ifnγ-af647 Search Results


90
Becton Dickinson anti-ifn-γ af647
Evaluation of T cell associated <t>IFN-γ</t> and TNF-α responses from Alfort-187 and C-strain infected pigs in the tonsil. Porcine tonsil mononuclear cells were stimulated by the addition of CSFV Alfort-187. Following overnight culture, cells were stained and the cytokine expression associated with each of the T cell populations was evaluated by flow cytometry. Histograms show the mean IFN-γ and TNF-α secretion associated with each T cell population. Values and the medians were plotted; boxes represent the 25/75% precentile. Statistical significances were assessed by a two-way ANOVA with Bonferroni correction again.
Anti Ifn γ Af647, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-ifn-γ af647/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
anti-ifn-γ af647 - by Bioz Stars, 2026-02
90/100 stars
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93
Bio-Rad anti ifn γ af647

Anti Ifn γ Af647, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti ifn γ af647/product/Bio-Rad
Average 93 stars, based on 1 article reviews
anti ifn γ af647 - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier

90
Bio-Rad ifnγ-af647

Ifnγ Af647, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ifnγ-af647/product/Bio-Rad
Average 90 stars, based on 1 article reviews
ifnγ-af647 - by Bioz Stars, 2026-02
90/100 stars
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90
Becton Dickinson anti-mouse ifnγ-alexafluor-647

Anti Mouse Ifnγ Alexafluor 647, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-mouse ifnγ-alexafluor-647/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
anti-mouse ifnγ-alexafluor-647 - by Bioz Stars, 2026-02
90/100 stars
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Image Search Results


Evaluation of T cell associated IFN-γ and TNF-α responses from Alfort-187 and C-strain infected pigs in the tonsil. Porcine tonsil mononuclear cells were stimulated by the addition of CSFV Alfort-187. Following overnight culture, cells were stained and the cytokine expression associated with each of the T cell populations was evaluated by flow cytometry. Histograms show the mean IFN-γ and TNF-α secretion associated with each T cell population. Values and the medians were plotted; boxes represent the 25/75% precentile. Statistical significances were assessed by a two-way ANOVA with Bonferroni correction again.

Journal: International Journal of Molecular Sciences

Article Title: Activation of Dendritic Cells in Tonsils Is Associated with CD8 T Cell Responses following Vaccination with Live Attenuated Classical Swine Fever Virus

doi: 10.3390/ijms22168795

Figure Lengend Snippet: Evaluation of T cell associated IFN-γ and TNF-α responses from Alfort-187 and C-strain infected pigs in the tonsil. Porcine tonsil mononuclear cells were stimulated by the addition of CSFV Alfort-187. Following overnight culture, cells were stained and the cytokine expression associated with each of the T cell populations was evaluated by flow cytometry. Histograms show the mean IFN-γ and TNF-α secretion associated with each T cell population. Values and the medians were plotted; boxes represent the 25/75% precentile. Statistical significances were assessed by a two-way ANOVA with Bonferroni correction again.

Article Snippet: After washing twice with BD Perm/Wash (BD Pharmingen) the cells were incubated for a further 30 min at 4 °C with anti-IFN-γ AF647 (clone P2G10, BD Biosciences) and anti-TNF BV421 (clone MAb 11, Biolegend) diluted in BD Perm/Wash.

Techniques: Infection, Staining, Expressing, Flow Cytometry

Correlation between CSFV-specific IFN-γ + CD8 + T cells, viral loads and activation status of cDC1 and cDC2 in tonsils. ( A ) Correlation between CSFV-specific IFN-γ + CD8 + T cells and MHC-II expression levels of cDC1 cells ( n = 24). ( B ) Correlation between CSFV-specific IFN-γ + CD8 + T cells and MHC-II expression levels of cDC2 in pigs ( n = 24). ( C ) Correlation between CSFV-specific IFN-γ + CD8 + T cells and viral loads at 36 and 90 h post-infection, when viral loads were detected in the tonsil ( n = 16). Statistics were evaluated by a Spearman correlation test (Spearman r and p -values shown in graph).

Journal: International Journal of Molecular Sciences

Article Title: Activation of Dendritic Cells in Tonsils Is Associated with CD8 T Cell Responses following Vaccination with Live Attenuated Classical Swine Fever Virus

doi: 10.3390/ijms22168795

Figure Lengend Snippet: Correlation between CSFV-specific IFN-γ + CD8 + T cells, viral loads and activation status of cDC1 and cDC2 in tonsils. ( A ) Correlation between CSFV-specific IFN-γ + CD8 + T cells and MHC-II expression levels of cDC1 cells ( n = 24). ( B ) Correlation between CSFV-specific IFN-γ + CD8 + T cells and MHC-II expression levels of cDC2 in pigs ( n = 24). ( C ) Correlation between CSFV-specific IFN-γ + CD8 + T cells and viral loads at 36 and 90 h post-infection, when viral loads were detected in the tonsil ( n = 16). Statistics were evaluated by a Spearman correlation test (Spearman r and p -values shown in graph).

Article Snippet: After washing twice with BD Perm/Wash (BD Pharmingen) the cells were incubated for a further 30 min at 4 °C with anti-IFN-γ AF647 (clone P2G10, BD Biosciences) and anti-TNF BV421 (clone MAb 11, Biolegend) diluted in BD Perm/Wash.

Techniques: Activation Assay, Expressing, Infection

Journal: STAR Protocols

Article Title: Multiparameter flow cytometry assay to analyze the pulmonary T cell profiles in the ovine model of respiratory syncytial virus infection

doi: 10.1016/j.xpro.2022.101688

Figure Lengend Snippet:

Article Snippet: Anti-IFN-γ-AF647 (Clone CC302; mouse IgG1; 1:160 dilution) , Bio-Rad , Cat# MCA1783A647.

Techniques: Virus, Recombinant, Sterility, Staining, Cell Culture, Hood, Flow Cytometry, Software