Journal: Insects

Article Title: Effect of Feed Containing Hermetia illucens Larvae Immunized by Lactobacillus plantarum Injection on the Growth and Immunity of Rainbow Trout ( Oncorhynchus mykiss )

doi: 10.3390/insects12090801

Figure Lengend Snippet: Measuring immunity-enhancing degree in rainbow trout fed with imHIL-containing feed. Aspartate aminotransferase (AST) ( A ), lysozyme activity ( B ), and immunoglobulin M (IgM) ( C ) were quantitatively analyzed. Initial AST level of rainbow trout blood serum was 67.57 ± 16.72 U L −1 . Values are means ± standard deviations of triplicates. HIL0, 0% HIL; HI25, 25% normal HIL inclusion as fishmeal weight basis; HIL50, 50% normal HIL inclusion as fishmeal weight basis; ImHIL25, 25% immunized HIL inclusion as fishmeal weight basis; ImHIL50, 50% immunized HIL inclusion as fishmeal weight basis. Vertical error bars denote standard error of mean, and alphabetic characters represent significant differences ( p < 0.05) among each group at week 3 and week 6 as determined by Duncan’s multiple range test, respectively ( n = 9, 3 tanks, 3 fish per tank).

Article Snippet: IgM in the blood serum of O. mykiss was measured using an enzyme-linked immunosorbent assay (ELISA) kit (Fish immunoglobulin M (IgM) ELISA kit, Cusabio, Houston, TX, USA) and analyzed according to the manufacturer’s instructions.

Techniques: Activity Assay

Journal: BMC veterinary research

Article Title: Synergistic fermentation of Cordyceps militaris and herbal substrates boosts grower pig antioxidant and immune function.

doi: 10.1186/s12917-024-04338-8

Figure Lengend Snippet: Fig. 5 The effect of CMSSF supplementation levels on swine serum Immunoglobulins (A) IgA, (B) IgM, (C) IgG concentrations via ELISA and (D) CSFV antibody level (S/P Ratio). Control general feed, L:1 g CMSSF/kg feed, M:5 g CMSSF/kg feed, H:20 g CMSSF/kg feed, PC: Tiamulin 100 ppm + Oxytetracycline 220 ppm. The p-values of < 0.05 were regarded as statistically significant, expressed as * p < 0.05; ** p < 0.01; **** p < 0.0001

Article Snippet: The concentrations of IgA, IgG, and IgM were analyzed using the pig ELISA kits (#E100-102, #E100-104, and #E101-117, Bethyl Laboratories, Inc.).

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: eLife

Article Title: Expression of modified FcγRI enables myeloid cells to elicit robust tumor-specific cytotoxicity

doi: 10.7554/eLife.91999

Figure Lengend Snippet:

Article Snippet: Commercial assay, kit , Mouse IgM ELISA Kit , Bethyl , Catalog # E99-101 , .

Techniques: Transfection, Construct, Recombinant, Plasmid Preparation, Enzyme-linked Immunosorbent Assay

Journal: Journal of Gastroenterology

Article Title: Anti-integrin αvβ6 autoantibodies in patients with primary sclerosing cholangitis

doi: 10.1007/s00535-023-02006-6

Figure Lengend Snippet: IgG subclasses and isotypes of the anti-integrin αvβ6 antibodies in sera from patients with PSC. a IgG subclasses of the anti-integrin αvβ6 antibodies were quantified by an ELISA. The cutoff OD, defined as the mean plus three standard deviations of the control sera, is indicated by a dashed line. Thirty (81.1%), 8 (21.6%), 5 (13.5%), and 4 (10.8%) of the 37 patients with PSC had IgG1, IgG2, IgG3, and IgG4 antibodies, respectively. Conversely, 1, 1, 0, and 1 of the 16 controls had IgG1, IgG2, IgG3, and IgG4 antibodies, respectively. b Isotypes of anti-integrin αvβ6 antibodies were quantified by ELISA. Twenty-one (56.8%), 0 (0%), and 4 (10.8%) of the 37 patients with PSC had IgA, IgM, and IgE antibodies against integrin αvβ6, respectively. Conversely, none of the 16 controls had IgA, IgM, or IgE antibodies. IgG immunoglobulin G, PSC primary sclerosing cholangitis, ELISA enzyme-linked immunosorbent assay, OD optical density

Article Snippet: The purity of the IgG fraction was confirmed by testing for IgA, IgM, IgE, and protein contaminants using a Human IgA ELISA kit (E88-102, Bethyl Laboratories), a Human IgM ELISA kit (E88-100, Bethyl Laboratories), a Human IgE ELISA kit (E88-108, Bethyl Laboratories), and sodium dodecyl sulfate–polyacrylamide gel electrophoresis with Coomassie Brilliant Blue staining, respectively.

Techniques: Enzyme-linked Immunosorbent Assay, Control