Journal: Journal of Lipid Research

Article Title: KLF7 induced ADRB3-dependent IL-6 production in brown adipocytes during stress

doi: 10.1016/j.jlr.2026.100981

Figure Lengend Snippet: Stress induces KLF7 and IL-6 in BAT by activating ADRB3. A: Immunoblotting for p-CREB, CREB, KLF7, and IL-6 protein levels in the BAT of mice 4 h after retro-orbital bleeding. Quantification of band intensity was performed by densitometric analysis, normalized to β-Tubulin. Band intensities for p-CREB were normalized to total CREB. B: mRNA levels of the Il-6 gene in the BAT of mice. C: Plasma IL-6 levels in mice after bleeding and injection of the ADRB3 antagonist SR59203A. D: Immunoblotting for p-PKA substrates in the BAT of mice. E: Klf7 mRNA expression in the BAT of mice after bleeding. F: Blood glucose levels of mice after bleeding. G: PTT was performed in mice 4 h after bleeding. AUC, area under the curve. H: Gluconeogenesis-associated gene expression in the liver of mice after bleeding. I: Protein levels of the p-PKA substrate in mice 2 h post injection of the ADRB3 agonist CL316,243 and the ADRB3 antagonist SR59203A. J: Immunoblotting for p-CREB, CREB, KLF7, and IL-6. K: Quantification of KLF7 and IL-6 protein expression levels by grayscale value analysis. L and M: mRNA levels of Klf7 and Il-6 in mice 2 h after administration of CL316,243 and SR59203A. N: Circulating IL-6 levels in mice postinjection. O: mRNA levels of gluconeogenesis-associated genes in the liver. n = 5 per group. Western blot quantification represents densitometric analysis from three independent biological samples. The data are presented as means ± SD. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. BAT, brown adipose tissue; IL-6, interleukin-6; KLF7, Krüppel-like factor 7; PKA, protein kinase A; CREB, cAMP response element-binding protein; PTT, pyruvate tolerance test.

Article Snippet: IL-6 levels in serum and cell culture medium were measured using a mouse IL-6 ELISA kit (Proteintech, KE10007, China) following the manufacturer's instructions.

Techniques: Western Blot, Clinical Proteomics, Injection, Expressing, Gene Expression, Binding Assay

Journal: Journal of Lipid Research

Article Title: KLF7 induced ADRB3-dependent IL-6 production in brown adipocytes during stress

doi: 10.1016/j.jlr.2026.100981

Figure Lengend Snippet: ADRB3 activation increases KLF7 and IL-6 expression in brown adipocytes. A: Phosphorylation of the PKA substrate in primary brown adipocytes treated with 5 μM CL316,243, normalized to β-Tubulin. B: CREB phosphorylation in brown adipocytes, normalized to total CREB. C: Immunoblotting and densitometric quantification of KLF7 and IL-6 protein levels in CL316,243-treated primary brown adipocytes. D: mRNA expression of Klf7 and Il-6 . E: IL-6 levels in the medium. F: Gluconeogenesis-associated gene expression in Hepa 1–6 cells cultured with conditioned medium from primary brown adipocytes treated with CL316243 . Data are shown as mean ± SD from three independent biological experiments, each performed using independently isolated and differentiated primary brown adipocytes. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. IL-6, interleukin-6; KLF7, Krüppel-like factor 7; PKA, protein kinase A; CREB, cAMP response element-binding protein.

Article Snippet: IL-6 levels in serum and cell culture medium were measured using a mouse IL-6 ELISA kit (Proteintech, KE10007, China) following the manufacturer's instructions.

