impdh2 Search Results


impdh bioss  (Bioss)
94
Bioss impdh bioss
Impdh Bioss, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human cytomegalovirus cmv promoter  (OriGene)
90
OriGene human cytomegalovirus cmv promoter
Human Cytomegalovirus Cmv Promoter, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant human inosine monophosphate dehydrogenase 2  (R&D Systems)
93
R&D Systems recombinant human inosine monophosphate dehydrogenase 2
Recombinant Human Inosine Monophosphate Dehydrogenase 2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ap secondary antibodies goat anti rabbit  (Proteintech)
95
Proteintech ap secondary antibodies goat anti rabbit
Ap Secondary Antibodies Goat Anti Rabbit, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ap secondary antibodies goat anti rabbit - by Bioz Stars, 2026-06
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myc flag himpdh2 rc202977 plasmid  (OriGene)
90
OriGene myc flag himpdh2 rc202977 plasmid
Myc Flag Himpdh2 Rc202977 Plasmid, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dicer1  (OriGene)
90
OriGene dicer1
Functional assessment of gene variants identified by exonic mutational pipelines. (A) Three gene variants evaluated in study given their low SIFT and high PolyPhen2 scores. Abbreviations: Chr, chromosome; AA, amino acid; Driver, background driver mutation. (B, C) STRUM structure stability prediction for IMPDH2 S280C (B) and PFKFB4 Y366C (C). Protein structure predicted by iTASSER with mutation highlighted in gray image ( right ). (D) Representative immunofluorescense images showing characteristic rod and ring (RR) structures (red, white arrow) in TPC-1 cells transfected with IMPDH2 WT or IMPDH2 S280C . Scale bar, 10 μm. (E) Representative confocal images showing RR structures (white arrows) in SW1736 cells transfected with IMPDH2 WT or IMPDH2 S280C . Scale bar, 10 μm. (F) Western blot analysis of IMPDH2 in TPC-1 and SW1736 cells transfected with vector-only (VO), IMPDH2 WT , or IMPDH2 S280C . (G) Quantification of scratch wound assay in TPC-1 and SW1736 cells transfected with VO, IMPDH2 WT , or IMPDH2 S280C . %wound recovery determined at indicated time point post-recovery (h). Data presented as mean ± SEM (NS, not significant; ** P < 0.01, *** P < 0.001). (H) Relative cell proliferation of TPC-1 and SW1736 cells transfected with IMPDH2 WT or IMPDH2 S280C for 24 hours relative to VO. Results expressed as mean ± SEM (NS, not significant; * P < 0.05, ** P < 0.01). (I) Representative images of cell invasion experiments in TPC-1 cells transfected with wild-type (WT) and indicated variants of IMPDH2, <t>DICER1,</t> and PFKFB4 relative to VO. (J) Quantification of invading TPC-1 and SW1736 cells from assays described in (I) relative to VO. Data presented as mean ± SEM, 1-way ANOVA (* P < 0.05; ** P < 0.01) or unpaired 2-tailed t test ( # P < 0.05; ## P < 0.01).
Dicer1, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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snp impdh2 c 1842928 10  (Thermo Fisher)
92
Thermo Fisher snp impdh2 c 1842928 10
Functional assessment of gene variants identified by exonic mutational pipelines. (A) Three gene variants evaluated in study given their low SIFT and high PolyPhen2 scores. Abbreviations: Chr, chromosome; AA, amino acid; Driver, background driver mutation. (B, C) STRUM structure stability prediction for IMPDH2 S280C (B) and PFKFB4 Y366C (C). Protein structure predicted by iTASSER with mutation highlighted in gray image ( right ). (D) Representative immunofluorescense images showing characteristic rod and ring (RR) structures (red, white arrow) in TPC-1 cells transfected with IMPDH2 WT or IMPDH2 S280C . Scale bar, 10 μm. (E) Representative confocal images showing RR structures (white arrows) in SW1736 cells transfected with IMPDH2 WT or IMPDH2 S280C . Scale bar, 10 μm. (F) Western blot analysis of IMPDH2 in TPC-1 and SW1736 cells transfected with vector-only (VO), IMPDH2 WT , or IMPDH2 S280C . (G) Quantification of scratch wound assay in TPC-1 and SW1736 cells transfected with VO, IMPDH2 WT , or IMPDH2 S280C . %wound recovery determined at indicated time point post-recovery (h). Data presented as mean ± SEM (NS, not significant; ** P < 0.01, *** P < 0.001). (H) Relative cell proliferation of TPC-1 and SW1736 cells transfected with IMPDH2 WT or IMPDH2 S280C for 24 hours relative to VO. Results expressed as mean ± SEM (NS, not significant; * P < 0.05, ** P < 0.01). (I) Representative images of cell invasion experiments in TPC-1 cells transfected with wild-type (WT) and indicated variants of IMPDH2, <t>DICER1,</t> and PFKFB4 relative to VO. (J) Quantification of invading TPC-1 and SW1736 cells from assays described in (I) relative to VO. Data presented as mean ± SEM, 1-way ANOVA (* P < 0.05; ** P < 0.01) or unpaired 2-tailed t test ( # P < 0.05; ## P < 0.01).
