il 23 Search Results


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Influenza and Gram‐positive bacteria induce IFN‐α production in pDC and IL‐12 <t>p40</t> production in mDC, respectively. Cord blood‐derived mDC (A and C) and pDC (B and D) were cultured together with allogenic CD4 + T cells with different strains of bacteria (A and B) or virus (C and D). Supernatants were collected after 48 h and analysed for IL‐12 p40 (A and C) or IFN‐α (B and D) content. Data represent the individual and the mean of secreted cytokines in picogram per millilitre from 3 to 6 MLRs using one‐way anova followed by Dunnet’s multiple comparison test.
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Influenza and Gram‐positive bacteria induce IFN‐α production in pDC and IL‐12 <t>p40</t> production in mDC, respectively. Cord blood‐derived mDC (A and C) and pDC (B and D) were cultured together with allogenic CD4 + T cells with different strains of bacteria (A and B) or virus (C and D). Supernatants were collected after 48 h and analysed for IL‐12 p40 (A and C) or IFN‐α (B and D) content. Data represent the individual and the mean of secreted cytokines in picogram per millilitre from 3 to 6 MLRs using one‐way anova followed by Dunnet’s multiple comparison test.
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Influenza and Gram‐positive bacteria induce IFN‐α production in pDC and IL‐12 <t>p40</t> production in mDC, respectively. Cord blood‐derived mDC (A and C) and pDC (B and D) were cultured together with allogenic CD4 + T cells with different strains of bacteria (A and B) or virus (C and D). Supernatants were collected after 48 h and analysed for IL‐12 p40 (A and C) or IFN‐α (B and D) content. Data represent the individual and the mean of secreted cytokines in picogram per millilitre from 3 to 6 MLRs using one‐way anova followed by Dunnet’s multiple comparison test.
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Influenza and Gram‐positive bacteria induce IFN‐α production in pDC and IL‐12 p40 production in mDC, respectively. Cord blood‐derived mDC (A and C) and pDC (B and D) were cultured together with allogenic CD4 + T cells with different strains of bacteria (A and B) or virus (C and D). Supernatants were collected after 48 h and analysed for IL‐12 p40 (A and C) or IFN‐α (B and D) content. Data represent the individual and the mean of secreted cytokines in picogram per millilitre from 3 to 6 MLRs using one‐way anova followed by Dunnet’s multiple comparison test.

Journal: Scandinavian Journal of Immunology

Article Title: Enveloped Virus but not Bacteria Block IL‐13 Responses in Human Cord Blood T Cells In Vitro

doi: 10.1111/j.1365-3083.2012.02676.x

Figure Lengend Snippet: Influenza and Gram‐positive bacteria induce IFN‐α production in pDC and IL‐12 p40 production in mDC, respectively. Cord blood‐derived mDC (A and C) and pDC (B and D) were cultured together with allogenic CD4 + T cells with different strains of bacteria (A and B) or virus (C and D). Supernatants were collected after 48 h and analysed for IL‐12 p40 (A and C) or IFN‐α (B and D) content. Data represent the individual and the mean of secreted cytokines in picogram per millilitre from 3 to 6 MLRs using one‐way anova followed by Dunnet’s multiple comparison test.

Article Snippet: ELISA: IL‐12 p40 levels were determined using an IL‐12 p40 DuoSet ELISA according to manufacturer’s instructions (R&D, Minneapolis, MN, USA).

Techniques: Bacteria, Derivative Assay, Cell Culture, Virus, Comparison