Techniques: Activation Assay, Expressing, Phospho-proteomics, Western Blot, Gene Expression, Cell Culture, Isolation, Binding Assay

Journal: Journal of Lipid Research

Article Title: KLF7 induced ADRB3-dependent IL-6 production in brown adipocytes during stress

doi: 10.1016/j.jlr.2026.100981

Figure Lengend Snippet: ADRB3-induced IL-6 expression requires Klf7 in adipocytes during stress. A: Representative genotyping results for WT and Klf7 AKO mice. B: Representative western blots and densitometric quantification showing KLF7 levels in the BAT of WT and Klf7 AKO mice. C: Klf7 mRNA levels in the BAT of WT and Klf7 AKO mice. D: The levels of IL-6 in Klf7 AKO or WT mice after bleeding. E: mRNA expression of IL-6 in BAT. F: Circulating IL-6 levels. G: Gluconeogenic gene expression in the livers of WT and Klf7 AKO mice. H: PTT was performed in mice after bleeding. I: Blood glucose levels in mice after bleeding (n = 5 per group). J: Protein levels of IL-6 in mice 2 h after administration of CL316,243. K: mRNA expression in the BAT of mice after treated with 5 mg/kg CL316,243. L: Circulating IL-6 levels. M: Gluconeogenesis-associated gene expression in the livers of WT and Klf7 AKO mice. NT, no treated. n = 5 per group. Western blot quantification represents densitometric analysis from three independent biological samples. The data are presented as means ± SD. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. BAT, brown adipose tissue; IL-6, interleukin-6; KLF7, Krüppel-like factor 7; Klf7 AKO, Klf7 -adipocyte knockout; PTT, pyruvate tolerance test.

Article Snippet: IL-6 levels in serum and cell culture medium were measured using a mouse IL-6 ELISA kit (Proteintech, KE10007, China) following the manufacturer's instructions.

Techniques: Expressing, Western Blot, Gene Expression, Knock-Out

Journal: Journal of Lipid Research

Article Title: KLF7 induced ADRB3-dependent IL-6 production in brown adipocytes during stress

doi: 10.1016/j.jlr.2026.100981

Figure Lengend Snippet: Stress-induced upregulation of KLF7 and IL-6 in BAT is mediated by PKA activation. A: Representative western blots and densitometric quantification of PKA substrate phosphorylation after bleeding and administration of the PKA inhibitor H89. B: p-CREB, CREB, KLF7, and IL-6 protein levels in BAT. C: Klf7 and Il-6 mRNA levels in the BAT of mice 4 h after retro-orbital bleeding and the injection of the PKA inhibitor H89. D: Plasma IL-6 levels. E: Gluconeogenesis-associated gene expression in the liver of mice after bleeding. F: PTT was performed in mice 4 h after bleeding. G: Blood glucose levels in mice after bleeding. n = 5 per group. H and I: PKA substrate and CREB phosphorylation in the BAT of mice after the injection of CL316,243 and H89. J and K: mRNA and protein levels of Klf7 and Il-6 . L: Plasma levels of IL-6. M: mRNA levels of gluconeogenic genes in the liver. n = 5 per group. Western blot quantification represents densitometric analysis from three independent biological samples. The data are presented as means ± SD. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. BAT, brown adipose tissue; IL-6, interleukin-6; KLF7, Krüppel-like factor 7; PKA, protein kinase A; CREB, cAMP response element-binding protein; PTT, pyruvate tolerance test.

Article Snippet: IL-6 levels in serum and cell culture medium were measured using a mouse IL-6 ELISA kit (Proteintech, KE10007, China) following the manufacturer's instructions.

Techniques: Activation Assay, Western Blot, Phospho-proteomics, Injection, Clinical Proteomics, Gene Expression, Binding Assay

Journal: Journal of Lipid Research

Article Title: KLF7 induced ADRB3-dependent IL-6 production in brown adipocytes during stress

