Journal: Discover Oncology

Article Title: The prognostic value of miR-323b-5p in non-small cell lung cancer and its mechanism of targeting IL1A in regulating the proliferation and cell cycle of non-small cell lung cancer cells

doi: 10.1007/s12672-026-04409-2

Figure Lengend Snippet: miR-323b-5p targets IL1A. A The intersection of predicted targets from TargetScan and miRDB databases. B The expression of IL1A and MEOX2 in A549, HCC827, NCI-H1299 and BEAS-2B cells. C The expression of IL1A in the serum of NSCLC patients ( n = 120) and healthy subjects ( n = 60). D The binding site of miR-323b-5p and IL1A. E Dual-luciferase reporter assay indicates that miR-323b-5p directly regulates IL1A. F Expression of IL1A in A549, HCC827, and NCI-H1299 cells after transfection with miR-323b-5p mimics. The experiment was repeated 3 times independently. ( p < 0.05*, p < 0.001***)

Article Snippet: The antibodies used include: IL1A (Wuhan Sanying, 1:1000), P21 (Wuhan Sanying, 1:1500), CCND1 (Wuhan Sanying, 1:5000), cyclin dependent kinase 4 (CDK4, Wuhan Sanying, 1: 1000), β-actin (Wuhan Sanying, 1:2000).

Techniques: Expressing, Binding Assay, Luciferase, Reporter Assay, Transfection

Journal: Discover Oncology

Article Title: The prognostic value of miR-323b-5p in non-small cell lung cancer and its mechanism of targeting IL1A in regulating the proliferation and cell cycle of non-small cell lung cancer cells

doi: 10.1007/s12672-026-04409-2

Figure Lengend Snippet: miR-323b-5p affects A549 cell proliferation and blocks the cell cycle through IL1A. After transfection or non-transfection of A549 cells with the control mimic (mimic NC), miR-323b-5p mimic (mimic MIR), IL1A overexpression plasmid (ovIL1A), control inhibitor (inhibitor NC), miR-323b-5p inhibitor (inhibitor MIR) and IL1A knockdown plasmid (siIL1A). The transfection efficiency of each group of cells was detected by RT-qPCR ( A ), cell proliferation was detected by CCK-8 ( B ), the cell cycle status of A549 cells was detected by flow cytometry ( C ), and the expression of P21, CCND1 and CDK4 mRNA ( D ) and protein ( E ) in the cells was detected by RT-qPCR and WB respectively. The experiment was repeated 3 times independently with 3 biological replicates ( n = 3). (*compared with Control, p < 0.05*. # compared with mimic NC, p < 0.05 # .)

Article Snippet: The antibodies used include: IL1A (Wuhan Sanying, 1:1000), P21 (Wuhan Sanying, 1:1500), CCND1 (Wuhan Sanying, 1:5000), cyclin dependent kinase 4 (CDK4, Wuhan Sanying, 1: 1000), β-actin (Wuhan Sanying, 1:2000).

Techniques: Transfection, Control, Over Expression, Plasmid Preparation, Knockdown, Quantitative RT-PCR, CCK-8 Assay, Flow Cytometry, Expressing

Journal: Discover Oncology

Article Title: The prognostic value of miR-323b-5p in non-small cell lung cancer and its mechanism of targeting IL1A in regulating the proliferation and cell cycle of non-small cell lung cancer cells

doi: 10.1007/s12672-026-04409-2

Figure Lengend Snippet: miR-323b-5p affects HCC827 cell proliferation and blocks the cell cycle through IL1A. After transfection or non-transfection of A549 cells with the control mimic (mimic NC), miR-323b-5p mimic (mimic MIR), IL1A overexpression plasmid (ovIL1A), control inhibitor (inhibitor NC), miR-323b-5p inhibitor (inhibitor MIR) and IL1A knockdown plasmid (siIL1A). The transfection efficiency of each group of cells was detected by RT-qPCR ( A ), cell proliferation was detected by CCK-8 ( B ), the cell cycle status of A549 cells was detected by flow cytometry ( C ), and the expression of P21, CCND1 and CDK4 mRNA ( D ) and protein ( E ) in the cells was detected by RT-qPCR and WB respectively. The experiment was repeated 3 times independently with 3 biological replicates ( n = 3). (*compared with Control, p < 0.001***. # compared with mimic NC, p < 0.001 ### . & compared with inhibitor NC, p < 0.05 & )

Article Snippet: The antibodies used include: IL1A (Wuhan Sanying, 1:1000), P21 (Wuhan Sanying, 1:1500), CCND1 (Wuhan Sanying, 1:5000), cyclin dependent kinase 4 (CDK4, Wuhan Sanying, 1: 1000), β-actin (Wuhan Sanying, 1:2000).

Techniques: Transfection, Control, Over Expression, Plasmid Preparation, Knockdown, Quantitative RT-PCR, CCK-8 Assay, Flow Cytometry, Expressing

Journal: Discover Oncology

Article Title: The prognostic value of miR-323b-5p in non-small cell lung cancer and its mechanism of targeting IL1A in regulating the proliferation and cell cycle of non-small cell lung cancer cells

doi: 10.1007/s12672-026-04409-2

Figure Lengend Snippet: miR-323b-5p affects NCI-H1299 cell proliferation and blocks the cell cycle through IL1A. After transfection or non-transfection of A549 cells with the control mimic (mimic NC), miR-323b-5p mimic (mimic MIR), IL1A overexpression plasmid (ovIL1A), control inhibitor (inhibitor NC), miR-323b-5p inhibitor (inhibitor MIR) and IL1A knockdown plasmid (siIL1A). The transfection efficiency of each group of cells was detected by RT-qPCR ( A ), cell proliferation was detected by CCK-8 ( B ), the cell cycle status of A549 cells was detected by flow cytometry ( C ), and the expression of P21, CCND1 and CDK4 mRNA ( D ) and protein ( E ) in the cells was detected by RT-qPCR and WB respectively. The experiment was repeated 3 times independently with 3 biological replicates ( n = 3). ( # compared with mimic NC, p < 0.001 ### .)

Article Snippet: The antibodies used include: IL1A (Wuhan Sanying, 1:1000), P21 (Wuhan Sanying, 1:1500), CCND1 (Wuhan Sanying, 1:5000), cyclin dependent kinase 4 (CDK4, Wuhan Sanying, 1: 1000), β-actin (Wuhan Sanying, 1:2000).

Techniques: Transfection, Control, Over Expression, Plasmid Preparation, Knockdown, Quantitative RT-PCR, CCK-8 Assay, Flow Cytometry, Expressing

Journal: Journal of Translational Medicine

Article Title: MicroRNA-regulated pathways of flow-stimulated angiogenesis and vascular remodeling in vivo

doi: 10.1186/s12967-019-1767-9

Figure Lengend Snippet: Inverse correlations between micro-RNAs and up-regulated messenger RNAs (mRNA) which induce angiogenesis as well as down-regulated mRNAs which inhibit angiogenesis

Article Snippet: Serial sections of 4 μm thickness were generated and immunostained for C–X–C motif chemokine ligand 2 (CXCL2) (unconjugated rabbit polyclonal anti-CXCL2 antibody, LS-C-415005-100, LifeSpan BioSciences, Seattle, WA, USA) and IL1A (anti-IL1A primary antibody, OAAN00770, Aviva Systems Biology, San Diego, CA, USA).

Techniques:

Journal: Journal of Translational Medicine

Article Title: MicroRNA-regulated pathways of flow-stimulated angiogenesis and vascular remodeling in vivo

doi: 10.1186/s12967-019-1767-9

Figure Lengend Snippet: a Inverse correlations of microRNAs and messenger RNAs with relevance to angiogenesis. Deregulated microRNAs (miRNAs) and messenger RNAs (mRNAs) with significant inverse correlations as well as association with angiogenesis-related gene ontology (GO) terms and predicted target interactions according to TargetScan. Green background: up-regulated mRNAs. Red background: down-regulated mRNAs. Connections between miRNAs and mRNAs represent significant inverse correlations (r < − 0.5, P < 5 × 10 −5 ). b Schematic workflow of the study for identification of miRNA-regulated pathways in flow-stimulated angiogenesis. Spearman correlation plots show significant inverse correlations between the expression levels of synaptojanin-2 binding protein (SYNJ2BP) and miR-449-5p ( c ) as well as miR 223-3p ( d ), ephrin receptor kinase 2 (EPHA2) and miR-223-3p ( e ), forkhead box C1 (FOXC1) and miR-511-3p ( f ), interleukin-1 alpha (IL1A) and miR-27b-3p ( g ), as well as C–X–C chemokine receptor 2 and let-7b-5p ( h )

