Journal: The Journal of biological chemistry

Article Title: Rapid redistribution of CD20 to a low density detergent-insoluble membrane compartment.

doi: 10.1074/jbc.273.1.344

Figure Lengend Snippet: FIG. 1. FACScan profiles of CD20 mAb binding to Raji cells. Indirect immunofluorescence was performed as described under “Ex- perimental Procedures.” Solid profile represents the binding of the fluorescein isothiocyanate-labeled secondary antibody. Open profiles show binding of the primary antibodies 1F5, 2H7, and B1 as indicated.

Article Snippet: Bands were visualized using Kodak X-OMAT film (Eastman Kodak Co.) Immunofluorescence—Cells (2 3 105) were suspended and incubated in 100 ml of RPMI 1640 medium, 10% fetal bovine serum for 15 min at 37 °C with 2H7, 1F5, B1, or isotype-matched control mAb, washed once, and resuspended for 15 min with 100 ml of 1/100 dilution of goat anti-mouse IgG conjugated to fluorescein isothiocyanate (Southern Biotechnology Associates, Inc.).

Techniques: Binding Assay, Immunofluorescence, Labeling

Journal: The Journal of biological chemistry

Article Title: Rapid redistribution of CD20 to a low density detergent-insoluble membrane compartment.

doi: 10.1074/jbc.273.1.344

Figure Lengend Snippet: FIG. 5. 2H7-induced CD20 redistribution to the Triton-insolu- ble fraction is rapid and does not involve internalization. A, CD20 immunoblot. Cells were treated with 2H7 mAb for the times indicated and lysed immediately with 2 3 lysis buffer. Each lane con- tains Triton-insoluble material from 2 3 105 cells. B, indirect immuno- fluorescence. Solid profile represents the binding of the fluorescein isothiocyanate-labeled secondary antibody. Open profiles, cells were incubated for 15 min at 37 °C with 2H7 and then washed. Fluorescein isothiocyanate-conjugated secondary antibody was either added im- mediately (solid line) or after a further 45-min incubation at 37 °C (dashed line).

Article Snippet: Bands were visualized using Kodak X-OMAT film (Eastman Kodak Co.) Immunofluorescence—Cells (2 3 105) were suspended and incubated in 100 ml of RPMI 1640 medium, 10% fetal bovine serum for 15 min at 37 °C with 2H7, 1F5, B1, or isotype-matched control mAb, washed once, and resuspended for 15 min with 100 ml of 1/100 dilution of goat anti-mouse IgG conjugated to fluorescein isothiocyanate (Southern Biotechnology Associates, Inc.).

Techniques: Western Blot, Lysis, Fluorescence, Binding Assay, Labeling, Incubation

Journal: Vaccine

Article Title: Liposomes containing monophosphoryl lipid A and QS-21 serve as an effective adjuvant for soluble circumsporozoite protein malaria vaccine FMP013.

doi: 10.1016/j.vaccine.2017.05.070

Figure Lengend Snippet: Fig. 2. Subclasses and Avidity. (A) Mean anti-FL CSP IgG1 (left) and IgG2c (right) titers (n = 8) from first challenge study at 2 weeks post-third dose. (B) Mean avidity index values from FL (left) and NANP (right) avidity ELISA. P-values are for Tukey’s test for multiple comparisons of log transformed titers. Open bars are group means, whiskers represent SEM.

Article Snippet: HRP-linked goat anti-mouse IgG1 and anti-mouse IgG2c (SouthernBiotech, Birmingham, AL) were used for detection of subclass-specific serum IgG.

Techniques: Enzyme-linked Immunosorbent Assay, Transformation Assay