Journal: Respiratory Research

Article Title: IL-5 signaling in asthmatic derived fibroblasts exacerbates airway remodeling through ECM dysregulation and apoptosis resistance

doi: 10.1186/s12931-025-03371-x

Figure Lengend Snippet: IL-5 inducing the expression of IL-5Ra in lung derived fibroblasts. A mRNA levels of IL5RA in both normal and asthmatic derived lung fibroblasts. 18s was used as a housekeeping gene for ΔCt normalization, and fold changes upon IL-5 treatment compared to control was calculated as 2. −ΔΔct . Data shown are the mean ± SD of four separate experiment; two-tailed paired student’s t-Test statistical analysis was done * p < 0.05, ** p < 0.01, *** p < 0.001 . B Cultured normal and asthmatic fibroblasts, captured at 40X magnification, display immunofluorescence staining for IL-5Rα (green), vimentin (red), and DAPI (blue) in both cell types, along with mouse and rabbit IgG isotype controls. C Evaluation of the mean fluorescence intensity (MFI) of IL-5Rα expression in lung fibroblasts, quantified following 24 h of IL-5 stimulation. Statistical significance was determined using two-tailed paired Student's t-test based on data from three independent experiments, * p < 0.05, ** p < 0.01, and *** p < 0.001

Article Snippet: Subsequently, the cells were incubated overnight at 4 °C with primary antibodies against IL-5Rα (Cat: MAB253, R&D Systems) Vimentin (Cat: 5741, Cell Signalling Technology), Rabbit mAb IgG XP® Isotype Control (Cat: 3900, CST), Mouse IgG isotype control (Cat: MAB002, R&D systems) at a 1:100 dilution.

Techniques: Expressing, Derivative Assay, Control, Two Tailed Test, Cell Culture, Immunofluorescence, Staining, Fluorescence