Journal: Annals of Diagnostic Pathology

Article Title: The histologic and molecular correlates of liver disease in fatal COVID-19 including with alcohol use disorder ☆

doi: 10.1016/j.anndiagpath.2021.151881

Figure Lengend Snippet: Probes and antibodies used for the detection of SARS-CoV2 RNA and proteins and the concomitant host response.

Article Snippet: SARS-COV2 envelope Ab , ProSci/3521 , 1:800/AR.

Techniques:

Journal: Annals of Diagnostic Pathology

Article Title: The histologic and molecular correlates of liver disease in fatal COVID-19 including with alcohol use disorder ☆

doi: 10.1016/j.anndiagpath.2021.151881

Figure Lengend Snippet: Histologic findings and detection of viral RNA/proteins in the liver in fatal COVID-19. Panels A/B show at low and high magnification, respectively, features typical of AUD in the liver/COVID-19 with a hematoxylin and eosin stain. Note the macrosteatosis, abundant Mallory bodies (rectangle) and hepatic stellate cells (oval). In comparison, panel C shows the histologic findings in a NASH/COVID-19 liver where steatosis without Mallory bodies is evident. Panel D shows the histology of another COVID-19 liver without NASH/AUD where lobular inflammation is evident (rectangle). Panel E shows the high copy number SARS-CoV2 RNA in the lung (brown signal); note that the liver from the same person showed only 1 viral RNA positive cell was seen in the entire section (arrow, panel F) (panel G, a viral negative COVID-19/non AUD liver). Panel H shows a Western blot of the lung protein extracts in which the COVID-19 cases show one to several bands when analyzed for the spike S1 and S2 subunit. Panel I: Only the COVID-19 lung samples, and not the liver or heart, showed a band of the expected size (50 kDa) when analyzed for the nucleocapsid protein. Panel J: Most of the COVID-19 liver samples showed a band when analyzed for the viral spike protein; samples 1 and 2 showed the strongest bands and were each AUD/COVID-19 cases. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: SARS-COV2 envelope Ab , ProSci/3521 , 1:800/AR.

Techniques: H&E Stain, Comparison, Western Blot