image acquisition image processing toolboxes release 2015a Search Results


90
Chemie GmbH angewandte chemie
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Sartorius AG incucyte zoom microscope software 2015a
Late passage astrocytes show reduced mitochondrial membrane potential and ATP production. (A) Astrocytes were conducted for mitochondrial membrane potential by fluorescence staining of TMRM. Representative images were obtained by the IncuCyte ZOOM microscope software <t>2015A</t> (Essen Bioscience). The data were measured by IncuCyte software (Essen Bioscience). The representative graph shows the total TMRM object integrated intensity (RCU x μm 2 /image) and the total TMRM object area (μm 2 /image). Bars represent the mean ± SEM (n=3). **indicates p <0.01, and ***indicates p <0.001 vs. vehicle group. (B) The mitochondrial OCR of astrocytes was experimented using Cell Mito Test Kit and Agilent Seahorse XFe96 Analyzer (Agilent Technologies). The representative graphs were visualized using Wave Desktop 2.6 software (Agilent Technologies). The graphs represent the basal level, spare respiratory capacity, proton leak, and ATP production level. OCRs are mean ± SEM (n=3), normalized to the third measurement point and expressed as % baseline OCR. *indicates p <0.05 and, ***indicates p <0.001 vs. P1 astrocytes.
Incucyte Zoom Microscope Software 2015a, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Johns Hopkins HealthCare the annihilation of nature: human extinction of birds and mammals
Late passage astrocytes show reduced mitochondrial membrane potential and ATP production. (A) Astrocytes were conducted for mitochondrial membrane potential by fluorescence staining of TMRM. Representative images were obtained by the IncuCyte ZOOM microscope software <t>2015A</t> (Essen Bioscience). The data were measured by IncuCyte software (Essen Bioscience). The representative graph shows the total TMRM object integrated intensity (RCU x μm 2 /image) and the total TMRM object area (μm 2 /image). Bars represent the mean ± SEM (n=3). **indicates p <0.01, and ***indicates p <0.001 vs. vehicle group. (B) The mitochondrial OCR of astrocytes was experimented using Cell Mito Test Kit and Agilent Seahorse XFe96 Analyzer (Agilent Technologies). The representative graphs were visualized using Wave Desktop 2.6 software (Agilent Technologies). The graphs represent the basal level, spare respiratory capacity, proton leak, and ATP production level. OCRs are mean ± SEM (n=3), normalized to the third measurement point and expressed as % baseline OCR. *indicates p <0.05 and, ***indicates p <0.001 vs. P1 astrocytes.
The Annihilation Of Nature: Human Extinction Of Birds And Mammals, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sino Biological influenza a h1n1 (a/michigan/45/2015) neuraminidase / na
Late passage astrocytes show reduced mitochondrial membrane potential and ATP production. (A) Astrocytes were conducted for mitochondrial membrane potential by fluorescence staining of TMRM. Representative images were obtained by the IncuCyte ZOOM microscope software <t>2015A</t> (Essen Bioscience). The data were measured by IncuCyte software (Essen Bioscience). The representative graph shows the total TMRM object integrated intensity (RCU x μm 2 /image) and the total TMRM object area (μm 2 /image). Bars represent the mean ± SEM (n=3). **indicates p <0.01, and ***indicates p <0.001 vs. vehicle group. (B) The mitochondrial OCR of astrocytes was experimented using Cell Mito Test Kit and Agilent Seahorse XFe96 Analyzer (Agilent Technologies). The representative graphs were visualized using Wave Desktop 2.6 software (Agilent Technologies). The graphs represent the basal level, spare respiratory capacity, proton leak, and ATP production level. OCRs are mean ± SEM (n=3), normalized to the third measurement point and expressed as % baseline OCR. *indicates p <0.05 and, ***indicates p <0.001 vs. P1 astrocytes.
Influenza A H1n1 (A/Michigan/45/2015) Neuraminidase / Na, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Solvias AG ni-catalyzed reactions
Late passage astrocytes show reduced mitochondrial membrane potential and ATP production. (A) Astrocytes were conducted for mitochondrial membrane potential by fluorescence staining of TMRM. Representative images were obtained by the IncuCyte ZOOM microscope software <t>2015A</t> (Essen Bioscience). The data were measured by IncuCyte software (Essen Bioscience). The representative graph shows the total TMRM object integrated intensity (RCU x μm 2 /image) and the total TMRM object area (μm 2 /image). Bars represent the mean ± SEM (n=3). **indicates p <0.01, and ***indicates p <0.001 vs. vehicle group. (B) The mitochondrial OCR of astrocytes was experimented using Cell Mito Test Kit and Agilent Seahorse XFe96 Analyzer (Agilent Technologies). The representative graphs were visualized using Wave Desktop 2.6 software (Agilent Technologies). The graphs represent the basal level, spare respiratory capacity, proton leak, and ATP production level. OCRs are mean ± SEM (n=3), normalized to the third measurement point and expressed as % baseline OCR. *indicates p <0.05 and, ***indicates p <0.001 vs. P1 astrocytes.
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86
Stryker behavioral responses 2015a
Late passage astrocytes show reduced mitochondrial membrane potential and ATP production. (A) Astrocytes were conducted for mitochondrial membrane potential by fluorescence staining of TMRM. Representative images were obtained by the IncuCyte ZOOM microscope software <t>2015A</t> (Essen Bioscience). The data were measured by IncuCyte software (Essen Bioscience). The representative graph shows the total TMRM object integrated intensity (RCU x μm 2 /image) and the total TMRM object area (μm 2 /image). Bars represent the mean ± SEM (n=3). **indicates p <0.01, and ***indicates p <0.001 vs. vehicle group. (B) The mitochondrial OCR of astrocytes was experimented using Cell Mito Test Kit and Agilent Seahorse XFe96 Analyzer (Agilent Technologies). The representative graphs were visualized using Wave Desktop 2.6 software (Agilent Technologies). The graphs represent the basal level, spare respiratory capacity, proton leak, and ATP production level. OCRs are mean ± SEM (n=3), normalized to the third measurement point and expressed as % baseline OCR. *indicates p <0.05 and, ***indicates p <0.001 vs. P1 astrocytes.
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MathWorks Inc 2015a
Late passage astrocytes show reduced mitochondrial membrane potential and ATP production. (A) Astrocytes were conducted for mitochondrial membrane potential by fluorescence staining of TMRM. Representative images were obtained by the IncuCyte ZOOM microscope software <t>2015A</t> (Essen Bioscience). The data were measured by IncuCyte software (Essen Bioscience). The representative graph shows the total TMRM object integrated intensity (RCU x μm 2 /image) and the total TMRM object area (μm 2 /image). Bars represent the mean ± SEM (n=3). **indicates p <0.01, and ***indicates p <0.001 vs. vehicle group. (B) The mitochondrial OCR of astrocytes was experimented using Cell Mito Test Kit and Agilent Seahorse XFe96 Analyzer (Agilent Technologies). The representative graphs were visualized using Wave Desktop 2.6 software (Agilent Technologies). The graphs represent the basal level, spare respiratory capacity, proton leak, and ATP production level. OCRs are mean ± SEM (n=3), normalized to the third measurement point and expressed as % baseline OCR. *indicates p <0.05 and, ***indicates p <0.001 vs. P1 astrocytes.
2015a, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
IITC Life Science hargreaves' method
Late passage astrocytes show reduced mitochondrial membrane potential and ATP production. (A) Astrocytes were conducted for mitochondrial membrane potential by fluorescence staining of TMRM. Representative images were obtained by the IncuCyte ZOOM microscope software <t>2015A</t> (Essen Bioscience). The data were measured by IncuCyte software (Essen Bioscience). The representative graph shows the total TMRM object integrated intensity (RCU x μm 2 /image) and the total TMRM object area (μm 2 /image). Bars represent the mean ± SEM (n=3). **indicates p <0.01, and ***indicates p <0.001 vs. vehicle group. (B) The mitochondrial OCR of astrocytes was experimented using Cell Mito Test Kit and Agilent Seahorse XFe96 Analyzer (Agilent Technologies). The representative graphs were visualized using Wave Desktop 2.6 software (Agilent Technologies). The graphs represent the basal level, spare respiratory capacity, proton leak, and ATP production level. OCRs are mean ± SEM (n=3), normalized to the third measurement point and expressed as % baseline OCR. *indicates p <0.05 and, ***indicates p <0.001 vs. P1 astrocytes.
Hargreaves' Method, supplied by IITC Life Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Broad Institute Inc crispr-cas9 genome editing
Late passage astrocytes show reduced mitochondrial membrane potential and ATP production. (A) Astrocytes were conducted for mitochondrial membrane potential by fluorescence staining of TMRM. Representative images were obtained by the IncuCyte ZOOM microscope software <t>2015A</t> (Essen Bioscience). The data were measured by IncuCyte software (Essen Bioscience). The representative graph shows the total TMRM object integrated intensity (RCU x μm 2 /image) and the total TMRM object area (μm 2 /image). Bars represent the mean ± SEM (n=3). **indicates p <0.01, and ***indicates p <0.001 vs. vehicle group. (B) The mitochondrial OCR of astrocytes was experimented using Cell Mito Test Kit and Agilent Seahorse XFe96 Analyzer (Agilent Technologies). The representative graphs were visualized using Wave Desktop 2.6 software (Agilent Technologies). The graphs represent the basal level, spare respiratory capacity, proton leak, and ATP production level. OCRs are mean ± SEM (n=3), normalized to the third measurement point and expressed as % baseline OCR. *indicates p <0.05 and, ***indicates p <0.001 vs. P1 astrocytes.
Crispr Cas9 Genome Editing, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Scalex Corporation doppler boundary layer lidars
Late passage astrocytes show reduced mitochondrial membrane potential and ATP production. (A) Astrocytes were conducted for mitochondrial membrane potential by fluorescence staining of TMRM. Representative images were obtained by the IncuCyte ZOOM microscope software <t>2015A</t> (Essen Bioscience). The data were measured by IncuCyte software (Essen Bioscience). The representative graph shows the total TMRM object integrated intensity (RCU x μm 2 /image) and the total TMRM object area (μm 2 /image). Bars represent the mean ± SEM (n=3). **indicates p <0.01, and ***indicates p <0.001 vs. vehicle group. (B) The mitochondrial OCR of astrocytes was experimented using Cell Mito Test Kit and Agilent Seahorse XFe96 Analyzer (Agilent Technologies). The representative graphs were visualized using Wave Desktop 2.6 software (Agilent Technologies). The graphs represent the basal level, spare respiratory capacity, proton leak, and ATP production level. OCRs are mean ± SEM (n=3), normalized to the third measurement point and expressed as % baseline OCR. *indicates p <0.05 and, ***indicates p <0.001 vs. P1 astrocytes.
Doppler Boundary Layer Lidars, supplied by Scalex Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sino Biological influenza a h1n1 (a/michigan/45/2015) nucleoprotein / np protein
Late passage astrocytes show reduced mitochondrial membrane potential and ATP production. (A) Astrocytes were conducted for mitochondrial membrane potential by fluorescence staining of TMRM. Representative images were obtained by the IncuCyte ZOOM microscope software <t>2015A</t> (Essen Bioscience). The data were measured by IncuCyte software (Essen Bioscience). The representative graph shows the total TMRM object integrated intensity (RCU x μm 2 /image) and the total TMRM object area (μm 2 /image). Bars represent the mean ± SEM (n=3). **indicates p <0.01, and ***indicates p <0.001 vs. vehicle group. (B) The mitochondrial OCR of astrocytes was experimented using Cell Mito Test Kit and Agilent Seahorse XFe96 Analyzer (Agilent Technologies). The representative graphs were visualized using Wave Desktop 2.6 software (Agilent Technologies). The graphs represent the basal level, spare respiratory capacity, proton leak, and ATP production level. OCRs are mean ± SEM (n=3), normalized to the third measurement point and expressed as % baseline OCR. *indicates p <0.05 and, ***indicates p <0.001 vs. P1 astrocytes.
Influenza A H1n1 (A/Michigan/45/2015) Nucleoprotein / Np Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Regent Instruments winfolia pro 2015a software
Late passage astrocytes show reduced mitochondrial membrane potential and ATP production. (A) Astrocytes were conducted for mitochondrial membrane potential by fluorescence staining of TMRM. Representative images were obtained by the IncuCyte ZOOM microscope software <t>2015A</t> (Essen Bioscience). The data were measured by IncuCyte software (Essen Bioscience). The representative graph shows the total TMRM object integrated intensity (RCU x μm 2 /image) and the total TMRM object area (μm 2 /image). Bars represent the mean ± SEM (n=3). **indicates p <0.01, and ***indicates p <0.001 vs. vehicle group. (B) The mitochondrial OCR of astrocytes was experimented using Cell Mito Test Kit and Agilent Seahorse XFe96 Analyzer (Agilent Technologies). The representative graphs were visualized using Wave Desktop 2.6 software (Agilent Technologies). The graphs represent the basal level, spare respiratory capacity, proton leak, and ATP production level. OCRs are mean ± SEM (n=3), normalized to the third measurement point and expressed as % baseline OCR. *indicates p <0.05 and, ***indicates p <0.001 vs. P1 astrocytes.
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Image Search Results


Late passage astrocytes show reduced mitochondrial membrane potential and ATP production. (A) Astrocytes were conducted for mitochondrial membrane potential by fluorescence staining of TMRM. Representative images were obtained by the IncuCyte ZOOM microscope software 2015A (Essen Bioscience). The data were measured by IncuCyte software (Essen Bioscience). The representative graph shows the total TMRM object integrated intensity (RCU x μm 2 /image) and the total TMRM object area (μm 2 /image). Bars represent the mean ± SEM (n=3). **indicates p <0.01, and ***indicates p <0.001 vs. vehicle group. (B) The mitochondrial OCR of astrocytes was experimented using Cell Mito Test Kit and Agilent Seahorse XFe96 Analyzer (Agilent Technologies). The representative graphs were visualized using Wave Desktop 2.6 software (Agilent Technologies). The graphs represent the basal level, spare respiratory capacity, proton leak, and ATP production level. OCRs are mean ± SEM (n=3), normalized to the third measurement point and expressed as % baseline OCR. *indicates p <0.05 and, ***indicates p <0.001 vs. P1 astrocytes.

Journal: Biomolecules & Therapeutics

Article Title: Late Passage Cultivation Induces Aged Astrocyte Phenotypes in Rat Primary Cultured Cells

doi: 10.4062/biomolther.2020.175

Figure Lengend Snippet: Late passage astrocytes show reduced mitochondrial membrane potential and ATP production. (A) Astrocytes were conducted for mitochondrial membrane potential by fluorescence staining of TMRM. Representative images were obtained by the IncuCyte ZOOM microscope software 2015A (Essen Bioscience). The data were measured by IncuCyte software (Essen Bioscience). The representative graph shows the total TMRM object integrated intensity (RCU x μm 2 /image) and the total TMRM object area (μm 2 /image). Bars represent the mean ± SEM (n=3). **indicates p <0.01, and ***indicates p <0.001 vs. vehicle group. (B) The mitochondrial OCR of astrocytes was experimented using Cell Mito Test Kit and Agilent Seahorse XFe96 Analyzer (Agilent Technologies). The representative graphs were visualized using Wave Desktop 2.6 software (Agilent Technologies). The graphs represent the basal level, spare respiratory capacity, proton leak, and ATP production level. OCRs are mean ± SEM (n=3), normalized to the third measurement point and expressed as % baseline OCR. *indicates p <0.05 and, ***indicates p <0.001 vs. P1 astrocytes.

Article Snippet: We detected the red fluorescence using the IncuCyte ZOOM system (Essen Bioscience) by imaging each well every 30 min for 12 h and analyzed the red particles using the IncuCyte ZOOM microscope software 2015A (Essen Bioscience).

Techniques: Membrane, Fluorescence, Staining, Microscopy, Software