doi: 10.1016/j.jlr.2026.100981

Figure Lengend Snippet: PKA mediates the upregulation of KLF7 and IL-6 expression induced by ADRB3 activation and cAMP elevation in brown adipocytes. A: p-PKA substrate levels in primary brown adipocytes treated with CL316,243 and H89, normalized to β-tubulin. B: p-CREB, CREB, KLF7, and IL-6 protein levels in H89-pretreated primary brown adipocytes, normalized to β-tubulin. Band intensities for p-CREB were normalized to total CREB. C and D: K lf 7 and I l -6 mRNA expression. E: IL-6 levels in the medium of primary brown adipocytes treated with the indicated compounds (5 μM CL316243 or 10 μM H89). F: mRNA levels of gluconeogenesis-associated genes in Hepa 1–6 cells cultured with conditioned medium from primary brown adipocytes treated with the indicated compounds (5 μM CL316243 , 10 μM H89, or 2 μg/ml anti-IL-6 mAb). G: p-PKA substrate levels in primary brown adipocytes treated with 10 μM forskolin and 10 μM H89. H: p-CREB, CREB, KLF7, and IL-6 protein expression in primary brown adipocytes. I: mRNA expression of Klf7 in forskolin- and H89-treated primary brown adipocytes. J: Il-6 mRNA levels in primary brown adipocytes. K: IL-6 levels in the medium of brown adipocytes. L: Gluconeogenesis-associated gene expression levels in Hepa 1–6 cells cultured with conditioned medium from primary brown adipocytes treated with the indicated compounds (10 μM forskolin, 10 μM H89, or 2 μg/ml anti-IL-6 mAb). Data are shown as mean ± SD from three independent biological experiments, each performed using independently isolated and differentiated primary brown adipocytes. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. IL-6, interleukin-6; KLF7, Krüppel-like factor 7; PKA, protein kinase A; CREB, cAMP response element-binding protein.

Article Snippet: IL-6 levels in serum and cell culture medium were measured using a mouse IL-6 ELISA kit (Proteintech, KE10007, China) following the manufacturer's instructions.

Techniques: Expressing, Activation Assay, Cell Culture, Gene Expression, Isolation, Binding Assay

Journal: Journal of Lipid Research

Article Title: KLF7 induced ADRB3-dependent IL-6 production in brown adipocytes during stress

doi: 10.1016/j.jlr.2026.100981

Figure Lengend Snippet: CREB mediates ADRB3-induced KLF7 and IL-6 expression in brown adipocytes and transcriptionally activates KLF7. A: Immunoblotting for p-CREB, CREB, KLF7, and IL-6 in 666-15-pretreated primary brown adipocytes, normalized to β-tubulin. Band intensities for p-CREB were normalized to total CREB. B: Klf7 mRNA levels. C: Il-6 mRNA levels. D: IL-6 levels in the medium of primary brown adipocytes treated with the indicated compounds (5 μM CL316,243 and 10 μM 666-15). E: Binding site of CREB to KLF7 as predicted by the JASPAR database. F: Schematic of KLF7 fluorescent plasmids. G: KLF7 fluorescence activity values in CREB-overexpressing HEK-293T cells. H: ChIP assay performed in CREB-overexpressing HEK-293T cells. Data are shown as mean ± SD from three independent biological experiments performed using independently prepared primary brown adipocytes or HEK-293T cells. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. IL-6, interleukin-6; KLF7, Krüppel-like factor 7; CREB, cAMP response element-binding protein; ChIP, chromatin immunoprecipitation.

Article Snippet: IL-6 levels in serum and cell culture medium were measured using a mouse IL-6 ELISA kit (Proteintech, KE10007, China) following the manufacturer's instructions.

Techniques: Expressing, Western Blot, Binding Assay, Fluorescence, Activity Assay, Chromatin Immunoprecipitation

Journal: Advanced Science

Article Title: Neuroprotective Effects of Time‐Restricted Feeding Combined With Different Protein Sources in MPTP‐Induced Parkinson's Disease Mice Model and Its Modulatory Impact on Gut Microbiota Metabolism

