Journal: Nature Communications

Article Title: BAF60a deficiency uncouples chromatin accessibility and cold sensitivity from white fat browning

doi: 10.1038/s41467-020-16148-1

Figure Lengend Snippet: a qPCR analysis of gene expression during brown adipocyte differentiation following transduction of vector or Cre retrovirus ( n = 3 per group). b Immunoblots of brown adipocyte lysates during differentiation as in a . c qPCR analysis of differentiated brown adipocyte gene expression following 5 h of treatment with vehicle (Veh, n = 3, filled) or norepinephrine (NE, n = 3, open). d MitoTracker staining (scale bar = 100 μm; n = 3 per group). e Oxygen consumption rate (OCR) before and after FCCP addition ( n = 6 per group). Data in a , c , e represent mean ± s.d. ** P < 0.01, Vec vs. Cre; two-tailed unpaired Student’s t test. f Immunoblots of BAF60a-associated proteins in brown adipocytes (representative of three experiments). g Immunoblots of brown adipocyte lysates during differentiation in the presence of DMSO or 250 nM dBRD9 ( n = 2 per group). h qPCR analysis of Ucp1 expression ( n = 3 per group). i Immunoblots of brown adipocyte lysates. Differentiated adipocytes were treated with Veh or NE in the presence of DMSO, I-BRD9 (1 or 10 μM), or dBRD9 (100 or 500 nM) ( n = 2 per group). j qPCR analysis of Ucp1 expression in brown adipocytes treated with Veh or NE in the presence of DMSO, 10 μM I-BRD9 or 500 nM dBRD9 ( n = 4 per group). Data in h , j represent mean ± s.d. * P < 0.05, ** P < 0.01, DMSO vs. I-BRD9 or dBRD9; two-tailed unpaired Student’s t test. Source data are provided as a Source Data file.

Article Snippet: For NE stimulation, differentiated adipocytes were incubated with DMSO, I-BRD9 (Cayman), or dBRD9 for 6 h before the addition of saline or 1 μM NE for another 4 h (RNA) or 6 h (protein).

Techniques: Gene Expression, Transduction, Plasmid Preparation, Western Blot, Staining, Two Tailed Test, Expressing

Journal: Nature Communications

Article Title: BAF60a deficiency uncouples chromatin accessibility and cold sensitivity from white fat browning

doi: 10.1038/s41467-020-16148-1

Figure Lengend Snippet: a qPCR analysis of iWAT gene expression in mice following cold acclimation (flox, n = 6, filled; AKO, n = 9, open). Data represent mean ± s.e.m.; two-tailed unpaired Student’s t test, flox vs. AKO. b qPCR analysis of gene expression in differentiated beige adipocytes after treatments (5 h) with Veh, ACTH, KISS1, or Oxytocin (OXT) at two doses ( n = 4 per group). Two-tailed unpaired Student’s t test, treatments vs. Veh. c qPCR analysis of Mc2r expression in stromal vascular fraction (SVF) and mature adipocytes (Adipocyte) from iWAT of flox ( n = 4, filled) and AKO ( n = 3, open) mice. Data represent mean ± s.e.m. flox vs. AKO, two-tailed unpaired Student’s t test. d UCSC genome browser view of ATAC-seq peaks at the Mc2r locus. e qPCR analysis of Mc2r gene expression in beige adipocytes after 5 h treatments with DMSO or 1 μM rosiglitazone (Rosi) ( n = 4 per group). Data represent mean ± s.d. Rosi vs. DMSO, two-tailed unpaired Student’s t test. f Norepinephrine (NE) and epinephrine (Epi) levels in iWAT after chronic cold acclimation (flox, n = 10, filled; AKO, n = 10, open). Plasma ACTH levels at ambient temperature (flox n = 7; AKO, n = 6) or following cold acclimation (flox n = 6; AKO n = 7). Data represent mean ± s.e.m. Source data are provided as a Source Data file.

Article Snippet: For NE stimulation, differentiated adipocytes were incubated with DMSO, I-BRD9 (Cayman), or dBRD9 for 6 h before the addition of saline or 1 μM NE for another 4 h (RNA) or 6 h (protein).

Techniques: Gene Expression, Two Tailed Test, Expressing, Clinical Proteomics