imp β Search Results


primer sets targeting imp β-1  (Qiagen)
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Qiagen primer sets targeting imp β-1
Western blots from CLU209 cells and rat PVN (A) showing the labeling of the <t>neurokinin</t> <t>3</t> <t>receptor</t> <t>(NK3R,</t> sheep anti-NK3R in CLU209, K7 anti-NK3R in rat) and importin β-1 (Imp β-1, KPNB1 labeling for both samples). RT-PCR from the CLU209 and rat PVN (B) also demonstrate that mRNA for NK3R and imp β-1 are expressed in these samples along with vasopressin (VP). The center marker shows the 300 bp, 150 bp and 50 bp bands respectively.
Primer Sets Targeting Imp β 1, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Western blots from CLU209 cells and rat PVN (A) showing the labeling of the neurokinin 3 receptor (NK3R, sheep anti-NK3R in CLU209, K7 anti-NK3R in rat) and importin β-1 (Imp β-1, KPNB1 labeling for both samples). RT-PCR from the CLU209 and rat PVN (B) also demonstrate that mRNA for NK3R and imp β-1 are expressed in these samples along with vasopressin (VP). The center marker shows the 300 bp, 150 bp and 50 bp bands respectively.

Journal:

Article Title: Expression of the nuclear transport protein importin ?-1 and its association with the neurokinin 3 receptor in the rat hypothalamus following acute hyperosmotic challenge

doi: 10.1016/j.neuroscience.2010.08.015

Figure Lengend Snippet: Western blots from CLU209 cells and rat PVN (A) showing the labeling of the neurokinin 3 receptor (NK3R, sheep anti-NK3R in CLU209, K7 anti-NK3R in rat) and importin β-1 (Imp β-1, KPNB1 labeling for both samples). RT-PCR from the CLU209 and rat PVN (B) also demonstrate that mRNA for NK3R and imp β-1 are expressed in these samples along with vasopressin (VP). The center marker shows the 300 bp, 150 bp and 50 bp bands respectively.

Article Snippet: Validated primer sets targeting NK3R (103 bp amplicon), VP (173 bp amplicon) and imp β-1 (91 bp amplicon) were purchased from Geneglobe (Qiagen, Valencia, CA).

Techniques: Western Blot, Labeling, Reverse Transcription Polymerase Chain Reaction, Marker

Western blots following co-immunoprecipitation from control (A) or 2M NaCl challenged (B) rat. The control rat (A) shows that when the neurokinin 3 receptor (NK3R) was not activated or internalized there was no association with importin β-1 (β-1). Conversely when the NK3R was activated by a 2M NaCl challenge (B) it associated with importin β-1. Western blots also show a band at ~15kD that was present only in the co-immunoprecipitation blots. This band was present even in the absence of primary antibodies and is believed to be part of the magna bind beads that fractured during the elution process. Quantification of labeling density (C) shows that there is 24 times more importin β-1 labeling in the 2M NaCl challenged rats compared to the control rats.

Journal:

Article Title: Expression of the nuclear transport protein importin ?-1 and its association with the neurokinin 3 receptor in the rat hypothalamus following acute hyperosmotic challenge

doi: 10.1016/j.neuroscience.2010.08.015

Figure Lengend Snippet: Western blots following co-immunoprecipitation from control (A) or 2M NaCl challenged (B) rat. The control rat (A) shows that when the neurokinin 3 receptor (NK3R) was not activated or internalized there was no association with importin β-1 (β-1). Conversely when the NK3R was activated by a 2M NaCl challenge (B) it associated with importin β-1. Western blots also show a band at ~15kD that was present only in the co-immunoprecipitation blots. This band was present even in the absence of primary antibodies and is believed to be part of the magna bind beads that fractured during the elution process. Quantification of labeling density (C) shows that there is 24 times more importin β-1 labeling in the 2M NaCl challenged rats compared to the control rats.

Article Snippet: Validated primer sets targeting NK3R (103 bp amplicon), VP (173 bp amplicon) and imp β-1 (91 bp amplicon) were purchased from Geneglobe (Qiagen, Valencia, CA).

Techniques: Western Blot, Immunoprecipitation, Control, Labeling

Western blot showing the immunoprecipitation of importin β-1 (β-1) in the first lane. The Western blot also shows that the neurokinin 3 receptor (NK3R, K7 anti-NK3R antibody) was co-immunoprecipitated with importin β-1 following 2M NaCl challenge.

Journal:

Article Title: Expression of the nuclear transport protein importin ?-1 and its association with the neurokinin 3 receptor in the rat hypothalamus following acute hyperosmotic challenge

doi: 10.1016/j.neuroscience.2010.08.015

Figure Lengend Snippet: Western blot showing the immunoprecipitation of importin β-1 (β-1) in the first lane. The Western blot also shows that the neurokinin 3 receptor (NK3R, K7 anti-NK3R antibody) was co-immunoprecipitated with importin β-1 following 2M NaCl challenge.

Article Snippet: Validated primer sets targeting NK3R (103 bp amplicon), VP (173 bp amplicon) and imp β-1 (91 bp amplicon) were purchased from Geneglobe (Qiagen, Valencia, CA).

Techniques: Western Blot, Immunoprecipitation

Quantification of the labeling density of nuclear neurokinin 3 receptor (NK3R) in the CLU209 cells following importin β-1 immuno-neutralization. Lamin B was used as an internal loading control. The decrease in the nuclear presence of the NK3R was dependent on the time of incubation with the importin β-1 antibody.

Journal:

Article Title: Expression of the nuclear transport protein importin ?-1 and its association with the neurokinin 3 receptor in the rat hypothalamus following acute hyperosmotic challenge

doi: 10.1016/j.neuroscience.2010.08.015

Figure Lengend Snippet: Quantification of the labeling density of nuclear neurokinin 3 receptor (NK3R) in the CLU209 cells following importin β-1 immuno-neutralization. Lamin B was used as an internal loading control. The decrease in the nuclear presence of the NK3R was dependent on the time of incubation with the importin β-1 antibody.

Article Snippet: Validated primer sets targeting NK3R (103 bp amplicon), VP (173 bp amplicon) and imp β-1 (91 bp amplicon) were purchased from Geneglobe (Qiagen, Valencia, CA).

Techniques: Labeling, Neutralization, Control, Incubation

Western blots from CLU209 cell homogenate following incubation with the anti importin β-1 antibody. These data show that there was no decrease in the expression levels of neurokinin 3 receptor (NK3R, sheep anti-NK3R antibody) or lamin B following incubation with the importin β-1 antibody. These data also show that there was no activation of caspases-3 or increase in cell death due to incubation with the antibody.

Journal:

Article Title: Expression of the nuclear transport protein importin ?-1 and its association with the neurokinin 3 receptor in the rat hypothalamus following acute hyperosmotic challenge

doi: 10.1016/j.neuroscience.2010.08.015

Figure Lengend Snippet: Western blots from CLU209 cell homogenate following incubation with the anti importin β-1 antibody. These data show that there was no decrease in the expression levels of neurokinin 3 receptor (NK3R, sheep anti-NK3R antibody) or lamin B following incubation with the importin β-1 antibody. These data also show that there was no activation of caspases-3 or increase in cell death due to incubation with the antibody.

Article Snippet: Validated primer sets targeting NK3R (103 bp amplicon), VP (173 bp amplicon) and imp β-1 (91 bp amplicon) were purchased from Geneglobe (Qiagen, Valencia, CA).

Techniques: Western Blot, Incubation, Expressing, Activation Assay