Snp Impdh2 C 1842928 10, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp impdh2 hs00168418 m1  (Thermo Fisher)
85
Thermo Fisher gene exp impdh2 hs00168418 m1
Functional assessment of gene variants identified by exonic mutational pipelines. (A) Three gene variants evaluated in study given their low SIFT and high PolyPhen2 scores. Abbreviations: Chr, chromosome; AA, amino acid; Driver, background driver mutation. (B, C) STRUM structure stability prediction for IMPDH2 S280C (B) and PFKFB4 Y366C (C). Protein structure predicted by iTASSER with mutation highlighted in gray image ( right ). (D) Representative immunofluorescense images showing characteristic rod and ring (RR) structures (red, white arrow) in TPC-1 cells transfected with IMPDH2 WT or IMPDH2 S280C . Scale bar, 10 μm. (E) Representative confocal images showing RR structures (white arrows) in SW1736 cells transfected with IMPDH2 WT or IMPDH2 S280C . Scale bar, 10 μm. (F) Western blot analysis of IMPDH2 in TPC-1 and SW1736 cells transfected with vector-only (VO), IMPDH2 WT , or IMPDH2 S280C . (G) Quantification of scratch wound assay in TPC-1 and SW1736 cells transfected with VO, IMPDH2 WT , or IMPDH2 S280C . %wound recovery determined at indicated time point post-recovery (h). Data presented as mean ± SEM (NS, not significant; ** P < 0.01, *** P < 0.001). (H) Relative cell proliferation of TPC-1 and SW1736 cells transfected with IMPDH2 WT or IMPDH2 S280C for 24 hours relative to VO. Results expressed as mean ± SEM (NS, not significant; * P < 0.05, ** P < 0.01). (I) Representative images of cell invasion experiments in TPC-1 cells transfected with wild-type (WT) and indicated variants of IMPDH2, <t>DICER1,</t> and PFKFB4 relative to VO. (J) Quantification of invading TPC-1 and SW1736 cells from assays described in (I) relative to VO. Data presented as mean ± SEM, 1-way ANOVA (* P < 0.05; ** P < 0.01) or unpaired 2-tailed t test ( # P < 0.05; ## P < 0.01).
Gene Exp Impdh2 Hs00168418 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human inosine  (Cusabio)
88
Cusabio human inosine
Functional assessment of gene variants identified by exonic mutational pipelines. (A) Three gene variants evaluated in study given their low SIFT and high PolyPhen2 scores. Abbreviations: Chr, chromosome; AA, amino acid; Driver, background driver mutation. (B, C) STRUM structure stability prediction for IMPDH2 S280C (B) and PFKFB4 Y366C (C). Protein structure predicted by iTASSER with mutation highlighted in gray image ( right ). (D) Representative immunofluorescense images showing characteristic rod and ring (RR) structures (red, white arrow) in TPC-1 cells transfected with IMPDH2 WT or IMPDH2 S280C . Scale bar, 10 μm. (E) Representative confocal images showing RR structures (white arrows) in SW1736 cells transfected with IMPDH2 WT or IMPDH2 S280C . Scale bar, 10 μm. (F) Western blot analysis of IMPDH2 in TPC-1 and SW1736 cells transfected with vector-only (VO), IMPDH2 WT , or IMPDH2 S280C . (G) Quantification of scratch wound assay in TPC-1 and SW1736 cells transfected with VO, IMPDH2 WT , or IMPDH2 S280C . %wound recovery determined at indicated time point post-recovery (h). Data presented as mean ± SEM (NS, not significant; ** P < 0.01, *** P < 0.001). (H) Relative cell proliferation of TPC-1 and SW1736 cells transfected with IMPDH2 WT or IMPDH2 S280C for 24 hours relative to VO. Results expressed as mean ± SEM (NS, not significant; * P < 0.05, ** P < 0.01). (I) Representative images of cell invasion experiments in TPC-1 cells transfected with wild-type (WT) and indicated variants of IMPDH2, <t>DICER1,</t> and PFKFB4 relative to VO. (J) Quantification of invading TPC-1 and SW1736 cells from assays described in (I) relative to VO. Data presented as mean ± SEM, 1-way ANOVA (* P < 0.05; ** P < 0.01) or unpaired 2-tailed t test ( # P < 0.05; ## P < 0.01).