doi: 10.1002/advs.202516502

Figure Lengend Snippet: MPTP‐induced PD‐like pathology depends on the source of dietary protein. (A) Representative IF image of TH (green) in the SNpc. Scale bar:100 µm (10×), 20 µm (40×). (B) Quantification of TH‐positive neuronal cells in the SNpc (cells/mm 2 ) (n = 5). (C) Representative western blotting of TH protein in the striatum. (D) Quantification of TH protein expression in the striatum (n = 6). (E–G) The concentration of striatal neurotransmitters and metabolites, including DA (E), HVA (F), and DOPAC (G) (n = 7). (H) Double IF staining for TH (dopaminergic neuron marker; green) and GFAP (astrocyte marker; red) in the SNpc. Scale bar:100 µm (10×), 20 µm (40×). (I) Quantitative analysis of the number of activated astrocytes in SNpc (cells mm −2 ) (n = 5). (J) Double IF staining for TH and Iba‐1 (microglial marker; red) in the SNpc. Scale bar:100 µm (10×), 20 µm (40×). (K) Quantitative analysis of the number of activated microglia in SNpc (cells mm −2 ) (n = 5). (L) The protein level of IL‐1β in the striatum (n = 6). (M) The protein level of IL‐6 in the striatum (n = 6). (N) Pole test (n = 10). (O) Traction test (n = 10). Error bars represent the mean ± SEM. Statistical significance for multiple group comparisons was determined using two‐way ANOVA followed by Tukey's post hoc test. Asterisks (*) indicate significant differences among different treatment groups within the same diet (* p < 0.05, ** p < 0.01, * p < 0.001), while hash symbols (#) indicate significant differences among treatment groups across different diets (# p < 0.05, ## p < 0.01, ### p < 0.001).

Article Snippet: The protein level of IL‐6, and IL‐1β in the striatum ang colon were detected by a commercial mouse IL‐6 ELISA kit (EK0411, Boster, China), and IL‐1β ELISA kit (DY401, R&D, USA).

Techniques: Western Blot, Expressing, Concentration Assay, Staining, Marker

Journal: Advanced Science

Article Title: Neuroprotective Effects of Time‐Restricted Feeding Combined With Different Protein Sources in MPTP‐Induced Parkinson's Disease Mice Model and Its Modulatory Impact on Gut Microbiota Metabolism

doi: 10.1002/advs.202516502

Figure Lengend Snippet: MPTP induces intestinal barrier impairment but not inflammation in animal‐derived casein‐fed mice. (A) Representative IF images of ZO‐1 (red), Occludin (green) and Claudin‐1 (red) in the colon. Scale bar:100 µm (10×), 20 µm (40×). (B–D) Quantification of the mean fluorescence intensity of ZO‐1, Occludin and Claudin‐1 in the colon (n = 5). (E) Representative western blotting of ZO‐1, Occludin and Claudin‐1 proteins in the colon. (F–H) Quantification of ZO‐1, Occludin and Claudin‐1 protein expression in the colon (n = 6). (I) The protein level of IL‐6 in the colon (n = 5). (J) The protein level of IL‐1β in the colon (n = 6). Error bars represent the mean ± SEM. Statistical significance for multiple group comparisons was determined using two‐way ANOVA followed by Tukey's post hoc test. Asterisks (*) indicate significant differences among different treatment groups within the same diet (* p < 0.05, ** p < 0.01, * p < 0.001), while hash symbols (#) indicate significant differences among treatment groups across different diets (# p < 0.05, ## p < 0.01, ### p < 0.001).

Article Snippet: The protein level of IL‐6, and IL‐1β in the striatum ang colon were detected by a commercial mouse IL‐6 ELISA kit (EK0411, Boster, China), and IL‐1β ELISA kit (DY401, R&D, USA).

Techniques: Derivative Assay, Fluorescence, Western Blot, Expressing

Journal: Advanced Science

Article Title: Neuroprotective Effects of Time‐Restricted Feeding Combined With Different Protein Sources in MPTP‐Induced Parkinson's Disease Mice Model and Its Modulatory Impact on Gut Microbiota Metabolism

doi: 10.1002/advs.202516502

Figure Lengend Snippet: TRF suppresses neuroinflammation in PD mice independently of dietary protein source. (A) Double IF staining for TH (green) and GFAP (red) in the SNpc. Scale bar: 100 µm (10×), 20 µm (40×). (B) Quantification of activated astrocytesin the SNpc (cells mm −2 ) (n = 5). (C) Double IF staining for TH and Iba‐1 (red) in the SNpc. Scale bar: 100 µm (10×), 20 µm (40×). (D) Quantification of activated microglia in the SNpc (cells mm −2 ) (n = 5). (E) Protein level of IL‐6 in the striatum (n = 6). (F) Protein level of IL‐1β in the striatum (n = 6). Error bars represent the mean ± SEM. Statistical significance for multiple group comparisons was determined using two‐way ANOVA followed by Tukey's post hoc test. Asterisks (*) indicate significant differences among different treatment groups within the same diet (* p < 0.05, ** p < 0.01, * p < 0.001), while hash symbols (#) indicate significant differences among treatment groups across different diets (# p < 0.05, ## p < 0.01, ### p < 0.001).

Article Snippet: The protein level of IL‐6, and IL‐1β in the striatum ang colon were detected by a commercial mouse IL‐6 ELISA kit (EK0411, Boster, China), and IL‐1β ELISA kit (DY401, R&D, USA).

Techniques: Staining

Journal: Advanced Science

Article Title: Neuroprotective Effects of Time‐Restricted Feeding Combined With Different Protein Sources in MPTP‐Induced Parkinson's Disease Mice Model and Its Modulatory Impact on Gut Microbiota Metabolism

doi: 10.1002/advs.202516502

Figure Lengend Snippet: Excessive BCAAs aggravates neuroinflammation through the Akt/STAT3/NF‐κB pathway in vitro. (A) IL‐6 concentration in BV2 cells (pg mL −1 ) (n = 3). (B) IL‐1β concentration in BV2 cells (pg mL −1 ) (n = 3). (C) Representative western blotting of p‐NF‐κB and NF‐κB in BV2 cells. (D) Quantification of p‐NF‐κB/NF‐κB in BV2 cells (n = 6). (E) Representative western blotting of p‐STAT3 and STAT3 in BV2 cells. (F) Quantification of p‐STAT3/STAT3 in BV2 cells (n = 6). (G) Representative western blotting of p‐Akt and Akt in BV2 cells. (H) Quantification of p‐Akt/Akt in t BV2 cells (n = 6). (I) The mRNA level of Il‐6 in SH‐SY5Y cells (n = 6). (J) The mRNA level of Il‐1β in SH‐SY5Y cells (n = 6). (K) Representative western blotting of p‐NF‐κB and NF‐κB in SH‐SY5Y cells. (L) Quantification of p‐NF‐κB/NF‐κB in SH‐SY5Y cells (n = 6). (M) Representative western blotting of p‐STAT3 and STAT3 in SH‐SY5Y cells. (N) Quantification of p‐STAT3/STAT3 in SH‐SY5Y cells (n = 6). (O) Representative western blotting of p‐Akt and Akt in the SH‐SY5Y cell. (P) Quantification of p‐Akt/Akt in the SH‐SY5Y cells (n = 6). Error bars represent the mean ± SEM. Statistical significance for multiple group comparisons was determined using one‐way ANOVA followed by Tukey's post hoc test. Asterisks (*) indicate significant differences among different groups (* p < 0.05, ** p < 0.01, * p < 0.001).

Article Snippet: The protein level of IL‐6, and IL‐1β in the striatum ang colon were detected by a commercial mouse IL‐6 ELISA kit (EK0411, Boster, China), and IL‐1β ELISA kit (DY401, R&D, USA).

Techniques: In Vitro, Concentration Assay, Western Blot

Journal: BMC Nephrology

Article Title: Malnutrition and inflammation in acute kidney injury due to earthquake-related crush syndrome

doi: 10.1186/1471-2369-11-4

Figure Lengend Snippet: The inflammatory markers in AKI patients treated by RRT and in controls.

Article Snippet: The serum C-reactive protein (CRP), prealbumin, and transferrin levels were measured in participants in Group C and Group E. Interleukin 6 (IL-6) (Wuhan Boster Biological Technology, China) and tumor necrosis factor-α (TNF-α) (Wuhan Boster Biological Technology, China) levels were tested by enzyme-linked immunosorbent assay (ELISA) methods in accordance with the specifications of the manufacturers in Group E and in 18 participants from Group C who received RRT and were followed for more than 14 days.

Techniques: