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Image Search Results
Journal: Journal of Translational Medicine
Article Title: Phenylpropanoid-enriched broccoli seedling extract can reduce inflammatory markers and pain behavior
doi: 10.1186/s12967-023-04777-1
Figure Lengend Snippet: OE reduces inflammation in rabbit disc cells in vitro, increases collagen gene expression in vivo. A – C Rabbit disc cells were pretreated with different concentrations of OE and then stimulated with lipopolysaccharide (LPS) for 16 h. Gene expression of Interleukin-8 (IL-8) and nitric oxide synthase 2 (NOS2) was analyzed. Statistically significant differences between OE-treated and VC (saline) from LPS (P < 0.05) are indicated (*). Conditioned media was collected to quantify interleukin-8 (IL-8) protein. (Lower panel D) Rabbits (n = 8) underwent surgical disc injury and intradiscal treatment with OE or VC. After 3 weeks, intervertebral disc tissues were isolated for RNA analysis of phenotypic disc and inflammatory genes: collagen type I (COL1), collagen type II (COL2), chemokine C–C motif ligand (CCL)2, CCL5 and IL-8 ( D ). Cell culture experiment data were analyzed with One way ANOVA with multiple comparisons and included in the significant difference (*) on graphs. In vivo, rabbit disc gene expression was analyzed with multiple unpaired t-tests. Error bars represent SEM
Article Snippet: Taqman gene expression assays were used for analysis or normalization of the following genes: rabbit collagen type I (COL1), collagen type II (COL2), IL-8, hypoxanthine phosphoribosyltransferase 1 (HPRT1) and 18 S rRNA (Oc03396113_m1, Oc03396134_m1,
Techniques: In Vitro, Gene Expression, In Vivo, Saline, Isolation, Cell Culture
Journal: Frontiers in Veterinary Science
Article Title: A Live-Attenuated Equine Influenza Vaccine Stimulates Innate Immunity in Equine Respiratory Epithelial Cell Cultures That Could Provide Protection From Equine Herpesvirus 1
doi: 10.3389/fvets.2021.674850
Figure Lengend Snippet: Primer and probe source list.
Article Snippet: IL-8 , TaqMan ® gene expression assay no:
Techniques: Gene Expression
Journal: Frontiers in Veterinary Science
Article Title: A Live-Attenuated Equine Influenza Vaccine Stimulates Innate Immunity in Equine Respiratory Epithelial Cell Cultures That Could Provide Protection From Equine Herpesvirus 1
doi: 10.3389/fvets.2021.674850
Figure Lengend Snippet: Cell viability and chemokine mRNA expression following Flu Avert treatment in ERECs. (A) Microscopic analysis of EREC cultures. Mean grades from cells ± SEM of three horses following Flu Avert treatment at different MOIs. Grading of cell viability from 0 to 3 is described in . (B) Cell viability analysis. Mean percent positive cells ± SEM of three horses at each MOI as determined with propidium iodide staining. Black circle is MOI = 0. Black square is MOI = 0.1. Black upright triangle is MOI = 1. Black upside-down triangle is MOI = 5. (C) IL-8 mRNA expression. (D) CXCL9 mRNAs expression. (E) CCL2 mRNA expression. Values are mean log fold change (–ddCq) ± SEM. Black square represents untreated (media) treated ERECs. Black circle represents Flu Avert-treated ERECs. * p ≤ 0.05 and ** p < 0.01, respectively, between the media and Flu Avert treatment groups.
Article Snippet: IL-8 , TaqMan ® gene expression assay no:
Techniques: Expressing, Staining
Journal: Frontiers in Veterinary Science
Article Title: A Live-Attenuated Equine Influenza Vaccine Stimulates Innate Immunity in Equine Respiratory Epithelial Cell Cultures That Could Provide Protection From Equine Herpesvirus 1
doi: 10.3389/fvets.2021.674850
Figure Lengend Snippet: Effect of Flu Avert treatment on chemokine mRNA expression in ERECs collected from five horses 24 h following EHV-1 inoculation. (A) IL-8 mRNA expression in ERECs that were treated with Flu Avert on day 5 prior to EHV-1 inoculation. (B) IL-8 mRNA expression in ERECs that were treated with Flu Avert on day 7 prior to EHV-1 inoculation. (C) CXCL10 mRNA expression in ERECs that were treated with Flu Avert on day 5 prior to EHV-1 inoculation. (D) CXCL10 mRNA expression in ERECs that were treated with Flu Avert on day 7 prior to EHV-1 inoculation. The mean log fold change (–ddCq) is represented by a bar (* p < 0.05).
Article Snippet: IL-8 , TaqMan ® gene expression assay no:
Techniques: Expressing
Journal: Frontiers in Veterinary Science
Article Title: A Live-Attenuated Equine Influenza Vaccine Stimulates Innate Immunity in Equine Respiratory Epithelial Cell Cultures That Could Provide Protection From Equine Herpesvirus 1
doi: 10.3389/fvets.2021.674850
Figure Lengend Snippet: Effect of Flu Avert treatment on chemokine mRNA expression in ERECs collected from five horses 48 h following EHV-1 inoculation. (A) IL-8 mRNA expression in ERECs that were treated with Flu Avert on day 1 prior to EHV-1 inoculation. (B) IL-8 mRNA expression in ERECs that were treated with Flu Avert on day 2 prior to EHV-1 inoculation. (C) IL-8 mRNA expression in ERECs that were treated with Flu Avert on day 5 prior to EHV-1 inoculation. (D) IL-8 mRNA expression in ERECs that were treated with Flu Avert on day 7 prior to EHV-1 inoculation. (E) CCL2 mRNA expression in ERECs that were treated with Flu Avert on day 1 prior to EHV-1 inoculation. (F) CCL2 mRNA expression in ERECs that were treated with Flu Avert on day 2 prior to EHV-1 inoculation. (G) CCL2 mRNA expression in ERECs that were treated with Flu Avert on day 5 prior to EHV-1 inoculation. (H) CCL2 mRNA expression in ERECs that were treated with Flu Avert on day 7 prior to EHV-1 inoculation. The mean log fold change (–ddCq) is represented by a bar (** p < 0.01 and * p < 0.05).
Article Snippet: IL-8 , TaqMan ® gene expression assay no:
Techniques: Expressing
Journal: Irish Veterinary Journal
Article Title: The mechanistic role of natural antimicrobials in preventing Staphylococcus aureus invasion of MAC-T cells using an in vitro mastitis model
doi: 10.1186/s13620-024-00265-0
Figure Lengend Snippet: The impact of As on S. aureus ClfB expression and MAC-T cells inflammatory response. A describes the experimental design (1-exposure of S. aureus to MAC-T cells; 2-adhesion/infection of MAC-T cells with S. aureus to; 3-As inclusion during infection and inhibition of ClfB; 4-MAC-T cells pro-inflammatory response). B shows the impact of As on IL6, IL1β, IL8 and TNFα expression in infected MAC-T cells. C indicates the effect of As on ClfB expression in S. aureus without the presence of MAC-T cells; the expression ClfB in S-aureus co-cocultured with MAC-T cells and As is presented in panel D , E indicates the expression of ClfB in S. aureus MAC-T cells attached or internalised after 6 h of exposure to 0.1, 0.2, 0.5 and 1% As. All experiments were performed in triplicate and the results are represented as means ± SD. Student’s t test was performed to assess significance with the P values being indicated on graphs. The ClfB gene expression is presented as fold change over the infected and As unexposed control. Panel A was created using Biorender
Article Snippet: The primers used. cDNA (1 μl) was added to duplicate wells with 7 μl of DNase-free water, 10 μl of Taqman Fast Advanced master mix (
Techniques: Expressing, Infection, Inhibition, Gene Expression, Control