Human Inosine, supplied by Cusabio, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp impdh2 hs01021353 m1  (Thermo Fisher)
94
Thermo Fisher gene exp impdh2 hs01021353 m1
Functional assessment of gene variants identified by exonic mutational pipelines. (A) Three gene variants evaluated in study given their low SIFT and high PolyPhen2 scores. Abbreviations: Chr, chromosome; AA, amino acid; Driver, background driver mutation. (B, C) STRUM structure stability prediction for IMPDH2 S280C (B) and PFKFB4 Y366C (C). Protein structure predicted by iTASSER with mutation highlighted in gray image ( right ). (D) Representative immunofluorescense images showing characteristic rod and ring (RR) structures (red, white arrow) in TPC-1 cells transfected with IMPDH2 WT or IMPDH2 S280C . Scale bar, 10 μm. (E) Representative confocal images showing RR structures (white arrows) in SW1736 cells transfected with IMPDH2 WT or IMPDH2 S280C . Scale bar, 10 μm. (F) Western blot analysis of IMPDH2 in TPC-1 and SW1736 cells transfected with vector-only (VO), IMPDH2 WT , or IMPDH2 S280C . (G) Quantification of scratch wound assay in TPC-1 and SW1736 cells transfected with VO, IMPDH2 WT , or IMPDH2 S280C . %wound recovery determined at indicated time point post-recovery (h). Data presented as mean ± SEM (NS, not significant; ** P < 0.01, *** P < 0.001). (H) Relative cell proliferation of TPC-1 and SW1736 cells transfected with IMPDH2 WT or IMPDH2 S280C for 24 hours relative to VO. Results expressed as mean ± SEM (NS, not significant; * P < 0.05, ** P < 0.01). (I) Representative images of cell invasion experiments in TPC-1 cells transfected with wild-type (WT) and indicated variants of IMPDH2, <t>DICER1,</t> and PFKFB4 relative to VO. (J) Quantification of invading TPC-1 and SW1736 cells from assays described in (I) relative to VO. Data presented as mean ± SEM, 1-way ANOVA (* P < 0.05; ** P < 0.01) or unpaired 2-tailed t test ( # P < 0.05; ## P < 0.01).
Gene Exp Impdh2 Hs01021353 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hpa001400  (Atlas Antibodies)
93
Atlas Antibodies hpa001400
Functional assessment of gene variants identified by exonic mutational pipelines. (A) Three gene variants evaluated in study given their low SIFT and high PolyPhen2 scores. Abbreviations: Chr, chromosome; AA, amino acid; Driver, background driver mutation. (B, C) STRUM structure stability prediction for IMPDH2 S280C (B) and PFKFB4 Y366C (C). Protein structure predicted by iTASSER with mutation highlighted in gray image ( right ). (D) Representative immunofluorescense images showing characteristic rod and ring (RR) structures (red, white arrow) in TPC-1 cells transfected with IMPDH2 WT or IMPDH2 S280C . Scale bar, 10 μm. (E) Representative confocal images showing RR structures (white arrows) in SW1736 cells transfected with IMPDH2 WT or IMPDH2 S280C . Scale bar, 10 μm. (F) Western blot analysis of IMPDH2 in TPC-1 and SW1736 cells transfected with vector-only (VO), IMPDH2 WT , or IMPDH2 S280C . (G) Quantification of scratch wound assay in TPC-1 and SW1736 cells transfected with VO, IMPDH2 WT , or IMPDH2 S280C . %wound recovery determined at indicated time point post-recovery (h). Data presented as mean ± SEM (NS, not significant; ** P < 0.01, *** P < 0.001). (H) Relative cell proliferation of TPC-1 and SW1736 cells transfected with IMPDH2 WT or IMPDH2 S280C for 24 hours relative to VO. Results expressed as mean ± SEM (NS, not significant; * P < 0.05, ** P < 0.01). (I) Representative images of cell invasion experiments in TPC-1 cells transfected with wild-type (WT) and indicated variants of IMPDH2, <t>DICER1,</t> and PFKFB4 relative to VO. (J) Quantification of invading TPC-1 and SW1736 cells from assays described in (I) relative to VO. Data presented as mean ± SEM, 1-way ANOVA (* P < 0.05; ** P < 0.01) or unpaired 2-tailed t test ( # P < 0.05; ## P < 0.01).
