Journal: Frontiers in Microbiology

Article Title: Presence of Segmented Filamentous Bacteria in Human Children and Its Potential Role in the Modulation of Human Gut Immunity

doi: 10.3389/fmicb.2018.01403

Figure Lengend Snippet: (A) Overall relationship between the level of sIgA in luminal fluids from the SFB-positive and SFB-negative patients. (B) Relationship between the level of sIgA and the ages of children. A total number of 47 samples (23 SFB-positive and 24 SFB-negative) were used. 500 μl of luminal fluids from each patient were used for the measurement of sIgA as described in the Section “Materials and Methods.” The supernatants were assayed for total secretory IgA using a human IgA ELISA Detection Kit as described in the section “Materials and Methods.” The sIgA was expressed as μg/ml of fecal materials. P -values were calculated using Student’s t -test.

Article Snippet: Samples were incubated at room temperature for 2 h. Total sIgA was quantified by ELISA using a human secretory IgA ELISA detection Kit (Elabscience, Wuhan, China).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: iScience

Article Title: Altered B cell immunoglobulin signature exhibits potential diagnostic values in human colorectal cancer

doi: 10.1016/j.isci.2023.106140

Figure Lengend Snippet: Biased IgG subclass distribution in CRC (A) FACS analysis was performed to determine the phenotypic characteristics of B cells in normal mucosa and B cells in tumor tissue. Representative markers expressed (N = 7). (B) Statistical analysis of the phenotypic characteristics and statistical significance were determined using the Mann-Whitney U test, N = 5, results represent mean ± SEM of five donors (N = 7), ∗p < 0.05, ∗∗p < 0.01. (C and D) Confocal microscopy analysis and statistical analysis of IgA+ and IgG+ cells (red) in CD79a+ B cells (green). (E) The conditioned medium was collected from isolated CD45 + CD19 + B cells after 24 h, and IgM, IgA, and IgG level in the conditioned medium was determined by ELISA. Results represent mean ± SEM of four independent experiments (N = 5). ∗∗p < 0.01; ∗∗∗p < 0.001 (Paired Student’s t test).

Article Snippet: Human IgA ELISA Kit , Bethyl Laboratories , Cat#E88-102.

Techniques: MANN-WHITNEY, Confocal Microscopy, Isolation, Enzyme-linked Immunosorbent Assay

Journal: iScience

Article Title: Altered B cell immunoglobulin signature exhibits potential diagnostic values in human colorectal cancer

doi: 10.1016/j.isci.2023.106140

Figure Lengend Snippet:

Article Snippet: Human IgA ELISA Kit , Bethyl Laboratories , Cat#E88-102.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Software

Journal: Nature communications

Article Title: Generation of colonic IgA-secreting cells in the caecal patch.

doi: 10.1038/ncomms4704

Figure Lengend Snippet: Figure 2 | Decreased IgA-secreting cells in large intestinal lamina propria of appendectomized germ-free mice after conventionalization. (a,b) Lamina propria lymphocytes were isolated from appendectomized or sham-operated germ-free(GF) mice at 4 weeks after conventionalization, and stained for surface IgA/B220 or intracellular IL-17/IFN-g/IL-10 in CD4 þ population. (a) Total numbers of IgA þ B220 cells as well as IL-17-, IFN-g- and IL-10-positive CD4 þ cells in the large (upper) and the small intestine (lower) are shown. Each symbol represents an individual mouse, and horizontal bars indicate the mean. (b) Representative dot plots for cells stained with anti-IgA and B220 of appendectomized and sham-operated mice. Numbers within plots indicate percentages of cells in respective areas. (c) Distribution of IgA þ cells in the small and large intestines. Frozen sections were stained with anti-IgA mAb (green) and DAPI (blue). Scale bars, 100 mm. (d) Faeces and sera were collected from appendectomized (n ¼ 12) or sham-operated mice (n ¼ 12) at 4 weeks after conventionalization and GF mice (n ¼ 12), and IgA levels of faeces and sera were determined by ELISA. Each symbol represents an individual mouse, and horizontal bars indicate the mean. Statistical analyses were performed with the Student’s t-test. *Po0.05. NS, not significant.

Article Snippet: IgA levels in the faeces, sera and culture supernatants were determined by a mouse IgA ELISA quantitation kit (Bethyl Laboratories).

Techniques: Isolation, Staining, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Medicine

Article Title: Predicting cardiometabolic disease in medical students using FibroScan and 30-year Framingham risk scores

doi: 10.3389/fmed.2024.1431935

Figure Lengend Snippet: Comparison of participants with and without elevated liver stiffness.

Article Snippet: To measure human immunoglobulin A2 and human PIGR levels, we used a commercially available enzyme-linked immunosorbent assay kit from Elabscience Biotechnology Inc., United States.

Techniques: Comparison, Control, Clinical Proteomics