Journal: Cells

Article Title: Adjunct Therapy with T Regulatory Cells Decreases Inflammation and Preserves the Anti-Tumor Activity of CAR T Cells

doi: 10.3390/cells12141880

Figure Lengend Snippet: UCB Tregs do not increase tumor burden in CAR T cell recipients with xenogeneic lymphoma. Raji tumor burden in mice was evaluated by Non-invasive bioluminescence imaging (BLI) with the IVIS Lumina X5 Imaging System. Ventral (upper row) and dorsal (lower row) images are shown for each day of capture. ( A ) Non-invasive BLI showed clear evidence of disease progression in Arm 1: Raji controls and Arm 2: Raji + UCB Treg cell recipients, with no evidence of disease in Arm 3: Raji + CAR T and Arm 4: Raji + CAR T + UCB Treg recipients on day 10, where these mice had to be euthanized by day 19. Green circles represent planned euthanasia, and red crosses represent mice found dead. ( B ) Quantitative analysis of the BLI showed no differences measured in photons/sec/cm 2 /sr for Arm 3: Raji + CAR T vs. Arm 4: Raji + CAR T +Tregs up to day 51 follow-up. ( C ) CAR T and CAR T + UCB Tregs maintain body weight. Mouse weight was measured on a weekly basis. Readings in different arms are shown as a linear graph over time. ( D ) Data are shown as fold change in serial days compared with baseline. Error bars represent SEM; statistical differences compared with the control arm were quantified by two-way ANOVA using GraphPad Prism software (version 9.5.0).

Article Snippet: Raji tumor burden in mice was evaluated by Non-invasive bioluminescence imaging (BLI) with the IVIS Lumina X5 Imaging System (PerkinElmer, Waltham, MA, USA).

Techniques: Imaging, Biomarker Discovery, Control, Software