Journal: Scientific Reports

Article Title: Targeted therapy of pyrrolo[2,3- d ]pyrimidine antifolates in a syngeneic mouse model of high grade serous ovarian cancer and the impact on the tumor microenvironment

doi: 10.1038/s41598-022-14788-5

Figure Lengend Snippet: Expression of GARFTase and AICARFTase transcripts in primary EOC patient samples and BR-5 and BR-Luc cells. Transcript levels for GARFTase ( A ) and AICARFTase ( B ) were measured using cDNAs from primary specimens including normal ovary (n = 8) and HGSOC (n = 39) (OriGene) and results were compared to those for EOC cell lines including IGROV1, SKOV3, A2780 and A2780 E-80. Transcript levels were normalized to β-actin transcripts. Statistical analyses were performed between normal samples/tissues and tumor samples/tissues using the Wilcoxon rank-sum test. ( C , D ) Transcript levels of cytosolic C1 metabolic targets (GARFTase and AICARFTase) were determined in the BR-5 and BR-Luc syngeneic mouse models of HGSOC by real-time RT-PCR. Transcripts were normalized to β-actin transcripts and results are shown relative to levels in mouse liver (assigned a value of 1). Results are presented as mean values ± standard errors from at least three experiments. The p values are as follows: **** p < 0.0001. See Supplementary Table for patient characteristics and pathology.

Article Snippet: Transcript levels for GARFTase ( A ) and AICARFTase ( B ) were measured using cDNAs from primary specimens including normal ovary (n = 8) and HGSOC (n = 39) (OriGene) and results were compared to those for EOC cell lines including IGROV1, SKOV3, A2780 and A2780 E-80.

Techniques: Expressing, Quantitative RT-PCR

Journal: Scientific Reports

Article Title: Targeted therapy of pyrrolo[2,3- d ]pyrimidine antifolates in a syngeneic mouse model of high grade serous ovarian cancer and the impact on the tumor microenvironment

doi: 10.1038/s41598-022-14788-5

Figure Lengend Snippet: IC 50 values (in nM) for anti-proliferative activities of novel compounds on HGSOC mouse models BR-Luc and BR-5 compared to cisplatin.

Article Snippet: Transcript levels for GARFTase ( A ) and AICARFTase ( B ) were measured using cDNAs from primary specimens including normal ovary (n = 8) and HGSOC (n = 39) (OriGene) and results were compared to those for EOC cell lines including IGROV1, SKOV3, A2780 and A2780 E-80.

Techniques:

Journal: Oncotarget

Article Title: Antiproliferative effects of two gold(I)-N-heterocyclic carbene complexes in A2780 human ovarian cancer cells: a comparative proteomic study

doi: 10.18632/oncotarget.25556

Figure Lengend Snippet: Antiproliferative effects of Au(NHC), Au(NHC) 2 and cisplatin on cell growth of SKOV3 and IGROV1 ovarian cancer cells after 72 h drug exposure

Article Snippet: IGROV1 and SKOV3 were a gift of Prof. Joseph R. Bertino (New Brunswick, NJ, USA).

Techniques:

Journal: Oncotarget

Article Title: Antiproliferative effects of two gold(I)-N-heterocyclic carbene complexes in A2780 human ovarian cancer cells: a comparative proteomic study

doi: 10.18632/oncotarget.25556

Figure Lengend Snippet: Percentage of apoptotic cells shown by flow cytometry analysis of annexin V/propidium iodide-stained IGROV1 (A) and SKOV3 (B) cells treated for 72 h with Au(NHC) and Au(NHC) 2 -72 h-exposure IC 50 -dose. Flow cytometric images are representative of three independent experiments. Histograms report the mean values ±SD. The statistical analysis was carried out using one-way ANOVA test followed by Tuckey's multiple comparisons test using Graphpad Prism v 6.0 ( ** p<0.01).

Article Snippet: IGROV1 and SKOV3 were a gift of Prof. Joseph R. Bertino (New Brunswick, NJ, USA).

Techniques: Flow Cytometry, Staining

Journal: Oncotarget

Article Title: Antiproliferative effects of two gold(I)-N-heterocyclic carbene complexes in A2780 human ovarian cancer cells: a comparative proteomic study

doi: 10.18632/oncotarget.25556

Figure Lengend Snippet: Western blot analysis of the glycolytic enzymes GAPDH, ALDOA, TPI1 (A) and of the mitochondrial enzyme CS (B) in IGROV1 and in SKOV3 cells upon exposure to Au(NHC) and Au(NHC) 2 . Representative Immunoblots are shown together with the corresponding Coomassie-stained PVDF membranes. Histogram reports normalized mean relative-integrated-density ± SD values of the GAPDH, ALDOA, TPI1 and CS bands. The statistical analysis was carried out using one-way ANOVA test followed by Tuckey's multiple comparisons test using Graphpad Prism v 6.0 ( * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001).

Article Snippet: IGROV1 and SKOV3 were a gift of Prof. Joseph R. Bertino (New Brunswick, NJ, USA).

Techniques: Western Blot, Staining

Journal: Oncotarget

Article Title: Antiproliferative effects of two gold(I)-N-heterocyclic carbene complexes in A2780 human ovarian cancer cells: a comparative proteomic study

doi: 10.18632/oncotarget.25556

Figure Lengend Snippet: IGROV1 and SKOV3 cells were treated with Au(NHC) and Au(NHC) 2 for 48 h with their 72 h-exposure IC 50 -dose (A) Lactate amount was detected in one millilitre of medium supernatant using a commercial L-lactic acid Assay Kit (Megazyme); (B) Oxygen consumption was measured using a Clark-type O 2 electrode from Hansatech. (For details, see Materials and Methods). Histograms report the mean values ±SD of at least three independent experiments. The statistical analysis was carried out using one-way ANOVA test followed by Tuckey's multiple comparisons test using Graphpad Prism v 6.0 ( * p<0.05, ** p<0.01, *** p<0.001).

Article Snippet: IGROV1 and SKOV3 were a gift of Prof. Joseph R. Bertino (New Brunswick, NJ, USA).

Techniques: Acid Assay

Journal: Oncotarget

Article Title: Antiproliferative effects of two gold(I)-N-heterocyclic carbene complexes in A2780 human ovarian cancer cells: a comparative proteomic study

doi: 10.18632/oncotarget.25556

Figure Lengend Snippet: IGROV1 and SKOV3 cells were treated with Au(NHC) and Au(NHC) 2 -72 h-IC 50 -dose. TrxR enzyme inhibition assay was performed after 24 h of treatment, using a commercial thioredoxin reductase assay kit (Sigma-Aldrich), in (A) IGROV1 and (B) SKOV3 cells. For details, see Materials and Methods. Histograms report the mean values ±SD. The statistical analysis was carried out using one-way ANOVA test followed by Tuckey's multiple comparisons test using Graphpad Prism v 6.0 ( *** p<0.001, **** p<0.0001).

Article Snippet: IGROV1 and SKOV3 were a gift of Prof. Joseph R. Bertino (New Brunswick, NJ, USA).

Techniques: Enzyme Inhibition Assay, Reductase Assay

Journal: British journal of pharmacology

Article Title: BBIT20 inhibits homologous DNA repair with disruption of the BRCA1–BARD1 interaction in breast and ovarian cancer

doi: 10.1111/bph.15506

Figure Lengend Snippet: Antiproliferative effect of BBIT20, cisplatin and olaparib, in a panel of human breast cancer, ovarian cancer and non-malignant cells

Article Snippet: A total of 2.5 × 10 6 IGROV-1 cells (expressing heterozygous mutBRCA1) were implanted subcutaneously (in PBS/Matrigel 1:1; Corning, Enzifarma, Porto, Portugal) in the right flank of female mice with 6 to 8 weeks.

Techniques: