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Image Search Results
Journal: bioRxiv
Article Title: Development and Characterization of Recombinant Vesicular Stomatitis Virus (rVSV)-based Bivalent Vaccine Against COVID-19 Delta Variant and Influenza Virus
doi: 10.1101/2021.12.14.472657
Figure Lengend Snippet: The neutralization titers (50% inhibition) in immunized mice against SPΔC wt -Luc-PVs (A), SPΔCdelta-Luc-PVs (B), SPΔC beta’ -Luc-PVs (D) and SPΔC b.1.617 -Luc-PVs (E) after 28 days of prime-boost vaccination with different bivalent vaccines. VSV-G-Luc-PVPs (C) was used as negative control. The mouse serum of each immunization group collected at day 28 were pooled together, 2× serially diluted and incubated with different Luc-PVs (∼10 4 RLU). Then, the mixtures were added in A549 ACE2 . cell cultures and the infection of PVs was determined by Luciferase assay at 48∼66 hrs post infection. The percentage of infection was calculated compared with no serum control and neutralizing titers were calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0, as described in Materials and Methods. The neutralizing titers of V-EM2/SPΔC1 immunized mouse sera against different SPΔC-Luc-PVs were listed (F). Data represent Mean ±SD and were obtained from over three independent experiments. Statistical significance was determined using ordinary one-way ANOVA test and Turkey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.
Article Snippet: The endpoint titer is designated as the reciprocal of the highest dilution of a serum that has an OD450 above the cutoff (10× negative control) and is calculated by using
Techniques: Neutralization, Inhibition, Vaccines, Negative Control, Incubation, Infection, Luciferase, Control