Hpa001400, supplied by Atlas Antibodies, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp impdh2 rn01640111 g1  (Thermo Fisher)
94
Thermo Fisher gene exp impdh2 rn01640111 g1
Functional assessment of gene variants identified by exonic mutational pipelines. (A) Three gene variants evaluated in study given their low SIFT and high PolyPhen2 scores. Abbreviations: Chr, chromosome; AA, amino acid; Driver, background driver mutation. (B, C) STRUM structure stability prediction for IMPDH2 S280C (B) and PFKFB4 Y366C (C). Protein structure predicted by iTASSER with mutation highlighted in gray image ( right ). (D) Representative immunofluorescense images showing characteristic rod and ring (RR) structures (red, white arrow) in TPC-1 cells transfected with IMPDH2 WT or IMPDH2 S280C . Scale bar, 10 μm. (E) Representative confocal images showing RR structures (white arrows) in SW1736 cells transfected with IMPDH2 WT or IMPDH2 S280C . Scale bar, 10 μm. (F) Western blot analysis of IMPDH2 in TPC-1 and SW1736 cells transfected with vector-only (VO), IMPDH2 WT , or IMPDH2 S280C . (G) Quantification of scratch wound assay in TPC-1 and SW1736 cells transfected with VO, IMPDH2 WT , or IMPDH2 S280C . %wound recovery determined at indicated time point post-recovery (h). Data presented as mean ± SEM (NS, not significant; ** P < 0.01, *** P < 0.001). (H) Relative cell proliferation of TPC-1 and SW1736 cells transfected with IMPDH2 WT or IMPDH2 S280C for 24 hours relative to VO. Results expressed as mean ± SEM (NS, not significant; * P < 0.05, ** P < 0.01). (I) Representative images of cell invasion experiments in TPC-1 cells transfected with wild-type (WT) and indicated variants of IMPDH2, <t>DICER1,</t> and PFKFB4 relative to VO. (J) Quantification of invading TPC-1 and SW1736 cells from assays described in (I) relative to VO. Data presented as mean ± SEM, 1-way ANOVA (* P < 0.05; ** P < 0.01) or unpaired 2-tailed t test ( # P < 0.05; ## P < 0.01).
