Journal: Frontiers in Oncology

Article Title: miR-1246 in tumor extracellular vesicles promotes metastasis via increased tumor cell adhesion and endothelial cell barrier destruction

doi: 10.3389/fonc.2023.973871

Figure Lengend Snippet: High metastatic tumor EVs induce tumor cell adhesion to the lung and promote lung metastasis. (A) The experimental design of tumor EV and tumor cell injection. Tumor cells were injected after five injections of A375SM-EVs. After 24 h and 40 days, tumor cell signals were analyzed using an in vivo imaging system (IVIS). (B, C) Tumor cell luminescence intensity in the lungs of the control and A375SM-EV injected groups was detected using IVIS Spectrum 24 h after tumor injection (B) . Quantitative analysis of luminescence intensity (Total flux) was shown in (C) (**P = 0.012, Wilcoxon test; n = 5 mice per group). (D) Tumor cell luminescence intensity in the control and A375SM-EV injected groups after 40 days from tumor injection was detected using IVIS Spectrum. Quantitative analysis of luminescence intensity (Total flux) was shown in (E) (**P = 0.037, Wilcoxon test; n = 5 mice per group). (F) Representative H-E staining images of lung metastases in the two groups. Arrows indicate tumor nodules. (G) The number of metastatic nodules in the whole lung tissue was quantified (**P = 0.026, Wilcoxon test; n = 5 mice per group).

Article Snippet: After 24 h, VivoGlo Luciferin, In Vivo Grade (Promega), was intraperitoneally administered, and the tumor cell signals were detected using IVIS Spectrum (Caliper Life Sciences, Hopkinton, MA, USA) to analyze tumor cell adhesion to the lung.

Techniques: Injection, In Vivo Imaging, Control, Quantitative Luminescence, Staining

Journal: Frontiers in Oncology

Article Title: miR-1246 in tumor extracellular vesicles promotes metastasis via increased tumor cell adhesion and endothelial cell barrier destruction

doi: 10.3389/fonc.2023.973871

Figure Lengend Snippet: miR-1246 knockdown of tumor cells reduces lung metastasis. (A) Images of mice, 28 days after subcutaneous injection of Control-miR A375SM and Anti-miR-1246 A375SM. (B) miR-1246 levels in serum EVs from Control-miR A375SM or Anti-miR-1246 A375SM tumor-bearing mice. Sera were collected 28 days after subcutaneous injection of each tumor (**P = 0.037, Wilcoxon test; data are presented as mean ± SD; n = 3 real-time RT-PCR runs, n = 5 mice per group). (C) Tumor volume was assessed using the formula: (width 2 × length)/2 (mm 3 ). Two-sided Student’s t-test. N.S.: not significant. P = 0.42 (D) Tumor cell fluorescence intensity (GFP signal) in the lungs was detected using IVIS Spectrum. Quantitative analysis of fluorescence intensity (Total flux) was shown in (E) (**P = 0.022, Wilcoxon test; n = 5 mice per group).

Article Snippet: After 24 h, VivoGlo Luciferin, In Vivo Grade (Promega), was intraperitoneally administered, and the tumor cell signals were detected using IVIS Spectrum (Caliper Life Sciences, Hopkinton, MA, USA) to analyze tumor cell adhesion to the lung.

Techniques: Knockdown, Injection, Control, Quantitative RT-PCR, Fluorescence