Journal: JCI Insight

Article Title: Regulation of bile duct epithelial injury by hepatic CD71 + erythroid cells

doi: 10.1172/jci.insight.135751

Figure Lengend Snippet: (A–C) Representative plots and quantification of CD71+Ter119+ cells (A), CD4+ T cells and CD8+ T cells (B), and activation marker NKp46 in CD3–CD49b+ cells and total NK cell counts (C) in livers of RRV-infected mice pretreated with either rat IgG or anti-CD71 antibody. Differences in mean values analyzed by 2-tailed Student’s t test; **P < 0.01; ****P < 0.0001; n = 4/group.

Article Snippet: In these assays, 4 × 10 5 of responder cells were seeded into 96-well round-bottom plates individually or together with suppressor cells (neonatal hepatic mononuclear cells) at a 1:1 ratio and then stimulated for 72 hours with 0.125 μg/mL anti–mouse CD3 antibody (clone 145-2C11, BioXcell).

Techniques: Activation Assay, Marker, Infection

Journal: JCI Insight

Article Title: Regulation of bile duct epithelial injury by hepatic CD71 + erythroid cells

doi: 10.1172/jci.insight.135751

Figure Lengend Snippet: Adult hepatic mononuclear cells (MNCs) can produce TNF-α upon anti-CD3 stimulation; therefore, they were used as responder cells. Neonatal liver harvested at 24 hours of life, or 3 days after antibody depletion followed by RRV infection, was used as erythroblast pool to function as effector cells. TNF-α levels were measured in supernatant 72 hours after coculture. “+” denotes presence. “–” denotes absence. One-way ANOVA test was performed. *P < 0.05, ***P < 0.001. n = 3/group.

Article Snippet: In these assays, 4 × 10 5 of responder cells were seeded into 96-well round-bottom plates individually or together with suppressor cells (neonatal hepatic mononuclear cells) at a 1:1 ratio and then stimulated for 72 hours with 0.125 μg/mL anti–mouse CD3 antibody (clone 145-2C11, BioXcell).

Techniques: Infection