Journal: Pharmaceutics

Article Title: Multicompartmental Lipopolyplex as Vehicle for Antigens and Genes Delivery in Vaccine Formulations

doi: 10.3390/pharmaceutics13020281

Figure Lengend Snippet: Serological responses to immunization with GD3 antigen after the first, second, or third dose of vaccine. Animals were immunized with liposomes bearing the GD3 antigen (SM: CH: GD3 or SM: CH: DP: GD3) or with lipopolyplexes bearing GD3 and the pMOK plasmid bearing GM-CSF cytokine gene. Titers of anti-GD3 immunoglobulins were determined by ELISA. The titers correspond with the maximal dilution of mice serum that yielded an optical density above 0.100. These determinations were performed in triplicate and only results with dispersion lower than 5% were accepted.

Article Snippet: As secondary antibody for total immunoglobulins, goat antisera to total IgG (Biocheck, Scarborough, USA) was used at 1/10,000 dilution.

Techniques: Liposomes, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Dispersion, Control

Journal: Pharmaceutics

Article Title: Multicompartmental Lipopolyplex as Vehicle for Antigens and Genes Delivery in Vaccine Formulations

doi: 10.3390/pharmaceutics13020281

Figure Lengend Snippet: Characterization of the specific isotypes of anti-GD3 immunoglobulins obtained after the third vaccination dose. Immunoglobulin titration was determined by ELISA. The titers correspond with the maximal dilution of mice serum that yielded an optical density above 0.100. These determinations were performed in triplicate and only results with dispersion lower than 5% were accepted.

Article Snippet: As secondary antibody for total immunoglobulins, goat antisera to total IgG (Biocheck, Scarborough, USA) was used at 1/10,000 dilution.

Techniques: Titration, Enzyme-linked Immunosorbent Assay, Dispersion, Control, Liposomes