il-6 Search Results


mab2061  (R&D Systems)
94
R&D Systems mab2061
Mab2061, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti il 6 antibody  (R&D Systems)
99
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il 6 duoset elisa  (R&D Systems)
99
R&D Systems il 6 duoset elisa
a , b A549 epithelial cells or macrophages were infected with the wild-type (WT), the nctA null mutant ( ∆nctA ) or the nctA reconstituted isolate ( nctA rec ) for 24 h. Cytotoxicity of each mutant was evaluated by measuring the release of lactate dehydrogenase (LDH) activity into the culture medium. The data represent five different infection challenges with triplicate LDH activity measurements. Data are shown in fold change of LDH activity relative to the wild-type-infected cells. The error bars mean the standard error of the mean (SEM), and P- values were calculated by Kruskal–Wallis test with Dunn’s correction: * P < 0.0180; ** P < 0.0056 (for a , A549 cells). ** P < 0.0032; *** P < 0.0007 (for b , macrophages). c – f Granulocyte macrophage colony-stimulating factor-induced bone marrow-derived dendritic cells (gm-csf BMDCs) were infected with the A. fumigatus strains with the multiplicity of infection (MOI) = 5:1. Proinflammatory cytokines were quantified by <t>ELISA.</t> Data represent three biological replicates ( c – e ) or five biological replicates ( f ) with ± SEM. P- values were calculated by ANOVA with Tukey’s correction: *** P < 0.002; **** P < 0.0001. g , h Activation of dendritic cells measured by CD40 and CD80 markers by flow cytometry. Data represent three biological replicates with ±SEM. P -values were calculated by ANOVA: *** P < 0.0002; **** P < 0.0001 (for g ). *** P < 0.0004; **** P < 0.0001 (for h ). Source data are provided as a Source Data file.
Il 6 Duoset Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibody pairs mab206 15  (R&D Systems)
99
R&D Systems antibody pairs mab206 15
a , b A549 epithelial cells or macrophages were infected with the wild-type (WT), the nctA null mutant ( ∆nctA ) or the nctA reconstituted isolate ( nctA rec ) for 24 h. Cytotoxicity of each mutant was evaluated by measuring the release of lactate dehydrogenase (LDH) activity into the culture medium. The data represent five different infection challenges with triplicate LDH activity measurements. Data are shown in fold change of LDH activity relative to the wild-type-infected cells. The error bars mean the standard error of the mean (SEM), and P- values were calculated by Kruskal–Wallis test with Dunn’s correction: * P < 0.0180; ** P < 0.0056 (for a , A549 cells). ** P < 0.0032; *** P < 0.0007 (for b , macrophages). c – f Granulocyte macrophage colony-stimulating factor-induced bone marrow-derived dendritic cells (gm-csf BMDCs) were infected with the A. fumigatus strains with the multiplicity of infection (MOI) = 5:1. Proinflammatory cytokines were quantified by <t>ELISA.</t> Data represent three biological replicates ( c – e ) or five biological replicates ( f ) with ± SEM. P- values were calculated by ANOVA with Tukey’s correction: *** P < 0.002; **** P < 0.0001. g , h Activation of dendritic cells measured by CD40 and CD80 markers by flow cytometry. Data represent three biological replicates with ±SEM. P -values were calculated by ANOVA: *** P < 0.0002; **** P < 0.0001 (for g ). *** P < 0.0004; **** P < 0.0001 (for h ). Source data are provided as a Source Data file.
Antibody Pairs Mab206 15, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse il 6 elisa kit  (R&D Systems)
94
R&D Systems mouse il 6 elisa kit
a , b A549 epithelial cells or macrophages were infected with the wild-type (WT), the nctA null mutant ( ∆nctA ) or the nctA reconstituted isolate ( nctA rec ) for 24 h. Cytotoxicity of each mutant was evaluated by measuring the release of lactate dehydrogenase (LDH) activity into the culture medium. The data represent five different infection challenges with triplicate LDH activity measurements. Data are shown in fold change of LDH activity relative to the wild-type-infected cells. The error bars mean the standard error of the mean (SEM), and P- values were calculated by Kruskal–Wallis test with Dunn’s correction: * P < 0.0180; ** P < 0.0056 (for a , A549 cells). ** P < 0.0032; *** P < 0.0007 (for b , macrophages). c – f Granulocyte macrophage colony-stimulating factor-induced bone marrow-derived dendritic cells (gm-csf BMDCs) were infected with the A. fumigatus strains with the multiplicity of infection (MOI) = 5:1. Proinflammatory cytokines were quantified by <t>ELISA.</t> Data represent three biological replicates ( c – e ) or five biological replicates ( f ) with ± SEM. P- values were calculated by ANOVA with Tukey’s correction: *** P < 0.002; **** P < 0.0001. g , h Activation of dendritic cells measured by CD40 and CD80 markers by flow cytometry. Data represent three biological replicates with ±SEM. P -values were calculated by ANOVA: *** P < 0.0002; **** P < 0.0001 (for g ). *** P < 0.0004; **** P < 0.0001 (for h ). Source data are provided as a Source Data file.
Mouse Il 6 Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti rat il 6  (R&D Systems)
94
R&D Systems goat anti rat il 6
a , b A549 epithelial cells or macrophages were infected with the wild-type (WT), the nctA null mutant ( ∆nctA ) or the nctA reconstituted isolate ( nctA rec ) for 24 h. Cytotoxicity of each mutant was evaluated by measuring the release of lactate dehydrogenase (LDH) activity into the culture medium. The data represent five different infection challenges with triplicate LDH activity measurements. Data are shown in fold change of LDH activity relative to the wild-type-infected cells. The error bars mean the standard error of the mean (SEM), and P- values were calculated by Kruskal–Wallis test with Dunn’s correction: * P < 0.0180; ** P < 0.0056 (for a , A549 cells). ** P < 0.0032; *** P < 0.0007 (for b , macrophages). c – f Granulocyte macrophage colony-stimulating factor-induced bone marrow-derived dendritic cells (gm-csf BMDCs) were infected with the A. fumigatus strains with the multiplicity of infection (MOI) = 5:1. Proinflammatory cytokines were quantified by <t>ELISA.</t> Data represent three biological replicates ( c – e ) or five biological replicates ( f ) with ± SEM. P- values were calculated by ANOVA with Tukey’s correction: *** P < 0.002; **** P < 0.0001. g , h Activation of dendritic cells measured by CD40 and CD80 markers by flow cytometry. Data represent three biological replicates with ±SEM. P -values were calculated by ANOVA: *** P < 0.0002; **** P < 0.0001 (for g ). *** P < 0.0004; **** P < 0.0001 (for h ). Source data are provided as a Source Data file.
Goat Anti Rat Il 6, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse il 6 quantikine elisa kit  (R&D Systems)
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R&D Systems mouse il 6 quantikine elisa kit
a , b A549 epithelial cells or macrophages were infected with the wild-type (WT), the nctA null mutant ( ∆nctA ) or the nctA reconstituted isolate ( nctA rec ) for 24 h. Cytotoxicity of each mutant was evaluated by measuring the release of lactate dehydrogenase (LDH) activity into the culture medium. The data represent five different infection challenges with triplicate LDH activity measurements. Data are shown in fold change of LDH activity relative to the wild-type-infected cells. The error bars mean the standard error of the mean (SEM), and P- values were calculated by Kruskal–Wallis test with Dunn’s correction: * P < 0.0180; ** P < 0.0056 (for a , A549 cells). ** P < 0.0032; *** P < 0.0007 (for b , macrophages). c – f Granulocyte macrophage colony-stimulating factor-induced bone marrow-derived dendritic cells (gm-csf BMDCs) were infected with the A. fumigatus strains with the multiplicity of infection (MOI) = 5:1. Proinflammatory cytokines were quantified by <t>ELISA.</t> Data represent three biological replicates ( c – e ) or five biological replicates ( f ) with ± SEM. P- values were calculated by ANOVA with Tukey’s correction: *** P < 0.002; **** P < 0.0001. g , h Activation of dendritic cells measured by CD40 and CD80 markers by flow cytometry. Data represent three biological replicates with ±SEM. P -values were calculated by ANOVA: *** P < 0.0002; **** P < 0.0001 (for g ). *** P < 0.0004; **** P < 0.0001 (for h ). Source data are provided as a Source Data file.
Mouse Il 6 Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotinylated rat anti mouse il 6  (R&D Systems)
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R&D Systems biotinylated rat anti mouse il 6
a , b A549 epithelial cells or macrophages were infected with the wild-type (WT), the nctA null mutant ( ∆nctA ) or the nctA reconstituted isolate ( nctA rec ) for 24 h. Cytotoxicity of each mutant was evaluated by measuring the release of lactate dehydrogenase (LDH) activity into the culture medium. The data represent five different infection challenges with triplicate LDH activity measurements. Data are shown in fold change of LDH activity relative to the wild-type-infected cells. The error bars mean the standard error of the mean (SEM), and P- values were calculated by Kruskal–Wallis test with Dunn’s correction: * P < 0.0180; ** P < 0.0056 (for a , A549 cells). ** P < 0.0032; *** P < 0.0007 (for b , macrophages). c – f Granulocyte macrophage colony-stimulating factor-induced bone marrow-derived dendritic cells (gm-csf BMDCs) were infected with the A. fumigatus strains with the multiplicity of infection (MOI) = 5:1. Proinflammatory cytokines were quantified by <t>ELISA.</t> Data represent three biological replicates ( c – e ) or five biological replicates ( f ) with ± SEM. P- values were calculated by ANOVA with Tukey’s correction: *** P < 0.002; **** P < 0.0001. g , h Activation of dendritic cells measured by CD40 and CD80 markers by flow cytometry. Data represent three biological replicates with ±SEM. P -values were calculated by ANOVA: *** P < 0.0002; **** P < 0.0001 (for g ). *** P < 0.0004; **** P < 0.0001 (for h ). Source data are provided as a Source Data file.
