Journal: eLife

Article Title: SNAP25 disease mutations change the energy landscape for synaptic exocytosis due to aberrant SNARE interactions

doi: 10.7554/eLife.88619

Figure Lengend Snippet:

Article Snippet: Electrophysiological data were analyzed in IGOR Pro (v6.21 and v6.37, WaveMetrics) using a custom-written script. mEPSCs were analyzed with MiniAnalysis (v6.0.7, Synaptosoft).

Techniques: Transfection, Construct, Recombinant, In Vitro, Expressing, Sequencing, Bicinchoninic Acid Protein Assay, Mutagenesis, Western Blot, Buffer Exchange, Software

Journal: bioRxiv

Article Title: CellWell: A micropatterned biphasic nanocomposite platform for culturing chondrocytes

doi: 10.1101/790030

Figure Lengend Snippet: Murine femoral cap hip explants (A) and primary human articular chondrocytes (B) and (C) were plated on fibronectin-coated coverglass using standard 2D cell culture techniques, fixed with 4% paraformaldehyde, stained with ActinRed 555 Ready probes® Reagent (ThermoFisher) and imaged in super-resolution using 3D structured illumination microscopy (3D-SIM) on a GE DeltaScan™ OMX SR microscope. Maximum intensity projections of volumetric image stacks are shown. Note in the side view of (A) that the turbidity of the tissue prevented imaging of all but the surface-most structures in the explant images, a common problem in imaging of both natural tissues and 3D cell cultures. Scale bars are 2 μm and apply to both top and side projections.

Article Snippet: The viscoelastic properties of CellWells and articular cartilage were analyzed using an Asylum Research MFP 3D Atomic Force Microscope (AFM), with Igor Pro v6.37.

Techniques: Cell Culture, Staining, Microscopy, Imaging