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Image Search Results
Journal: Frontiers in Immunology
Article Title: NOD-Like Receptor P3 Inflammasome Controls Protective Th1/Th17 Immunity against Pulmonary Paracoccidioidomycosis
doi: 10.3389/fimmu.2017.00786
Figure Lengend Snippet: Paracoccidioides brasiliensis infection induces an increased expression of mRNA from NLRP3 inflammasome-related components, which is abolished in Nlrp3 −/− , Casp1/11 −/− , and Asc −/− mice. Real-time quantitative polymerase chain reaction analysis of the IL-1β, IL-18, Asc, Casp-1, Nlrp3, and Syk genes expression in the lung macerates of Nlrp3 −/− , Casp1/11 −/− , Asc −/− , and WT uninfected mice (A) and at week 4 of infection (B) .
Article Snippet: The primers used were: IL-1β Mm01336189_m1; IL-18
Techniques: Infection, Expressing, Real-time Polymerase Chain Reaction
Journal: Frontiers in Immunology
Article Title: NOD-Like Receptor P3 Inflammasome Controls Protective Th1/Th17 Immunity against Pulmonary Paracoccidioidomycosis
doi: 10.3389/fimmu.2017.00786
Figure Lengend Snippet: Secretion of IL-1β and IL-18 but not TNF-α and IL-6 is controlled by NLRP3 inflammasome activation. The supernatants of macerated lungs from WT, Nlrp3 −/− , Casp1 −/− P2x7r −/− , and Asc −/− mice infected via i.t. with 1 × 10 6 Paracoccidioides brasiliensis yeasts were used to assess IL-1β, IL-18, TNF-α and IL-6 production by ELISA. Levels of IL-1β and IL-18 (A,B) and TNF-α and IL-6 (C,D) in the lungs of mice 48 h, 2, 4, and 10 weeks after infection with P. brasiliensis yeasts. Data represents one of two independent experiments using five mice per group ( n = 5). Experiment was performed in triplicates represented as Mean ± SEM (** P < 0.01 and *** P < 0.001 compared with deficient mice).
Article Snippet: The primers used were: IL-1β Mm01336189_m1; IL-18
Techniques: Activation Assay, Infection, Enzyme-linked Immunosorbent Assay