Journal: Frontiers in Immunology

Article Title: SFRP4 + IGFBP5 hi NKT cells induced neural-like cell differentiation to contribute to adenomyosis pain

doi: 10.3389/fimmu.2022.945504

Figure Lengend Snippet: SFRP4 + IGFBP5 hi NKT cells induced neural-like cell differentiation and pain-related factor expression. (A) Immunofluorescence revealed a considerable number of nerve fibers in the AM pain group, as well as elevated IGFBP5 production by SFRP4 + NKT cells. PGP5.5 stands for nerve fibers. NKG7 and SFRP4 stand for NKT cells. (B) Based on pseudotime analysis, a gene heat map displaying the expression of nerve development and pain-related components was under development. Validation of RT-PCR for associated mRNA expression in AM tissues. (C) The IGFBP5 protein expression level was correlated positively with the VAS score, while the NEFM mRNA expression level was correlated positively with the duration of discomfort. The data were distributed normally.

Article Snippet: Tissue sections were incubated overnight at 4°C with the following anti-IGFBP5(1:200, CST, Danvers, Massachusetts, USA), anti-PGP5.5 (1:200, abcam, Danvers, Massachusetts, USA), anti-CD8 APC (1:250, thermo, Waltham, MA, USA), anti-NKG7 (1:200, affinity, Suzhou, China), and anti-SFRP4 (1:200, abcam, Waltham, MA, USA).

Techniques: Cell Differentiation, Expressing, Immunofluorescence, Biomarker Discovery, Reverse Transcription Polymerase Chain Reaction

Journal: Breast cancer research and treatment

Article Title: A novel role of IGFBP5 in the migration, invasion and spheroids formation induced by IGF-I and insulin in MCF-7 breast cancer cells.

doi: 10.1007/s10549-024-07397-5

Figure Lengend Snippet: Fig. 1 IGFBP5 regulates IGF- IR expression and migration in MCF-7 breast cancer cells. a–c Lysates from wild type MCF-7 cells and sh-IGFBP5 MCF-7 cells were analyzed by WB with anti-IGFBP5 Ab, anti-IGF-IR Ab, anti-IR Ab and anti-actin Ab. d Confluent cultures of wild type MCF-7 cells and sh-IGFBP5 MCF-7 cells were scratched and untreated and treated with 50 ng/ml IGF- I, 100 and 340 nM insulin. Graphs are the mean ± S.D. and indicate the fold of protein expression (IGFBP5, IGF-IR, IR) or migration above control value. *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: IGFBP5 antibody (Ab), insulin receptor β Ab, FAK Ab and lentiviral shRNA vectors against IGFBP5 were from Santa Cruz Biotechnology (St. Cruz, CA).

Techniques: Expressing, Migration, Control

Journal: Breast cancer research and treatment

Article Title: A novel role of IGFBP5 in the migration, invasion and spheroids formation induced by IGF-I and insulin in MCF-7 breast cancer cells.

doi: 10.1007/s10549-024-07397-5

Figure Lengend Snippet: Fig. 2 IGFBP5 inhibits MMP-9 secretion induced by IGF-I and insulin. a Wild type MCF-7 cells and sh-IGFBP5 MCF-7 cells were treated with 50 ng/ml IGF-I, 100 and 340 nM insulin for 48 h and then conditioned media were obtained. MMP-2 and MMP-9 secre- tion was analyzed by gelatin-substrate gels. b Invasion assays were performed using the Boyden chamber method with wild type MCF-7 cells and sh-IGFBP5 MCF-7 cells treated with 50 ng/ml IGF-I, 100 nM insulin for 48 h. Graphs are the mean ± S.D. and indicate the fold of MMP-9 secretion or invasion above control value. *P < 0.05, ***P < 0.001

Article Snippet: IGFBP5 antibody (Ab), insulin receptor β Ab, FAK Ab and lentiviral shRNA vectors against IGFBP5 were from Santa Cruz Biotechnology (St. Cruz, CA).

Techniques: Control

Journal: Breast cancer research and treatment

Article Title: A novel role of IGFBP5 in the migration, invasion and spheroids formation induced by IGF-I and insulin in MCF-7 breast cancer cells.

doi: 10.1007/s10549-024-07397-5

Figure Lengend Snippet: Fig. 3 IGFBP5 inhibits FAK phosphorylation at Ser-910 induced by insulin. a Wild type MCF-7 cells and sh-IGFBP5 MCF-7 cells were treated with 50 ng/ml IGF-I, 100 and 340 nM insulin for 10 min and lysed. b Wild type MCF-7 cells and sh-IGFBP5 MCF-7 cells were untreated and treated with 340 nM insulin for 5, 10, 20, 30, 45 and 60 min and lysed. Cell lysates were analyzed by WB with anti-p-FAK Ser 910 Ab, anti-FAK Ab and anti-actin Ab. One control of untreated cells and incubated for 60 min was included. c Wild type MCF-7 cells and sh-IGFBP5 MCF-7 cells were treated with 50 ng/ ml IGF-I, 100 and 340 nM insulin for 20 min and lysed. Cell lysates were analyzed by WB with anti-p-FAK Ab. anti- FAK Ab and anti-actin Ab. One control of FBS was included. Graphs are the mean ± S.D. and indicate the fold of FAK phosphorylation at Ser 910 and Tyr 397 above control value. **P < 0.01, ***P < 0.001

