Journal:

Article Title: Functional Analysis of the Erwinia herbicola tutB Gene and Its Product

doi: 10.1128/JB.184.11.3135-3141.2002

Figure Lengend Snippet: The Tpl contents of various E. herbicola strains. E. herbicola Δ(tpl-tutB)::kan+ cells transformed with pMBO131 (lanes 1 and 2) or pMBO131 carrying either the tpl-tutB gene (pTK932, lanes 3 and 4) or the tpl-tutB(ATG to CTG) gene (pTK1143, lanes 5 and 6) were grown in M63 minimal medium containing 0.02% yeast extract in the presence and absence of 1 mM tyrosine. When OD600 reached 0.5, the cells were harvested from 1 ml of culture and then suspended in 100 μl of cracking buffer (13). After boiling for 5 min, 10 μl of each whole-cell extract was applied and separated on a sodium dodecyl sulfate-12.5% polyacrylamide gel (18). Immunoblotting with anti-Tpl antibodies was performed as described previously (13). The image on X-ray film was analyzed with a Fujifilm ImageGauge program, and the densitometric values were estimated within the linear range.

Article Snippet: The image on X-ray film was analyzed with a Fujifilm ImageGauge program, and the densitometric values were estimated within the linear range.

Techniques: Transformation Assay, Western Blot