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cold air 827 il 6  (R&D Systems)
97
R&D Systems cold air 827 il 6
a , b A549 epithelial cells or macrophages were infected with the wild-type (WT), the nctA null mutant ( ∆nctA ) or the nctA reconstituted isolate ( nctA rec ) for 24 h. Cytotoxicity of each mutant was evaluated by measuring the release of lactate dehydrogenase (LDH) activity into the culture medium. The data represent five different infection challenges with triplicate LDH activity measurements. Data are shown in fold change of LDH activity relative to the wild-type-infected cells. The error bars mean the standard error of the mean (SEM), and P- values were calculated by Kruskal–Wallis test with Dunn’s correction: * P < 0.0180; ** P < 0.0056 (for a , A549 cells). ** P < 0.0032; *** P < 0.0007 (for b , macrophages). c – f Granulocyte macrophage colony-stimulating factor-induced bone marrow-derived dendritic cells (gm-csf BMDCs) were infected with the A. fumigatus strains with the multiplicity of infection (MOI) = 5:1. Proinflammatory cytokines were quantified by <t>ELISA.</t> Data represent three biological replicates ( c – e ) or five biological replicates ( f ) with ± SEM. P- values were calculated by ANOVA with Tukey’s correction: *** P < 0.002; **** P < 0.0001. g , h Activation of dendritic cells measured by CD40 and CD80 markers by flow cytometry. Data represent three biological replicates with ±SEM. P -values were calculated by ANOVA: *** P < 0.0002; **** P < 0.0001 (for g ). *** P < 0.0004; **** P < 0.0001 (for h ). Source data are provided as a Source Data file.
Cold Air 827 Il 6, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse il 6 antibody  (R&D Systems)
95
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a , b A549 epithelial cells or macrophages were infected with the wild-type (WT), the nctA null mutant ( ∆nctA ) or the nctA reconstituted isolate ( nctA rec ) for 24 h. Cytotoxicity of each mutant was evaluated by measuring the release of lactate dehydrogenase (LDH) activity into the culture medium. The data represent five different infection challenges with triplicate LDH activity measurements. Data are shown in fold change of LDH activity relative to the wild-type-infected cells. The error bars mean the standard error of the mean (SEM), and P- values were calculated by Kruskal–Wallis test with Dunn’s correction: * P < 0.0180; ** P < 0.0056 (for a , A549 cells). ** P < 0.0032; *** P < 0.0007 (for b , macrophages). c – f Granulocyte macrophage colony-stimulating factor-induced bone marrow-derived dendritic cells (gm-csf BMDCs) were infected with the A. fumigatus strains with the multiplicity of infection (MOI) = 5:1. Proinflammatory cytokines were quantified by <t>ELISA.</t> Data represent three biological replicates ( c – e ) or five biological replicates ( f ) with ± SEM. P- values were calculated by ANOVA with Tukey’s correction: *** P < 0.002; **** P < 0.0001. g , h Activation of dendritic cells measured by CD40 and CD80 markers by flow cytometry. Data represent three biological replicates with ±SEM. P -values were calculated by ANOVA: *** P < 0.0002; **** P < 0.0001 (for g ). *** P < 0.0004; **** P < 0.0001 (for h ). Source data are provided as a Source Data file.
Mouse Il 6 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat il 6 elisa quantikine kit  (R&D Systems)
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R&D Systems rat il 6 elisa quantikine kit
a , b A549 epithelial cells or macrophages were infected with the wild-type (WT), the nctA null mutant ( ∆nctA ) or the nctA reconstituted isolate ( nctA rec ) for 24 h. Cytotoxicity of each mutant was evaluated by measuring the release of lactate dehydrogenase (LDH) activity into the culture medium. The data represent five different infection challenges with triplicate LDH activity measurements. Data are shown in fold change of LDH activity relative to the wild-type-infected cells. The error bars mean the standard error of the mean (SEM), and P- values were calculated by Kruskal–Wallis test with Dunn’s correction: * P < 0.0180; ** P < 0.0056 (for a , A549 cells). ** P < 0.0032; *** P < 0.0007 (for b , macrophages). c – f Granulocyte macrophage colony-stimulating factor-induced bone marrow-derived dendritic cells (gm-csf BMDCs) were infected with the A. fumigatus strains with the multiplicity of infection (MOI) = 5:1. Proinflammatory cytokines were quantified by <t>ELISA.</t> Data represent three biological replicates ( c – e ) or five biological replicates ( f ) with ± SEM. P- values were calculated by ANOVA with Tukey’s correction: *** P < 0.002; **** P < 0.0001. g , h Activation of dendritic cells measured by CD40 and CD80 markers by flow cytometry. Data represent three biological replicates with ±SEM. P -values were calculated by ANOVA: *** P < 0.0002; **** P < 0.0001 (for g ). *** P < 0.0004; **** P < 0.0001 (for h ). Source data are provided as a Source Data file.
Rat Il 6 Elisa Quantikine Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a , b A549 epithelial cells or macrophages were infected with the wild-type (WT), the nctA null mutant ( ∆nctA ) or the nctA reconstituted isolate ( nctA rec ) for 24 h. Cytotoxicity of each mutant was evaluated by measuring the release of lactate dehydrogenase (LDH) activity into the culture medium. The data represent five different infection challenges with triplicate LDH activity measurements. Data are shown in fold change of LDH activity relative to the wild-type-infected cells. The error bars mean the standard error of the mean (SEM), and P- values were calculated by Kruskal–Wallis test with Dunn’s correction: * P < 0.0180; ** P < 0.0056 (for a , A549 cells). ** P < 0.0032; *** P < 0.0007 (for b , macrophages). c – f Granulocyte macrophage colony-stimulating factor-induced bone marrow-derived dendritic cells (gm-csf BMDCs) were infected with the A. fumigatus strains with the multiplicity of infection (MOI) = 5:1. Proinflammatory cytokines were quantified by ELISA. Data represent three biological replicates ( c – e ) or five biological replicates ( f ) with ± SEM. P- values were calculated by ANOVA with Tukey’s correction: *** P < 0.002; **** P < 0.0001. g , h Activation of dendritic cells measured by CD40 and CD80 markers by flow cytometry. Data represent three biological replicates with ±SEM. P -values were calculated by ANOVA: *** P < 0.0002; **** P < 0.0001 (for g ). *** P < 0.0004; **** P < 0.0001 (for h ). Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: The negative cofactor 2 complex is a key regulator of drug resistance in Aspergillus fumigatus

doi: 10.1038/s41467-019-14191-1

Figure Lengend Snippet: a , b A549 epithelial cells or macrophages were infected with the wild-type (WT), the nctA null mutant ( ∆nctA ) or the nctA reconstituted isolate ( nctA rec ) for 24 h. Cytotoxicity of each mutant was evaluated by measuring the release of lactate dehydrogenase (LDH) activity into the culture medium. The data represent five different infection challenges with triplicate LDH activity measurements. Data are shown in fold change of LDH activity relative to the wild-type-infected cells. The error bars mean the standard error of the mean (SEM), and P- values were calculated by Kruskal–Wallis test with Dunn’s correction: * P < 0.0180; ** P < 0.0056 (for a , A549 cells). ** P < 0.0032; *** P < 0.0007 (for b , macrophages). c – f Granulocyte macrophage colony-stimulating factor-induced bone marrow-derived dendritic cells (gm-csf BMDCs) were infected with the A. fumigatus strains with the multiplicity of infection (MOI) = 5:1. Proinflammatory cytokines were quantified by ELISA. Data represent three biological replicates ( c – e ) or five biological replicates ( f ) with ± SEM. P- values were calculated by ANOVA with Tukey’s correction: *** P < 0.002; **** P < 0.0001. g , h Activation of dendritic cells measured by CD40 and CD80 markers by flow cytometry. Data represent three biological replicates with ±SEM. P -values were calculated by ANOVA: *** P < 0.0002; **** P < 0.0001 (for g ). *** P < 0.0004; **** P < 0.0001 (for h ). Source data are provided as a Source Data file.

Article Snippet: The concentration of IL-8 and IL-6 were determined in A549 epithelial cells co-cultured with A. fumigatus strains by using the Human IL-8/CXCL8 and IL-6 DuoSet ELISA according to the manufacturer’s instructions (R&D systems).

Techniques: Infection, Mutagenesis, Activity Assay, Derivative Assay, Enzyme-linked Immunosorbent Assay, Activation Assay, Flow Cytometry