Article Snippet: IGFBP5 antibody (Ab), insulin receptor β Ab, FAK Ab and lentiviral shRNA vectors against IGFBP5 were from Santa Cruz Biotechnology (St. Cruz, CA).

Techniques: Phospho-proteomics, Control, Incubation

Journal: Breast cancer research and treatment

Article Title: A novel role of IGFBP5 in the migration, invasion and spheroids formation induced by IGF-I and insulin in MCF-7 breast cancer cells.

doi: 10.1007/s10549-024-07397-5

Figure Lengend Snippet: Fig. 4 IGFBP5 inhibits IGF-IR expression. a and b Wild type MCF-7 cells and sh-IGFBP5 MCF-7 cells were treated with 50 ng/ml IGF-I, 100 and 340 nM insulin for 2 h and lysed. Cell lysates were analyzed by WB with anti-IGF-IR Ab, anti-IR Ab and anti-actin Ab. Graphs are the mean ± S.D. and indicate the fold of IGF-IR or IR above con- trol value.**P < 0.01, ***P < 0.001

Article Snippet: IGFBP5 antibody (Ab), insulin receptor β Ab, FAK Ab and lentiviral shRNA vectors against IGFBP5 were from Santa Cruz Biotechnology (St. Cruz, CA).

Techniques: Expressing

Journal: Breast cancer research and treatment

Article Title: A novel role of IGFBP5 in the migration, invasion and spheroids formation induced by IGF-I and insulin in MCF-7 breast cancer cells.

doi: 10.1007/s10549-024-07397-5

Figure Lengend Snippet: Fig. 5 Role of IGFBP5 in the spheroids formation induced by IGF-I and insulin. a Representa- tive images of spheroids using wild type MCF-7 cells and sh- IGFBP5 MCF-7 cells untreated and treated with 50 ng/ml IGF- I, 340 nM insulin and 10% FBS. b Number of spheroids using wild type MCF-7 cells and sh- IGFBP5 MCF-7 cells untreated and treated with 50 ng/ml IGF-I, 340 nM insulin and 10% FBS. c Size of spheroids using wild type MCF-7 cells and sh- IGFBP5 MCF-7 cells untreated and treated with 50 ng/ml IGF- I, 340 nM insulin and 10% FBS. Graphs are the mean ± S.D. and indicate the spheroids number or size of spheroids above wild type value. **P < 0.01

Article Snippet: IGFBP5 antibody (Ab), insulin receptor β Ab, FAK Ab and lentiviral shRNA vectors against IGFBP5 were from Santa Cruz Biotechnology (St. Cruz, CA).

Techniques:

Journal: Frontiers in Endocrinology

Article Title: Short-Term Diet Induced Changes in the Central and Circulating IGF Systems Are Sex Specific

doi: 10.3389/fendo.2020.00513

Figure Lengend Snippet: List of Taqman probes used for RT-PCR.

Article Snippet: Insulin-like growth factor-binding protein 5 ( Igfbp5 ) , Rn00563116_m1.

Techniques: Clinical Proteomics

Journal: Frontiers in Endocrinology

Article Title: Short-Term Diet Induced Changes in the Central and Circulating IGF Systems Are Sex Specific

doi: 10.3389/fendo.2020.00513

Figure Lengend Snippet: Serum levels of free insulin-like growth factor (IGF)-1 (A) , total IGF-1 (B) , IGF 2 (C) , IGF binding protien (IGFBP) 2 (D) , IGFBP3 (E) , IGFBP5 (F) , and pregnancy-associated plasma protein (PAPP-A)2 (G) in rats on a high fat diet (HFD), low fat diet (LFD) or a chow diet for 1 week. ** p < 0.01; *** p < 0.001. a, overall effect of sex. n = 6.

Article Snippet: Insulin-like growth factor-binding protein 5 ( Igfbp5 ) , Rn00563116_m1.

Techniques: Binding Assay, Clinical Proteomics

Journal: Frontiers in Endocrinology

Article Title: Short-Term Diet Induced Changes in the Central and Circulating IGF Systems Are Sex Specific

doi: 10.3389/fendo.2020.00513

Figure Lengend Snippet: Relative gene expression of members of the IGF system in the hypothalamus.

Article Snippet: Insulin-like growth factor-binding protein 5 ( Igfbp5 ) , Rn00563116_m1.

Techniques: Gene Expression