Journal: Frontiers in Aging Neuroscience

Article Title: Royal Jelly Alleviates Cognitive Deficits and β-Amyloid Accumulation in APP/PS1 Mouse Model Via Activation of the cAMP/PKA/CREB/BDNF Pathway and Inhibition of Neuronal Apoptosis

doi: 10.3389/fnagi.2018.00428

Figure Lengend Snippet: RJ treatment reduced the brain Aβ burden of APP/PS1 mice. (A,B) The levels of SDS-soluble and -insoluble (formic acid soluble) Aβ were measured using enzyme-linked immunosorbent assays (ELISAs). (C) The Aβ plaques in the cortex and hippocampus were estimated after immunohistochemical (IHC) staining with the Aβ antibody (B-4), the number of plaques per view ( D,E ; cortex, 20× magnification; hippocampus, 40× magnification) and proportions of the positive area (F,G) were calculated, three sections per animal. Scale bar = 200 μm. The arrows point out the representative senile plaques (SPs). Data are presented as the mean ± SEM. * P < 0.05, ** P < 0.01, n.s. non-significant. (H–L) Immunoblot analysis of β-secretase (BACE1), insulin-degrading enzyme (IDE), neprilysin (NEP) and lipoprotein receptor-related protein-1 (LRP-1) in the brain homogenates. n = 6–10 mice per group. Data are presented as the mean ± SEM. * P < 0.05, ** P < 0.01, n.s. non-significant.

Article Snippet: Briefly, sections were blocked with 3% BSA for 30 min, then incubated with primary antibodies overnight at 4°C, including BACE1 (1:100, Santa Cruz Biotechnology, USA), IDE (1:100, Santa Cruz Biotechnology, USA), β-amyloid (B-4, 1:100, Santa Cruz Biotechnology, USA).

Techniques: Immunohistochemical staining, Immunohistochemistry, Western Blot

Journal: Stem Cell Research & Therapy

Article Title: Rapid and high-efficiency generation of mature functional hepatocyte-like cells from adipose-derived stem cells by a three-step protocol

doi: 10.1186/s13287-015-0181-3

Figure Lengend Snippet: Hepatic lineage differentiation condition

Article Snippet: First, the cells were treated for 1 day of endodermal induction with RPMI-1640 (Gibco) supplemented with 100 nM IDE1 (Tocris, Chengdu, Sichuan, P.R.

Techniques:

Journal: Diabetologia

Article Title: Presence of immunogenic alternatively spliced insulin gene product in human pancreatic delta cells

doi: 10.1007/s00125-023-05882-y

Figure Lengend Snippet: IDE is not expressed in delta cells and cleaves INS-splice. ( a ) Pancreatic IDE protein expression was determined by immunohistochemistry of human pancreas sections by staining for proinsulin (green), IDE (white) and somatostatin (red). IDE was expressed ubiquitously in the exocrine and endocrine pancreas except in delta cells. Scale bar, 10 μm. Nuclei were visualised by Hoechst staining (blue). ( b ) Co-localisation of IDE with insulin and IDE with somatostatin was quantified using MCC (QuPath). Thirty-six islets from six pancreas donors were analysed. Data are shown as mean ± SD and statistical analysis was performed using a paired two-tailed Student’s t test (*** p <0.001). ( c ) Coomassie staining of INS-splice after IDE cleavage assay. Absence or presence of IDE is indicated by − or +, respectively. Full-length recombinant INS-splice is 14 kDa. INS, insulin; M, protein marker; SST, somatostatin

Article Snippet: Recombinant alternatively spliced insulin product (INS-splice; 0.25 μg/μl) was incubated with recombinant human IDE (0.05 μg/μl, 2496-ZN; R&D Systems, USA) in cleavage buffer (50 mmol/l Tris, 1 mol/l NaCl, pH 7.5) at 37°C for 48 h, following the manufacturer’s recommendations.

Techniques: Expressing, Immunohistochemistry, Staining, Two Tailed Test, Cleavage Assay, Recombinant, Marker