Journal: Scientific Reports

Article Title: The metabolic effects of resumption of a high fat diet after weight loss are sex dependent in mice

doi: 10.1038/s41598-023-40514-w

Figure Lengend Snippet: Circulating levels of free insulin-like growth factor (IGF) 1 ( A ), total IGF1 ( B ), IGF2 ( C ), IGF binding protein (IGFBP) 2 ( D ) and leptin ( E ) in mice fed chow or a high-fat diet (HFD) for 4 months (HHHH), chow diet for 3 months and an additional month on a HFD (CCCH), or HFD for 2 months followed by chow for 1 month and then HFD for the last month (HHCH). ***p < 0.001. a: effect of the sex, b: effect of the diet. NS non-significant. n = 9.

Article Snippet: Plasma levels of free IGF1 (Ref.: AL-136, AnshLabs, Webster, TX, USA), total IGF1 (Ref.: E25; Mediagnost, Reutlingen, Germany), IGF2 (Ref.: MG200; R&D Systems, Minneapolis, Minnesota, USA), IGFBP2 (Ref.: RAB0234; Millipore, Burlington, MA, USA), insulin (Ref.: EZRMI-13K; Millipore) and leptin (Ref.: EZML-82K; Millipore) were assayed according to manufacturer’s instructions, and absorbance was read using a TECAN Infinite M200.

Techniques: Binding Assay

Journal: Scientific Reports

Article Title: The metabolic effects of resumption of a high fat diet after weight loss are sex dependent in mice

doi: 10.1038/s41598-023-40514-w

Figure Lengend Snippet: Relative mRNA levels of insulin-like growth factor (IGF) 1 ( A ), IGF2 ( B ), IGF binding protein (IGFBP)2 ( C ), neuropeptide Y (NPY; E ), Agouti-related protein (AgRP; F ), and proopiomelanocortin (POMC; G ) in the hypothalamus, as well as the correlation of hypothalamic IGF2 and IGFBP2 mRNA levels ( D ), in mice fed chow or a high-fat diet (HFD) for 4 months (HHHH), a chow diet for 3 months and an additional month on a HFD (CCCH), or HFD for 2 months followed by chow for 1 month and then HFD for the last month (HHCH). *p < 0.05, ***p < 0.001. a: effect of the sex, b: effect of the diet. n = 6.

Article Snippet: Plasma levels of free IGF1 (Ref.: AL-136, AnshLabs, Webster, TX, USA), total IGF1 (Ref.: E25; Mediagnost, Reutlingen, Germany), IGF2 (Ref.: MG200; R&D Systems, Minneapolis, Minnesota, USA), IGFBP2 (Ref.: RAB0234; Millipore, Burlington, MA, USA), insulin (Ref.: EZRMI-13K; Millipore) and leptin (Ref.: EZML-82K; Millipore) were assayed according to manufacturer’s instructions, and absorbance was read using a TECAN Infinite M200.

Techniques: Binding Assay

Journal: Scientific Reports

Article Title: The metabolic effects of resumption of a high fat diet after weight loss are sex dependent in mice

doi: 10.1038/s41598-023-40514-w

Figure Lengend Snippet: Cell number ( A ) and the relative IGF1 ( B ), IGF2 receptor (IGF2R, C ) and IGFBP2 ( D ) mRNA levels in hypothalamic astrocyte cultures from male and female rats after 24 h of IGF2 treatment at 10, 25, 100 or 200 ng/ml. **p < 0.01. a: effect of the sex; #, general effect of IGF2; δ, effect of the dose of IGF2. n = 3.

Article Snippet: Plasma levels of free IGF1 (Ref.: AL-136, AnshLabs, Webster, TX, USA), total IGF1 (Ref.: E25; Mediagnost, Reutlingen, Germany), IGF2 (Ref.: MG200; R&D Systems, Minneapolis, Minnesota, USA), IGFBP2 (Ref.: RAB0234; Millipore, Burlington, MA, USA), insulin (Ref.: EZRMI-13K; Millipore) and leptin (Ref.: EZML-82K; Millipore) were assayed according to manufacturer’s instructions, and absorbance was read using a TECAN Infinite M200.

Techniques:

Journal: Scientific Reports

Article Title: The metabolic effects of resumption of a high fat diet after weight loss are sex dependent in mice

doi: 10.1038/s41598-023-40514-w

Figure Lengend Snippet: List of TaqMan probes used for qPCR.

Article Snippet: Plasma levels of free IGF1 (Ref.: AL-136, AnshLabs, Webster, TX, USA), total IGF1 (Ref.: E25; Mediagnost, Reutlingen, Germany), IGF2 (Ref.: MG200; R&D Systems, Minneapolis, Minnesota, USA), IGFBP2 (Ref.: RAB0234; Millipore, Burlington, MA, USA), insulin (Ref.: EZRMI-13K; Millipore) and leptin (Ref.: EZML-82K; Millipore) were assayed according to manufacturer’s instructions, and absorbance was read using a TECAN Infinite M200.

Techniques:

Journal: Cell Reports Medicine

Article Title: Unacylated-Ghrelin Impairs Hippocampal Neurogenesis and Memory in Mice and Is Altered in Parkinson’s Dementia in Humans

doi: 10.1016/j.xcrm.2020.100120

Figure Lengend Snippet:

Article Snippet: For IGF-1 and GH, platelet-rich plasma samples were analyzed using Human IGF-1 DuoSet ELISA (cat. No. DY291, R&D Systems), and Human GH DuoSet ELISA (Cat. No. DY1067, R&D Systems), using half-volume Nunclon Microwell 96-well plates.

Techniques: Clinical Proteomics, Recombinant, Saline, RNAscope, Bicinchoninic Acid Protein Assay, Enzyme-linked Immunosorbent Assay, Software, Microscopy