Journal: ACS nano

Article Title: Echinacoside-Zinc Nanomaterial Inhibits Skin Glycation by Suppressing the Transcriptional Activation of the Receptor for Advanced Glycation End-Products.

doi: 10.1021/acsnano.3c04726

Figure Lengend Snippet: Figure 2. PPZn exerted anti-antiglycan in glycated model mouse skin. (A−C) Representative images of immunohistochemical (IHC) staining (A−a), HE staining (B−a), and Masson staining (C−a) of mouse skin tissue after the indicated treatment. The statistical results of the IHC staining index (A−b), epidermal thickness (B−b), and collagen density (C−b) are shown on the right. Scale bar, 50 μm. (D) qRT- PCR detection of RAGE mRNA levels in skin tissues of mice after the indicated treatment. (E) Western blot representative images and quantitative analysis of RAGE, COL1A2, MMP1, AGEs, and β-actin protein levels in skin tissues of mice after the indicated treatment. (F) Representative images of TUNEL staining of mouse skin tissue after the indicated treatment. Scale bar, 50 μm. (G−I) Relative Hyp content (G), SOD activity (H), and MDA concentration (I) in skin tissues of mice after the indicated treatment. All values are presented as the mean ± SD; P-values determined by two-sided Student’s t test. *P < 0.05, **P < 0.01, compared with the model.

Article Snippet: After being blocked, samples were then incubated with primary antibodies against MDM2 (Proteintech, 1:200), RAGE (Santa, 1:50), STAT2 (Zenbio, 1:100), MMP1 (Affinity, 1:100), COL1A2 (Proteintech, 1:100), and AGEs (Abcam, 1:100) at 4 °C overnight.

Techniques: Immunohistochemical staining, Immunohistochemistry, Staining, Quantitative RT-PCR, Western Blot, TUNEL Assay, Activity Assay, Concentration Assay

Journal: ACS nano

Article Title: Echinacoside-Zinc Nanomaterial Inhibits Skin Glycation by Suppressing the Transcriptional Activation of the Receptor for Advanced Glycation End-Products.

doi: 10.1021/acsnano.3c04726

Figure Lengend Snippet: Figure 3. PPZn exerted antiglycation effects in HaCaT cells. (A) Western blot representative images and quantitative analysis of RAGE, COL1A2, MMP1, AGEs, and β-actin protein levels in HaCaT cells after the indicated treatment. (B) Cell cycle was determined by flow cytometry in HaCaT cells after the indicated treatment. (C) Representative images of TUNEL staining of HaCaT cells after the indicated treatment. Scale bar, 50 μm. (D, E) Relative MDA concentration (D) and SOD activity (E) in HaCaT cells after the indicated treatment. All values are presented as the mean ± SD; P-values determined by two-sided Student’s t test. *P < 0.05, **P < 0.01, compared with the model.

Article Snippet: After being blocked, samples were then incubated with primary antibodies against MDM2 (Proteintech, 1:200), RAGE (Santa, 1:50), STAT2 (Zenbio, 1:100), MMP1 (Affinity, 1:100), COL1A2 (Proteintech, 1:100), and AGEs (Abcam, 1:100) at 4 °C overnight.

Techniques: Western Blot, Flow Cytometry, TUNEL Assay, Staining, Concentration Assay, Activity Assay

Journal: Immunity, Inflammation and Disease

Article Title: Electroacupuncture of Guanyuan (CV4) Acupoint Improved Pelvic Inflammatory Disease Pain by Inhibiting Neuroinflammation and Sympathetic Activity

doi: 10.1002/iid3.70299

Figure Lengend Snippet: EA stimulation of Guanyuan acupoint reduces the expression of pain‐causing substances and inflammatory mediators in rats with PID. 5‐HT3R, CGRP, HA, KNG1, PGI2, and TFF2 expressions in EA stimulation of Guanyuan acupoint skin (A) and plantar skin (B) were detected using western blot analysis. Values are expressed as mean ± SD ( n = 6), ** p < 0.01, versus control group; # p < 0.05, ## p < 0.01, ns p > 0.05, versus model group. 5‐HT3R, 5‐hydroxytryptamine receptor 3; CGRP, calcitonin‐gene‐related peptide; HA, hyaluronic acid; KNG1, Kininogen 1; PGI2, prostaglandin I2; TFF2, trefoil factor 2.

Article Snippet: The antibodies used were as follows: β‐actin (AC026, Abclonal, 1:50,000); 5‐HT3R (BS‐34076R, Bioss, 1:2,000); CGRP (A5542, Abclonal, 1:1,000); HA Tag (AF0039, Beyotime, 1:1,000); KNG1 (DF6544, Affinity, 1:2,000); PGI2 (ZY64571R, Shzeye, 1:2,000); TFF2 (66471‐1‐Ig, Proteintech, 1:1,000); HRP Goat Anti‐Mouse IgG (H + L) (AS003, Abclonal, 1:5,000); and Goat Anti‐Rabbit IgG (H + L) HRP (S0001, Affbiotech, 1:5,000).

Techniques: Expressing, Western Blot, Control