Journal: Cell reports

Article Title: Evaluation of Single-Cell Cytokine Secretion and Cell-Cell Interactions with a Hierarchical Loading Microwell Chip

doi: 10.1016/j.celrep.2020.107574

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: To prepare functionalized beads to detect IFN-γ or IL-2, a biotinylated antibody against human IFN-γ (Thermo Fisher, M701B) or a biotinylated antibody against human IL-2 (R&D Systems, BAF202) was conjugated to streptavidin polystyrene beads (mean size: 18.4 μm, Spherotech, SVP-200–4) or streptavidin magnetic beads (mean size: 21.7 μm, Spherotech, SVM-200–4) by shaking beads in 50 μg/mL IFN-γ antibody or IL-2 antibody solution at 4°C overnight in PBS, respectively.

Techniques: Recombinant, Labeling, Software