Journal: iScience

Article Title: Peripheral immunophenotyping of AITD subjects reveals alterations in immune cells in pediatric vs adult-onset AITD

doi: 10.1016/j.isci.2021.103626

Figure Lengend Snippet: Unsupervised clustering and dimension reduction analysis of myeloid and NK cells reveals similar frequencies of subpopulations in AITD patients and controls (A) Non-T/non-B cells which encompass myeloid and natural killer (NK) cells were manually gated as CD45 + CD3-CD19-and then analyzed using the unsupervised clustering and dimension reduction programs PhenoGraph and X-shift to generate distinct clusters based on cell surface marker expression (CD15, CD1c, CD11b, HLA-DR, CD56, CD11c, CD14, and CD16). A UMAP plot was created using total non-B/non-T cells from concatenated adult AITD, adult healthy control, pediatric AITD, and pediatric healthy control samples with a minimum of 17,000 cell events. Seven unique cell subpopulations were identified. UMAP plots of each surface marker are displayed to demonstrate varying expression levels among the myeloid and NK cell subpopulations. (B) Heatmap of cell surface marker expression for identification of the 5 myeloid and 2 NK cell subsets identified using PhenoGraph/X-Shift. cDC1: conventional type 1 dendritic cell; cDC2: conventional type 2 dendritic cell. (C) Frequencies of myeloid and NK cell subsets in adult AITD (AA), adult healthy control (AH), pediatric AITD (PA), and pediatric healthy control (PH) subjects demonstrate normal frequencies in AITD patients compared to controls. Data are represented as mean ± SEM. Samples were processed as depicted in Figure S1 . Statistical significance determined by Mann-Whitney non-parametric unpaired Student's t tests.

Article Snippet: Mouse monoclonal anti-human CD11b (clone ICRF44) , Fluidigm , Cat#3209003B RRID: AB_2687654.

Techniques: Marker, Expressing, Control, MANN-WHITNEY

Journal: iScience

Article Title: Peripheral immunophenotyping of AITD subjects reveals alterations in immune cells in pediatric vs adult-onset AITD

doi: 10.1016/j.isci.2021.103626

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-human CD11b (clone ICRF44) , Fluidigm , Cat#3209003B RRID: AB_2687654.

Techniques: Recombinant, Antibody Labeling, Immunopeptidomics, Mass Cytometry, Software

Journal: Journal for immunotherapy of cancer

Article Title: Subcutaneous checkpoint inhibition is equivalent to systemic delivery when combined with nelitolimod delivered via pressure-enabled drug delivery for depletion of intrahepatic myeloid-derived suppressor cells and control of liver metastases.

doi: 10.1136/jitc-2024-008837

Figure Lengend Snippet: Figure 2 Modulation of liver myeloid and lymphoid compartments by nelitolimod via pressure-enabled drug delivery was preserved in combination with Sys or SQ checkpoint inhibitor. Liver of tumor-bearing mice were harvested 10 days post- treatment. CD45+ cells were isolated from non-parenchymal cells. (A) MDSC cell population (CD11b+Gr1+), (B) monocytic MDSCs (M-MDSC; CD11b+Ly6C+/hiLy6G−/lo), (C) dendritic (CD11c+) cells, (D) B cells (B220+), (E) T cells (CD3+) and (F) M1- like macrophage (F/4/80+CD38+EGR2−) were quantified by flow cytometry. (G) i (a–h) Tumors were isolated from each group, OCT-mounted tissues were sectioned, fixed, and stained for CD3 (green), CD8 (red), CD11b (green), and Gr1 (red). (G) ii–iii Quantification of CD11b+Gr1+ MDSCs and CD3+CD8+T cells and from tumors of mice were performed across five fields/mouse and n=3 mice were used per group. Scale (20 µm). Animal data were presented as mean±SEM from and n was mentioned in the individual graph. One-way analysis of variance was performed to determine statistical differences among multiple groups. MDSC, myeloid-derived suppressor cells; M-MDSC, monocytic MDSC; G-MDSC, granulocytic MDSC; OCT, Optimal temperature cutting compound; DAPI, 4′,6-Diamidino-2-phenylindole; SQ, subcutaneous; Sys, systemic; Veh, vehicle.

Article Snippet: Primary antibodies and their corresponding clones used for immunofluorescence (IF) staining: CD11b; E6E1M (Cell Signaling, Massachusetts, USA), GR1; RB6- 8C5 (BioLegend, California, USA), CD3; SP7 (Novus Biologicals, USA) and CD8a; 53–6.7 μ (BioLegend, California, USA).

Techniques: Isolation, Flow Cytometry, Staining, Derivative Assay

Journal: Immunity

Article Title: Airway Epithelial Cell-Derived Colony Stimulating Factor-1 Promotes Allergen Sensitization

doi: 10.1016/j.immuni.2018.06.009

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Anti-human CD11b , Fluidigm , ICRF44, cat#3144001B.

Techniques: Antibody Labeling, Virus, Recombinant, Control, Enzyme-linked Immunosorbent Assay, Software