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Image Search Results
Journal: bioRxiv
Article Title: A genome-wide CRISPR screen in human prostate cancer cells reveals drivers of macrophage-mediated cell killing and positions AR as a tumor-intrinsic immunomodulator
doi: 10.1101/2023.06.06.543873
Figure Lengend Snippet: A. Schematic overview of IKK signaling. Canonical NF-κB is regulated by the IKK complex, which consists of the IKKα, β, and γ subunits. Phosphorylation of IκBα leads to its degradation, allowing the p65-p50 dimer to accumulate in the nucleus and regulate transcription of target genes. B. Relative mRNA levels of IKBKB, IKBKG and CHUK (normalized to TBP ) in IKBKB-KO , IKBKG-KO and CHUK-KO LNCaP cells (n=3, mean values are depicted and error bars represent the standard deviation). P-value was calculated using a two-way ANOVA test. C. Protein levels of IKBKB, IKBKG and CHUK protein levels in IKBKB-KO , IKBKG-KO and CHUK-KO LNCaP cells. Actin and HSP90 are used as loading control. Cas9 was used as a control for efficient lentiCRISPRv2 transfection. D. Crystal violet assay of NT control and IKBKB-KO , IKBKG-KO and CHUK-KO cells cultured as a monoculture (up) and co-culture (down) with macrophages. Cells were cultured for 3 days. Crystal violet staining was quantified using Fiji software.
Article Snippet: After transfer, membranes were stained with Ponceau S and subsequently blocked in 4% BSA (A8022, Sigma/Merck) in 1x PBS-Tween (137 mM NACl, 10 mM Na 2 HP04, 1.5 mM KH 2 PO 4 , 2.6 mM KCl, 0.1% Tween-20) for 1h and incubated with primary antibodies against IKKα (2682, Cell signaling, 1:1000),
Techniques: Phospho-proteomics, Standard Deviation, Control, Transfection, Crystal Violet Assay, Cell Culture, Co-Culture Assay, Staining, Software