Gene Exp Impdh2 Rn01640111 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Functional assessment of gene variants identified by exonic mutational pipelines. (A) Three gene variants evaluated in study given their low SIFT and high PolyPhen2 scores. Abbreviations: Chr, chromosome; AA, amino acid; Driver, background driver mutation. (B, C) STRUM structure stability prediction for IMPDH2 S280C (B) and PFKFB4 Y366C (C). Protein structure predicted by iTASSER with mutation highlighted in gray image ( right ). (D) Representative immunofluorescense images showing characteristic rod and ring (RR) structures (red, white arrow) in TPC-1 cells transfected with IMPDH2 WT or IMPDH2 S280C . Scale bar, 10 μm. (E) Representative confocal images showing RR structures (white arrows) in SW1736 cells transfected with IMPDH2 WT or IMPDH2 S280C . Scale bar, 10 μm. (F) Western blot analysis of IMPDH2 in TPC-1 and SW1736 cells transfected with vector-only (VO), IMPDH2 WT , or IMPDH2 S280C . (G) Quantification of scratch wound assay in TPC-1 and SW1736 cells transfected with VO, IMPDH2 WT , or IMPDH2 S280C . %wound recovery determined at indicated time point post-recovery (h). Data presented as mean ± SEM (NS, not significant; ** P < 0.01, *** P < 0.001). (H) Relative cell proliferation of TPC-1 and SW1736 cells transfected with IMPDH2 WT or IMPDH2 S280C for 24 hours relative to VO. Results expressed as mean ± SEM (NS, not significant; * P < 0.05, ** P < 0.01). (I) Representative images of cell invasion experiments in TPC-1 cells transfected with wild-type (WT) and indicated variants of IMPDH2, DICER1, and PFKFB4 relative to VO. (J) Quantification of invading TPC-1 and SW1736 cells from assays described in (I) relative to VO. Data presented as mean ± SEM, 1-way ANOVA (* P < 0.05; ** P < 0.01) or unpaired 2-tailed t test ( # P < 0.05; ## P < 0.01).

Journal: The Journal of Clinical Endocrinology and Metabolism

Article Title: Recurrence of Papillary Thyroid Cancer: A Systematic Appraisal of Risk Factors

doi: 10.1210/clinem/dgab836

Figure Lengend Snippet: Functional assessment of gene variants identified by exonic mutational pipelines. (A) Three gene variants evaluated in study given their low SIFT and high PolyPhen2 scores. Abbreviations: Chr, chromosome; AA, amino acid; Driver, background driver mutation. (B, C) STRUM structure stability prediction for IMPDH2 S280C (B) and PFKFB4 Y366C (C). Protein structure predicted by iTASSER with mutation highlighted in gray image ( right ). (D) Representative immunofluorescense images showing characteristic rod and ring (RR) structures (red, white arrow) in TPC-1 cells transfected with IMPDH2 WT or IMPDH2 S280C . Scale bar, 10 μm. (E) Representative confocal images showing RR structures (white arrows) in SW1736 cells transfected with IMPDH2 WT or IMPDH2 S280C . Scale bar, 10 μm. (F) Western blot analysis of IMPDH2 in TPC-1 and SW1736 cells transfected with vector-only (VO), IMPDH2 WT , or IMPDH2 S280C . (G) Quantification of scratch wound assay in TPC-1 and SW1736 cells transfected with VO, IMPDH2 WT , or IMPDH2 S280C . %wound recovery determined at indicated time point post-recovery (h). Data presented as mean ± SEM (NS, not significant; ** P < 0.01, *** P < 0.001). (H) Relative cell proliferation of TPC-1 and SW1736 cells transfected with IMPDH2 WT or IMPDH2 S280C for 24 hours relative to VO. Results expressed as mean ± SEM (NS, not significant; * P < 0.05, ** P < 0.01). (I) Representative images of cell invasion experiments in TPC-1 cells transfected with wild-type (WT) and indicated variants of IMPDH2, DICER1, and PFKFB4 relative to VO. (J) Quantification of invading TPC-1 and SW1736 cells from assays described in (I) relative to VO. Data presented as mean ± SEM, 1-way ANOVA (* P < 0.05; ** P < 0.01) or unpaired 2-tailed t test ( # P < 0.05; ## P < 0.01).

Article Snippet: The following plasmids and expression vectors were transfected into the above thyroid cancer cell lines using transfection reagent TransIT-LT1 (LT1) (GeneFlow; Lichfield, UK) at a ratio of 3 μL to 1 μg DNA in Opti-MEM I reduced serum medium (Life Technologies): DICER1 (pCMV-flag; generously supplied by Prof. P Santisteban, the Autonomous University of Madrid, Spain), IMPDH2 (pCMV6-XL5; #SC119585; Origene Technologies; Rockville, MD, USA), PFKFB4 WT (CMV6-XL5; #SC110972; Origene Technologies; Rockville, MD, USA); FN1 (pPM-N-D-C-HA; #PV355032 abm; Vancouver, Canada), MET (CMV3-ORF; #HG10692-UT Sino Biological; Pennsylvania, USA).

Techniques: Functional Assay, Mutagenesis, Transfection, Western Blot, Plasmid Preparation, Scratch Wound Assay Assay