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Image Search Results
Journal: Inflammation and Regeneration
Article Title: Novel artificial nerve transplantation of human iPSC-derived neurite bundles enhanced nerve regeneration after peripheral nerve injury
doi: 10.1186/s41232-024-00319-4
Figure Lengend Snippet: Early-stage tissue evaluation after neurite bundle-derived artificial nerve transplantation. a , b Immunohistochemistry images with anti-NFH and anti-CD31 antibodies. Dotted arrows indicate the proximal side of the intact nerve areas, and solid arrows indicate the NFH- and CD31-positive regeneration tip in the reconstructed tissue. Scale bars = 1 mm. c , d Quantitative comparison of axonal extension distance was indicated by NFH-positive areas and that of vascular elongation distance was demonstrated by CD31-positive areas at 1 and 2 weeks after transplantation. Significant differences between groups elongation distances were observed in 2-week samples only. e , f Iba1 immunohistochemistry image of the proximal part of the regenerated tissue at 2 weeks after transplantation (e). Scale bars = 1 mm. Quantitative analysis of Iba1-positive areas demonstrated that a larger number of macrophages aggregated in the Auto, motor TP and sensory TP (f). ( n = 3). * p < 0.05, ** p < 0.01, N.S. = not significant. Data are represented as the mean ± SEM
Article Snippet: The primary antibodies used in this study were human polyclonal anti-pan-ELAVL (ELAV-like protein 2/3/4) antibody (1:2000, kindly provided from Prof. Robert Darnell, Rockefeller University), rabbit polyclonal anti-TrkA antibody (1:500, kindly provided from Prof. Louis F. Reichardt, UCSF), chicken polyclonal anti-TrkB antibody (1:500, kindly provided from Prof. Louis F. Reichardt, UCSF), goat polyclonal anti-TrkC antibody (AF373, 1:200, R&D systems), rabbit polyclonal anti-parvalbumin antibody (PV-28, 1:1000, Swant), rabbit polyclonal anti-CGRP antibody (BML-CA1134, 1:500, Enzo Life Sciences), Goat polyclonal anti-Choline acetyltransferase antibody (AB144P, 1:200, Chemicon), Mouse monoclonal anti-Islet1 and Islet2 antibody (39.4D5, 1:200, DSHB), rabbit polyclonal anti-Neurofilament heavy polypeptide antibody (ab8135, 1:500, Abcam), goat polyclonal anti-mouse and anti-rat CD31 (AF3628, 1:100, R&D),
Techniques: Derivative Assay, Transplantation Assay, Immunohistochemistry, Comparison
Journal: Cancers
Article Title: Inhibition of the Growth of Breast Cancer-Associated Brain Tumors by the Osteocyte-Derived Conditioned Medium
doi: 10.3390/cancers13051061
Figure Lengend Snippet: Anti-tumor effects of IL1ra overexpression in osteocytes. Of note, CN = control, and CM = conditioned medium. The single and double asterisks indicate p < 0.05 and p < 0.01, respectively. The error bar indicates a standard deviation. ( A ) Overexpression of IL1ra in osteocytes by the transfection of IL1ra plasmids. ( B – D ) Reduction in MTT-based cellular viability, scratch-based migration, and Transwell invasion in EO771 cells by IL1ra-overexpressing CM. ( E ) Reduction in the size and weight of mammary tumors in C57BL/6 mice ( N = 8–12 per group) by the co-injection of IL1ra-overexpressing osteocytes. ( F ) Suppressive effects of osteocytes, overexpressed with IL-1ra, and/or Lrp5 in C57BL/6 mice. Whole brains in the three groups (placebo, injection of IL1ra-overexpressing osteocytes, and injection of IL1ra/Lrp5-overexpressing osteocytes; N = 8 per group). ( G ) Histological analysis, showing that the osteocyte injection significantly suppressed tumor growth, and the overexpression of IL1ra and Lrp5 further reduced tumor progression.
Article Snippet: For the spheroid assay, 1.0 × 10 4 cells/well were cultured in a U-bottom low-adhesion 96-well plate (S-Bio, Hudson, NH, USA) for 24 h. We transfected Lrp5 plasmids (#115907, Addgene, Watertown, MA, USA), β-catenin plasmids (#31785, Addgene), and
Techniques: Over Expression, Control, Standard Deviation, Transfection, Migration, Injection