Article Snippet: Serial sections of 4 μm thickness were generated and immunostained for C–X–C motif chemokine ligand 2 (CXCL2) (unconjugated rabbit polyclonal anti-CXCL2 antibody, LS-C-415005-100, LifeSpan BioSciences, Seattle, WA, USA) and IL1A (anti-IL1A primary antibody, OAAN00770, Aviva Systems Biology, San Diego, CA, USA).

Techniques: Expressing, Binding Assay

Journal: Journal of Translational Medicine

Article Title: MicroRNA-regulated pathways of flow-stimulated angiogenesis and vascular remodeling in vivo

doi: 10.1186/s12967-019-1767-9

Figure Lengend Snippet: Immunohistochemical analysis of C–X–C chemokine ligand 2 (CXCL2) and interleukin-1 alpha (IL1A) protein expression in AV shunt and control vein (Co) cross sections

Article Snippet: Serial sections of 4 μm thickness were generated and immunostained for C–X–C motif chemokine ligand 2 (CXCL2) (unconjugated rabbit polyclonal anti-CXCL2 antibody, LS-C-415005-100, LifeSpan BioSciences, Seattle, WA, USA) and IL1A (anti-IL1A primary antibody, OAAN00770, Aviva Systems Biology, San Diego, CA, USA).

Techniques: Immunohistochemical staining, Expressing, Control

Journal: Journal of Translational Medicine

Article Title: MicroRNA-regulated pathways of flow-stimulated angiogenesis and vascular remodeling in vivo

doi: 10.1186/s12967-019-1767-9

Figure Lengend Snippet: Immunohistochemical staining for CXCL2 and IL1A. Immunohistochemical analysis of C–X–C chemokine ligand 2 (CXCL2) ( a – c ) and interleukin-1 alpha (IL1A) protein expression ( d – f ) in AV shunt and control vein (Co) cross sections show a significant increase in the expression of both proteins in the endothelial cells of AV shunts on postoperative day (POD) 5 ( a , d ) and an even stronger expression on POD 15 ( c , f ) compared to control veins ( a , d ). The black Indian ink filling the vessel lumen (*) appears detached from the endothelium in some sections due to cutting artifacts

Article Snippet: Serial sections of 4 μm thickness were generated and immunostained for C–X–C motif chemokine ligand 2 (CXCL2) (unconjugated rabbit polyclonal anti-CXCL2 antibody, LS-C-415005-100, LifeSpan BioSciences, Seattle, WA, USA) and IL1A (anti-IL1A primary antibody, OAAN00770, Aviva Systems Biology, San Diego, CA, USA).

Techniques: Immunohistochemical staining, Staining, Expressing, Control

Journal: Journal of Cosmetic Dermatology

Article Title: Protective Effects of Exogenous Donkey Oil on Skin Healing Under Incisional Wound Damage

doi: 10.1111/jocd.70550

Figure Lengend Snippet: Effects of different concentrations of DO on the content of VEGF (A) and MMP‐9 (B) in the skin tissue of mice after incision injury. # p < 0.05, ### p < 0.001 vs. BC group; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. NC group.

Article Snippet: The ELISA kits IL‐1α and VEGF were purchased from ProteinTech (Wuhan, China, Cat No: KE10024 and KE10009); IL‐6 from Multi Sciences (Hangzhou, China, Cat No: EK206/3‐96); PGE2 from Lanpaibio (Shanghai, China, Cat No: LP‐M05161); MMP‐9 from CUSABIO (Wuhan, China, Cat No: CSB‐E08007m).

Techniques: