Journal: International Journal of Molecular Sciences

Article Title: Quality Analysis of Minerals Formed by Jaw Periosteal Cells under Different Culture Conditions

doi: 10.3390/ijms20174193

Figure Lengend Snippet: Detection of JPCs mineralization (Alizarin Red staining) in AFM and Raman petri dishes under FCS and hPL supplementation. Macroscopic images: JPC mineralization from three donors (#1, #2, #3) cultured in AFM (plastic bottom) and Raman (glass bottom) petri dishes was observed after osteogenic induction for 25 days under both medium supplementations ( A ). Quantification of JPC mineralization degrees in cell monolayers from the three donors ( B ). Calcium levels detected in cell monolayers cultured in AFM dishes are represented by red columns and those in Raman petri dishes by blue columns. Abbreviations: AFM—atomic force microscopy, FCS—fetal calf serum, hPL—human platelet lysate, * p < 0.05.

Article Snippet: Elastic moduli of JPCs were assessed ( n = 4 donors) using an AFM system (CellHesion 200, JPK Instruments, Berlin, Germany) mounted on to an inverted light microscope (AxioObserver D1, Carl Zeiss Microscopy, Jena, Germany), which allowed simultaneous visualization of the cells.

Techniques: Staining, Cell Culture, Microscopy

Journal: International Journal of Molecular Sciences

Article Title: Quality Analysis of Minerals Formed by Jaw Periosteal Cells under Different Culture Conditions

doi: 10.3390/ijms20174193

Figure Lengend Snippet: Representative microscopic images indicating the regions of interest subjected to elasticity measurements. Microscopic pictures of AFM measured JPCs—control monolayers (left pictures), osteogenic induced JPCs (middle) and calcium phosphate precipitates (right pictures) formed under hPL and FCS culturing conditions, respectively. Red arrows depict the position of the cantilever for elasticity measurements of non-mineralized regions in untreated (left) and osteogenic monolayers (middle, without precipitates) and of calcium phosphate precipitates. Images were acquired with the inverted AxioObserver D1 light microscope attached to the AFM system at a 10× magnification. Scale bar (black) represents 30 µm. Abbreviations: AFM—atomic force microscopy, JPCs—jaw periosteum derived progenitor cells, FCS—fetal calf serum, hPL—human platelet lysate.

Article Snippet: Elastic moduli of JPCs were assessed ( n = 4 donors) using an AFM system (CellHesion 200, JPK Instruments, Berlin, Germany) mounted on to an inverted light microscope (AxioObserver D1, Carl Zeiss Microscopy, Jena, Germany), which allowed simultaneous visualization of the cells.

Techniques: Control, Light Microscopy, Microscopy, Derivative Assay

Journal: bioRxiv

Article Title: Biomechanical and biochemical assessment of YB-1 expression in melanoma cells

doi: 10.1101/2021.12.29.474412

Figure Lengend Snippet: ( A ) Cell lines A375 parental and A375 YB-1 knock-out as well as MelJuso parental were subjected to AFM measurements. White arrows indicate the cantilever employed for indentations. Blue arrows show the region of interest in an exemplary cell. ( B ) Schematic drawing of a single cell indentation by a cantilever. Images were acquired with the inverted AxioObserver D1 light microscope attached to the AFM system at a 10× magnification. Scale bar (black) represents 30 µm. Abbreviations: AFM -atomic force microscopy, PA -parental, KO - knock-out.

Article Snippet: To measure the stiffness of cells, we used an atomic force microscope (AFM) system (CellHesion 200, JPK Instruments, Berlin, Germany) mounted onto an inverted light microscope (AxioObserver D1, Carl Zeiss Microscopy, Jena, Germany), which allowed us to optically align the cantilever to the region of interest and to simultaneously measure specific regions of interest on individual cells ( ).

Techniques: Knock-Out, Light Microscopy, Microscopy

Journal: Frontiers in Molecular Medicine

Article Title: Biomechanical and biochemical assessment of YB-1 expression in A375 melanoma cell line: Exploratory study

doi: 10.3389/fmmed.2023.1050487

Figure Lengend Snippet: Microscopic images representing the regions of interest subjected to AFM measurements. (A) Cell lines A375 parental and A375 YB-1 knock-out, as well as MelJuso parental , were subjected to AFM measurements. White arrows indicate the cantilever employed for indentation. Black arrows show the region of interest to be measured - an exemplary cell. (B) Schematic drawing of a single cell indented by a spherical AFM cantilever (5 µm). Images were acquired with the inverted AxioObserver D1 light microscope attached to the AFM system at × 10 magnification. The scale bar (black) represents 30 µm. Abbreviations: AFM - atomic force microscopy, PA - parental, KO - knock-out.

Article Snippet: To measure the stiffness of cells, we used an atomic force microscope (AFM) system (CellHesion 200, JPK Instruments, Berlin, Germany) mounted onto an inverted light microscope (AxioObserver D1, Carl Zeiss Microscopy, Jena, Germany), which allowed us to optically align the cantilever to the region of interest and to simultaneously measure specific regions of interest on individual cells ( ).

Techniques: Knock-Out, Light Microscopy, Microscopy

Journal: International Journal of Molecular Sciences

Article Title: Injection of Porcine Adipose Tissue-Derived Stromal Cells by a Novel Waterjet Technology

doi: 10.3390/ijms22083958

Figure Lengend Snippet: Representative images indicating the regions of interest subjected to elasticity measurements via AFM. Microscopic pictures of AFM-measured porcine adipose tissue-derived stromal cells (pADSCs) control monolayers (left pictures) and pADSCs injected by WN (middle pictures) or WJ (right pictures). Following WN and WJ injection, cells were collected, washed by PBS, and allowed to attach for 3 h in expansion medium before AFM measurements. As controls, cells that were not subjected to an injection procedure were used. The cantilever employed for measurements is also displayed (white star). Representative regions containing cells subjected to AFM measurements are also displayed (white squares). Images were acquired with the inverted AxioObserver D1 light microscope attached to the AFM system at a 10× magnification. Scale bar (black) represents 100 µm. Abbreviations: WJ—waterjet, WN—Williams needle.

Article Snippet: Briefly, an AFM system (CellHesion 200, Bruker, Billerica, MA, USA) equipped with an inverted microscope (AxioObserver D1, Carl Zeiss AG, Jena, Germany) was employed.

Techniques: Derivative Assay, Control, Injection, Light Microscopy