Journal:

Article Title: In vivo B 1 kinin-receptor upregulation. Evidence for involvement of protein kinases and nuclear factor ?B pathways

doi: 10.1038/sj.bjp.0702715

Figure Lengend Snippet: Effect of co-injection of the recombinant human IL-1 antagonist (IRA, A and B) or of IL-10 (C and D) on des-Arg9-BK-induced paw oedema in rats pre-treated with IL-1β (5 ng paw−1, 60 min beforehand; (A and C)) or with TNFα (5 ng paw−1, 30 min beforehand; (B and D)). Values represent the differences between volumes (in ml) of vehicle-injected (0.1 ml of PBS solution) and drug-injected paws. Each point represents the mean±s.e.mean of 4–6 rats. In some cases the error bars are hidden within the symbols. Significantly different from control values *P<0.05; **P<0.01 (A–C, Student's unpaired t-test) (D, ANOVA followed by Dunnett's test).

Article Snippet: Recombinant murine cytokines IL-1β (Lot BN091), TNFα (Lot CS184) and IL-10 (Lot BC110); anti-murine neutralizing antibodies anti-IL-1β (Lot B01D3), anti-TNFα (Lot CT101), anti-IL-6 (Lot BF 123), anti-IL-8 (Lot BB282) and human recombinant interleukin receptor antagonist (IRA) were obtained from R&D Systems Inc., Minneapolis, U.S.A.

Techniques: Injection, Recombinant, Control

Journal: PLoS ONE

Article Title: Treg/IL-17 Ratio and Treg Differentiation in Patients with COPD

doi: 10.1371/journal.pone.0111044

Figure Lengend Snippet: The original values for the selected cytokines were quantitated in serum obtained from healthy nonsmokers (n = 20) and subjects with SCOPD (n = 23) and AECOPD (n = 21) by ELISA (A). To eliminate the mixed effects of inflammatory factors, the authors divided each original value by the arithmetic mean of a classical inflammatory marker, such as TNF-α (B) and IL-17 (C). The data are presented as the mean ± SEM, unless otherwise stated. *p<0.05 and **p<0.01.

Article Snippet: The concentrations of cytokines (TGF-β, IL-10, TNF-α, IL-17 and IL-9) in serum were measured using ELISA kits according to the manufacturer’s protocols (all kits were purchased from Boster Biosciences Co., Wuhan, China).

Techniques: Enzyme-linked Immunosorbent Assay, Marker

Journal: Central-European journal of immunology

Article Title: Targeting the serum marker interleukin 9 improves the underlying characterization and immune homeostasis in rheumatoid arthritis.

doi: 10.5114/ceji.2024.141695

Figure Lengend Snippet: Fig. 3. Interleukin 9 affected the severity of arthritis in CIA rats. A) ELISA for the detection of IL-9 levels. B) Rat arthritis score. C) Analysis of cartilage damage by saffron O-solid green staining. The descriptive statistics are pre- sented as mean ±SD. *p < 0.05 vs. the CIA group, n = 3

Article Snippet: We measured the levels of serum markers using ELISA with a specialized commercial human IL-9 ELISA Kit (CSB-E04642h, Cusabio, Wuhan, China), human Gal1 ELISA Kit (CSB-EL012882h, Cusabio), human anti-CII ELISA Kit (HB3371-Hu, Shanghai Hengyuan Biotechnology Co., Ltd, Shanghai, China), human sSR-A ELISA Kit (HB3370-Hu, Shanghai Hengyuan Biotechnology Co., Ltd), human anti-Sa ELISA Kit (ml382511V), human RF ELISA Kit (ml060678V), human ACPA ELISA Kit (ml233305V), rat IL-9 ELISA Kit (CSB-E07294r, Cusabio), rat Gal1 ELISA Kit (JL25762, Shanghai Jianglai Biotechnology Co., Ltd, Shanghai, China), rat anti-CII ELISA Kit (HB1364-Ra, Shanghai Hengyuan Biotechnology Co., Ltd), rat sSR-A ELISA Kit (HB1363-Ra, Shanghai Hengyuan Biotechnology Co., Ltd), rat anti-Sa ELISA Kit (ml202241V), rat RF ELISA Kit (ml059541V), and rat ACPA ELISA Kit (ml201458V).

Techniques: Enzyme-linked Immunosorbent Assay, Staining

Journal: Central-European journal of immunology

Article Title: Targeting the serum marker interleukin 9 improves the underlying characterization and immune homeostasis in rheumatoid arthritis.

doi: 10.5114/ceji.2024.141695

Figure Lengend Snippet: Fig. 3. Interleukin 9 affected the severity of arthritis in CIA rats. A) ELISA for the detection of IL-9 levels. B) Rat arthritis score. C) Analysis of cartilage damage by saffron O-solid green staining. The descriptive statistics are pre- sented as mean ±SD. *p < 0.05 vs. the CIA group, n = 3

Article Snippet: We measured the levels of serum markers using ELISA with a specialized commercial human IL-9 ELISA Kit (CSB-E04642h, Cusabio, Wuhan, China), human Gal1 ELISA Kit (CSB-EL012882h, Cusabio), human anti-CII ELISA Kit (HB3371-Hu, Shanghai Hengyuan Biotechnology Co., Ltd, Shanghai, China), human sSR-A ELISA Kit (HB3370-Hu, Shanghai Hengyuan Biotechnology Co., Ltd), human anti-Sa ELISA Kit (ml382511V), human RF ELISA Kit (ml060678V), human ACPA ELISA Kit (ml233305V), rat IL-9 ELISA Kit (CSB-E07294r, Cusabio), rat Gal1 ELISA Kit (JL25762, Shanghai Jianglai Biotechnology Co., Ltd, Shanghai, China), rat anti-CII ELISA Kit (HB1364-Ra, Shanghai Hengyuan Biotechnology Co., Ltd), rat sSR-A ELISA Kit (HB1363-Ra, Shanghai Hengyuan Biotechnology Co., Ltd), rat anti-Sa ELISA Kit (ml202241V), rat RF ELISA Kit (ml059541V), and rat ACPA ELISA Kit (ml201458V).

Techniques: Enzyme-linked Immunosorbent Assay, Staining