invected Search Results


anti-engrailed/invected gene products  (Developmental Studies Hybridoma Bank)
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monoclonal anti engrailed 4d9  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology monoclonal anti engrailed 4d9
Fig. 1. Pioneer and MFF precursors develop in close proximity to Laminin-rich extracellular matrices and Engrailed expression is absent from pioneers and MFFs in sly. (A,B)Transverse sections of 20 s stage wild-type (A) and sly embryos (B) at the level of early adaxial cell stacking. Dorsal is upwards. F59 (in green) labels adaxial cells and Laminin is in red; nuclei are counterstained with DAPI (in blue). (C-F)Transverse sections of 26 hpf wild-type (C,E) and sly (D,F) embryos at the level of the anterior tail (C,D) and the anterior trunk (E,F). (C,D)Laminin is in red. Owing to the somite chevron shape, vertical myosepta of the neighbour somite (blue arrows) are visible on these transverse sections. <t>(C-F)4D9</t> (in green) labels pioneers (arrowheads, C,E) and MFFs (arrows, E) in wild-type embryos and is absent in sly embryos (D,F). n, notochord; nt, neural tube. Scale bar: 50mm.
Monoclonal Anti Engrailed 4d9, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/invected/engrailed%2Finvected+Antibody/pm21115608-64-38-48
Average 90 stars, based on 1 article reviews
monoclonal anti engrailed 4d9 - by Bioz Stars, 2026-09
90/100 stars
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invect transfection reagent  (EMP Biotech)
90
EMP Biotech invect transfection reagent
Overview of performed optimizations of TGE parameters . (a) Yield of plasmid preparation for transient <t>transfection.</t> (b) Antibody quantities (7 day post transfection) expressed by HEK-293 using indicated media. (c) Antibody quantities (7 day post transfection) expressed by HEK-293 and proprietary HEK-INV. (d) Antibody quantities (7 day post transfection) expressed by HEK-293 using indicated transfection reagent.
Invect Transfection Reagent, supplied by EMP Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/invected/invect+transfection+reagent/pmc04685322-15-12-18
Average 90 stars, based on 1 article reviews
invect transfection reagent - by Bioz Stars, 2026-09
90/100 stars
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celsus s invectives  (Celsus Laboratories)
86
Celsus Laboratories celsus s invectives
Overview of performed optimizations of TGE parameters . (a) Yield of plasmid preparation for transient <t>transfection.</t> (b) Antibody quantities (7 day post transfection) expressed by HEK-293 using indicated media. (c) Antibody quantities (7 day post transfection) expressed by HEK-293 and proprietary HEK-INV. (d) Antibody quantities (7 day post transfection) expressed by HEK-293 using indicated transfection reagent.
Celsus S Invectives, supplied by Celsus Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/invected/celsus+invectives+s/10__47456_slash_pbnj0f48-18-11-11
Average 86 stars, based on 1 article reviews
celsus s invectives - by Bioz Stars, 2026-09
86/100 stars
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Image Search Results


Fig. 1. Pioneer and MFF precursors develop in close proximity to Laminin-rich extracellular matrices and Engrailed expression is absent from pioneers and MFFs in sly. (A,B)Transverse sections of 20 s stage wild-type (A) and sly embryos (B) at the level of early adaxial cell stacking. Dorsal is upwards. F59 (in green) labels adaxial cells and Laminin is in red; nuclei are counterstained with DAPI (in blue). (C-F)Transverse sections of 26 hpf wild-type (C,E) and sly (D,F) embryos at the level of the anterior tail (C,D) and the anterior trunk (E,F). (C,D)Laminin is in red. Owing to the somite chevron shape, vertical myosepta of the neighbour somite (blue arrows) are visible on these transverse sections. (C-F)4D9 (in green) labels pioneers (arrowheads, C,E) and MFFs (arrows, E) in wild-type embryos and is absent in sly embryos (D,F). n, notochord; nt, neural tube. Scale bar: 50mm.

Journal: Development (Cambridge, England)

Article Title: Laminins, via heparan sulfate proteoglycans, participate in zebrafish myotome morphogenesis by modulating the pattern of Bmp responsiveness.

doi: 10.1242/dev.053975

Figure Lengend Snippet: Fig. 1. Pioneer and MFF precursors develop in close proximity to Laminin-rich extracellular matrices and Engrailed expression is absent from pioneers and MFFs in sly. (A,B)Transverse sections of 20 s stage wild-type (A) and sly embryos (B) at the level of early adaxial cell stacking. Dorsal is upwards. F59 (in green) labels adaxial cells and Laminin is in red; nuclei are counterstained with DAPI (in blue). (C-F)Transverse sections of 26 hpf wild-type (C,E) and sly (D,F) embryos at the level of the anterior tail (C,D) and the anterior trunk (E,F). (C,D)Laminin is in red. Owing to the somite chevron shape, vertical myosepta of the neighbour somite (blue arrows) are visible on these transverse sections. (C-F)4D9 (in green) labels pioneers (arrowheads, C,E) and MFFs (arrows, E) in wild-type embryos and is absent in sly embryos (D,F). n, notochord; nt, neural tube. Scale bar: 50mm.

Article Snippet: Antibodies used were: monoclonal anti-Myosin F59 (1:5 on sections; DSHB), polyclonal anti-Laminin (1:400 on sections and 1:200 in toto; Sigma, catalog number L-9393), polyclonal anti-phospho-Smad1/5/8 (1:100 on sections and 1:50 in toto; Cell Signaling Technology, catalog number 9511), monoclonal anti-Engrailed 4D9 (1:50 on sections and 1:25 in toto; Santa Cruz Biotechnology, clone sc-53019), polyclonal anti-Prox1 (1:100 in toto; RELIATech GmbH, catalog number 102-PA32AG), monoclonal anti-heparan sulfate (1:200 on sections; US Biological, 10E4 epitope, catalog number H1890), monoclonal anti-chondroitin sulfate (1:200 on sections; Sigma, clone CS-56, catalog number C8035).

Techniques: Expressing

Fig. 3. pSmad expression is precisely regulated in pioneers and their precursors in wild-type, sly and cyclopamine-treated embryos. (A-E)Transverse sections of 18-20 s stage wild-type (A,B), sly (C,D) and cyclopamine- treated embryos (E) at the level of adaxial cell pre-stacking (A,C,E) and stacking (B,D). Dorsal is upwards. F59 (in red) labels adaxial cells and nuclear pSmad labelling is in green; nuclei are counterstained with DAPI (in blue). (F)Lateral view (projection of 11 confocal sections, 0,6mm z-step) of a 18- 20 s stage wild-type embryo, at the trunk level. Anterior is on the left, dorsal is upwards. 4D9 (in red) labels pioneers and nuclear pSmad labelling is in green. n, notochord; nt, neural tube. Scale bar: 50mm.

Journal: Development (Cambridge, England)

Article Title: Laminins, via heparan sulfate proteoglycans, participate in zebrafish myotome morphogenesis by modulating the pattern of Bmp responsiveness.

doi: 10.1242/dev.053975

Figure Lengend Snippet: Fig. 3. pSmad expression is precisely regulated in pioneers and their precursors in wild-type, sly and cyclopamine-treated embryos. (A-E)Transverse sections of 18-20 s stage wild-type (A,B), sly (C,D) and cyclopamine- treated embryos (E) at the level of adaxial cell pre-stacking (A,C,E) and stacking (B,D). Dorsal is upwards. F59 (in red) labels adaxial cells and nuclear pSmad labelling is in green; nuclei are counterstained with DAPI (in blue). (F)Lateral view (projection of 11 confocal sections, 0,6mm z-step) of a 18- 20 s stage wild-type embryo, at the trunk level. Anterior is on the left, dorsal is upwards. 4D9 (in red) labels pioneers and nuclear pSmad labelling is in green. n, notochord; nt, neural tube. Scale bar: 50mm.

Article Snippet: Antibodies used were: monoclonal anti-Myosin F59 (1:5 on sections; DSHB), polyclonal anti-Laminin (1:400 on sections and 1:200 in toto; Sigma, catalog number L-9393), polyclonal anti-phospho-Smad1/5/8 (1:100 on sections and 1:50 in toto; Cell Signaling Technology, catalog number 9511), monoclonal anti-Engrailed 4D9 (1:50 on sections and 1:25 in toto; Santa Cruz Biotechnology, clone sc-53019), polyclonal anti-Prox1 (1:100 in toto; RELIATech GmbH, catalog number 102-PA32AG), monoclonal anti-heparan sulfate (1:200 on sections; US Biological, 10E4 epitope, catalog number H1890), monoclonal anti-chondroitin sulfate (1:200 on sections; Sigma, clone CS-56, catalog number C8035).

Techniques: Expressing

Fig. 4. pSmad expression in MFFs and their precursors is regulated in a similar way to that in pioneers. (A-F)Transverse sections of 26 hpf wild-type (A-C) and sly (D-F) embryos at the level of the posterior tail (A,D), the anterior tail (B,E) and the anterior trunk (C,F). Dorsal is upwards. Nuclear pSmad labelling is in green. Anti- pSmad antibody decorates myofibrils in differentiating slow and fast muscle fibres (C,F). (B,E)F59 (in red) labels migrating slow muscle fibres. (C,F)4D9 (in red) labels pioneers (arrowhead) and MFFs (arrow). The horizontal white lines delimitate the pSmad-negative central domain. n, notochord; nt, neural tube. Scale bar: 50mm.

Journal: Development (Cambridge, England)

Article Title: Laminins, via heparan sulfate proteoglycans, participate in zebrafish myotome morphogenesis by modulating the pattern of Bmp responsiveness.

doi: 10.1242/dev.053975

Figure Lengend Snippet: Fig. 4. pSmad expression in MFFs and their precursors is regulated in a similar way to that in pioneers. (A-F)Transverse sections of 26 hpf wild-type (A-C) and sly (D-F) embryos at the level of the posterior tail (A,D), the anterior tail (B,E) and the anterior trunk (C,F). Dorsal is upwards. Nuclear pSmad labelling is in green. Anti- pSmad antibody decorates myofibrils in differentiating slow and fast muscle fibres (C,F). (B,E)F59 (in red) labels migrating slow muscle fibres. (C,F)4D9 (in red) labels pioneers (arrowhead) and MFFs (arrow). The horizontal white lines delimitate the pSmad-negative central domain. n, notochord; nt, neural tube. Scale bar: 50mm.

Article Snippet: Antibodies used were: monoclonal anti-Myosin F59 (1:5 on sections; DSHB), polyclonal anti-Laminin (1:400 on sections and 1:200 in toto; Sigma, catalog number L-9393), polyclonal anti-phospho-Smad1/5/8 (1:100 on sections and 1:50 in toto; Cell Signaling Technology, catalog number 9511), monoclonal anti-Engrailed 4D9 (1:50 on sections and 1:25 in toto; Santa Cruz Biotechnology, clone sc-53019), polyclonal anti-Prox1 (1:100 in toto; RELIATech GmbH, catalog number 102-PA32AG), monoclonal anti-heparan sulfate (1:200 on sections; US Biological, 10E4 epitope, catalog number H1890), monoclonal anti-chondroitin sulfate (1:200 on sections; Sigma, clone CS-56, catalog number C8035).

Techniques: Expressing

Fig. 5. Knockdown of Bmp signal rescues Engrailed expression in sly pioneers and MFFs and ectopically activates Engrailed expression in wild-type slow and fast lineages. (A,E,I,M) Lateral views of wild-type (A), sly (E), dorsomorphin-treated sly (I) and dorsomorphin-treated wild- type embryos (M) at 26 hpf. Anterior is leftwards, dorsal is upwards. (B-D,F-H,J-L,N-P) Magnification views (projection of confocal sections) of the regions around somite 10 (B-C,F-G,J-K,N-O) and somite 18 (D,H,L,P) for each type of embryo. (A-P)4D9 (in green) labels pioneer (arrowheads) and MFF nuclei; Prox1 (in red) labels slow muscle fibre nuclei, including pioneers. Because of the projection, some nuclei artificially overlap. All pioneer nuclei are therefore identified by arrowheads. (A,C,G,I,K,O) 4D9 labelling only. The asterisk locates ectopic tails in dorsomorphin-treated embryos. Scale bar: 50mm.

Journal: Development (Cambridge, England)

Article Title: Laminins, via heparan sulfate proteoglycans, participate in zebrafish myotome morphogenesis by modulating the pattern of Bmp responsiveness.

doi: 10.1242/dev.053975

Figure Lengend Snippet: Fig. 5. Knockdown of Bmp signal rescues Engrailed expression in sly pioneers and MFFs and ectopically activates Engrailed expression in wild-type slow and fast lineages. (A,E,I,M) Lateral views of wild-type (A), sly (E), dorsomorphin-treated sly (I) and dorsomorphin-treated wild- type embryos (M) at 26 hpf. Anterior is leftwards, dorsal is upwards. (B-D,F-H,J-L,N-P) Magnification views (projection of confocal sections) of the regions around somite 10 (B-C,F-G,J-K,N-O) and somite 18 (D,H,L,P) for each type of embryo. (A-P)4D9 (in green) labels pioneer (arrowheads) and MFF nuclei; Prox1 (in red) labels slow muscle fibre nuclei, including pioneers. Because of the projection, some nuclei artificially overlap. All pioneer nuclei are therefore identified by arrowheads. (A,C,G,I,K,O) 4D9 labelling only. The asterisk locates ectopic tails in dorsomorphin-treated embryos. Scale bar: 50mm.

Article Snippet: Antibodies used were: monoclonal anti-Myosin F59 (1:5 on sections; DSHB), polyclonal anti-Laminin (1:400 on sections and 1:200 in toto; Sigma, catalog number L-9393), polyclonal anti-phospho-Smad1/5/8 (1:100 on sections and 1:50 in toto; Cell Signaling Technology, catalog number 9511), monoclonal anti-Engrailed 4D9 (1:50 on sections and 1:25 in toto; Santa Cruz Biotechnology, clone sc-53019), polyclonal anti-Prox1 (1:100 in toto; RELIATech GmbH, catalog number 102-PA32AG), monoclonal anti-heparan sulfate (1:200 on sections; US Biological, 10E4 epitope, catalog number H1890), monoclonal anti-chondroitin sulfate (1:200 on sections; Sigma, clone CS-56, catalog number C8035).

Techniques: Knockdown, Expressing

Fig. 6. Heparan sulfate proteoglycans are absent in sly embryos and Heparinase III treatment affects Engrailed and pSmad expression in a manner similar to the sly mutant. (A-D)Transverse sections of wild-type (A,C) and sly (B,D) embryos at the 20 s stage (A,B, adaxial cell stacking level) and 26 hpf (C,D, anterior tail level). Dorsal is upwards. In addition to its extracellular signal, anti-HS antibody (in green) labels all nuclei. (E,I)Lateral views of vehicle solution-injected wild-type (E) and Heparinase III-injected wild-type (I) embryos at 26 hpf. Anterior is leftwards, dorsal is upwards. (F-H,J-L) Magnification views (projection of confocal sections) of the regions around somite 10 (F-G,J-K) and somite 18 (H,L) for each type of embryo. (E-L)4D9 (in green) labels pioneer (arrowheads) and MFF nuclei; Prox1 (in red) labels slow muscle fibre nuclei, including pioneers. Because of the projection, some nuclei artificially overlap. All pioneer nuclei are therefore identified by arrowheads. (E,G,I,K) 4D9 labelling only. (M-P)Transverse sections of vehicle solution-injected wild-type (M,O) and Heparinase III-injected wild-type (N,P) embryos at the 20 s stage (M,N, adaxial cell level) and 26 hpf (O,P, anterior tail level). Dorsal is upwards. (M-P)Nuclear pSmad labelling is in green. (M,N)Nuclei are counterstained with DAPI (in blue). (O,P)4D9 (in red) labels pioneers. The horizontal white lines delimitate the pSmad-negative central domain. n, notochord; nt, neural tube. Scale bar: 50mm.

Journal: Development (Cambridge, England)

Article Title: Laminins, via heparan sulfate proteoglycans, participate in zebrafish myotome morphogenesis by modulating the pattern of Bmp responsiveness.

doi: 10.1242/dev.053975

Figure Lengend Snippet: Fig. 6. Heparan sulfate proteoglycans are absent in sly embryos and Heparinase III treatment affects Engrailed and pSmad expression in a manner similar to the sly mutant. (A-D)Transverse sections of wild-type (A,C) and sly (B,D) embryos at the 20 s stage (A,B, adaxial cell stacking level) and 26 hpf (C,D, anterior tail level). Dorsal is upwards. In addition to its extracellular signal, anti-HS antibody (in green) labels all nuclei. (E,I)Lateral views of vehicle solution-injected wild-type (E) and Heparinase III-injected wild-type (I) embryos at 26 hpf. Anterior is leftwards, dorsal is upwards. (F-H,J-L) Magnification views (projection of confocal sections) of the regions around somite 10 (F-G,J-K) and somite 18 (H,L) for each type of embryo. (E-L)4D9 (in green) labels pioneer (arrowheads) and MFF nuclei; Prox1 (in red) labels slow muscle fibre nuclei, including pioneers. Because of the projection, some nuclei artificially overlap. All pioneer nuclei are therefore identified by arrowheads. (E,G,I,K) 4D9 labelling only. (M-P)Transverse sections of vehicle solution-injected wild-type (M,O) and Heparinase III-injected wild-type (N,P) embryos at the 20 s stage (M,N, adaxial cell level) and 26 hpf (O,P, anterior tail level). Dorsal is upwards. (M-P)Nuclear pSmad labelling is in green. (M,N)Nuclei are counterstained with DAPI (in blue). (O,P)4D9 (in red) labels pioneers. The horizontal white lines delimitate the pSmad-negative central domain. n, notochord; nt, neural tube. Scale bar: 50mm.

Article Snippet: Antibodies used were: monoclonal anti-Myosin F59 (1:5 on sections; DSHB), polyclonal anti-Laminin (1:400 on sections and 1:200 in toto; Sigma, catalog number L-9393), polyclonal anti-phospho-Smad1/5/8 (1:100 on sections and 1:50 in toto; Cell Signaling Technology, catalog number 9511), monoclonal anti-Engrailed 4D9 (1:50 on sections and 1:25 in toto; Santa Cruz Biotechnology, clone sc-53019), polyclonal anti-Prox1 (1:100 in toto; RELIATech GmbH, catalog number 102-PA32AG), monoclonal anti-heparan sulfate (1:200 on sections; US Biological, 10E4 epitope, catalog number H1890), monoclonal anti-chondroitin sulfate (1:200 on sections; Sigma, clone CS-56, catalog number C8035).

Techniques: Expressing, Mutagenesis, Injection

Overview of performed optimizations of TGE parameters . (a) Yield of plasmid preparation for transient transfection. (b) Antibody quantities (7 day post transfection) expressed by HEK-293 using indicated media. (c) Antibody quantities (7 day post transfection) expressed by HEK-293 and proprietary HEK-INV. (d) Antibody quantities (7 day post transfection) expressed by HEK-293 using indicated transfection reagent.

Journal: BMC Proceedings

Article Title: Reconciling pillars of transient gene expression: From DNA prep via media, reagent and cell line development to holistic process optimization

doi: 10.1186/1753-6561-9-S9-P18

Figure Lengend Snippet: Overview of performed optimizations of TGE parameters . (a) Yield of plasmid preparation for transient transfection. (b) Antibody quantities (7 day post transfection) expressed by HEK-293 using indicated media. (c) Antibody quantities (7 day post transfection) expressed by HEK-293 and proprietary HEK-INV. (d) Antibody quantities (7 day post transfection) expressed by HEK-293 using indicated transfection reagent.

Article Snippet: For screening approaches 5x10E6 cells/mL were transfected with 2 pg DNA/cell and INVect transfection reagent (Cat. No. FK-0101-M001.0-001, emp Biotech GmbH) with INVect to DNA ratio of 6:1 (w/w) or 25 kDa L-PEI with PEI to DNA ration of 2:1 (w/w) in 8 mL culture volume in 50 mL bioreactor tubes.

Techniques: Plasmid Preparation, Transfection