Representative histogram of flow cytometry results. Each wave represented one group; the proportion of each group was calculated by the Blk group. There were 79.87 ± 5.92% of the RGCs in the control group, 60.97 ± 7.36% in the RAP group, and 63.6 ± 10.35% in the DEX group. CTRL: control group; RAP: rapamycin-treatment group; DEX: dexamethasone-treatment group; iPSCs: negative control group in RT-PCR; Blk: blank control group in flow cytometry.

Article Snippet: Antibodies against the following markers were used as primary antibodies for immunofluorescence microscopy at the dilutions indicated in parentheses: Ki67 (1 : 100, A11390, ABclonal, China), CHX10 (1 : 200, AB9016, Millipore, Germany), Brn3 (1 : 200, SC-6026X, Santa Cruz, USA), Islet1 (1 : 20, BM44446, Boster, China), HuD (1 : 100, SC-48421, Santa Cruz), RBPMS (1 : 100, ab152101, Abcam), β III-tubulin (1 : 200, ab7751, Abcam), neurofilament light polypeptide (NEFL; 1 : 100, A0257, ABclonal), microtubule-associated protein 2 (MAP2, 1 : 100, BM1243, Boster), vimentin (1 : 50, BM4029, Boster), glial fibrillary acidic protein (GFAP; 1 : 500, 3670S, Cell Signaling Technology, USA), CD68 (1 : 200, ab201340, Abcam), Iba1 (1 : 200, ab5076, Abcam), and SC121 (1 : 500, Y40410, Takara Bio).

Techniques: Marker, Expressing, Control, Flow Cytometry, Negative Control, Reverse Transcription Polymerase Chain Reaction

Journal: Molecular Genetics and Metabolism Reports

Article Title: Generation of GLA-knockout human embryonic stem cell lines to model peripheral neuropathy in Fabry disease

doi: 10.1016/j.ymgmr.2022.100914

Figure Lengend Snippet: Antibodies used for immunostaining.

Article Snippet: Islet1/ISL1 , Islet1/ISL1 , rabbit IgG , 1:500 , Sino Biological , Wayne, PA.

Techniques: Immunostaining

Journal: Molecular Genetics and Metabolism Reports

Article Title: Generation of GLA-knockout human embryonic stem cell lines to model peripheral neuropathy in Fabry disease

doi: 10.1016/j.ymgmr.2022.100914

Figure Lengend Snippet: Immunostaining of neuronal cells derived from AGA-deficient WA14 clones for markers of sensory neurons. WA14 clones were differentiated as described in Methods. At Day 8, they were transferred to Matrigel-coated 2-well glass chamber slides and the differentiation protocol was continued to Day 12, after which the cells were refed with N2 growth medium. At Day 14, the cultures were fixed and stained as described in Methods. (A) Cells were double stained for peripherin (green) and beta-III-tubulin (TUBB3, red). Areas of overlap appear as yellow. (B) Cells were double stained for BRN3A (green) and beta-III-tubulin (TUJ1, red). (C) Cells were double stained for Islet1 (green) and beta-III-tubulin (TUJ1, red). All slides were imaged in a Keyence 9000 microscope using a 20× objective. Scale bars = 100 μm.

Article Snippet: Islet1/ISL1 , Islet1/ISL1 , rabbit IgG , 1:500 , Sino Biological , Wayne, PA.

Techniques: Immunostaining, Derivative Assay, Clone Assay, Staining, Microscopy