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Image Search Results
Journal: bioRxiv
Article Title: ZEB2 transduces HIF1α dependent regulation of Transglutaminase 2 in glomerular podocytes
doi: 10.1101/2021.12.15.472753
Figure Lengend Snippet: (A) Expression of HIF1α, ZEB2, TG2 , and TRPC6 was analyzed between healthy volunteers (n=3) and chronic kidney disease patients (n=5) in the validated set group of Nakagawa CKD kidney database in Nephroseq ( www.nephroseq.org ). Immunoblotting analysis of HIF1α, TG2, and β-actin in human glomerular podocytes treated with (B) 1,5,10, and 15 µM of FG4592 for 12h and ( C ) 10µM FG4592 for 3,6,12, and 24h. (D) Immunofluorescence detection of TG2 in control vs. FG4592 treated podocytes. Scale bar 20μm. Nephroseq data was validated in human podocytes exposed to 10µM of FG4592 for 24h by (E) immunoblotting and ( F ) qRT-PCR. Error bars indicate mean ± SE; n=3. *, p < 0.02; **, p <0.001; ***, p < 0.0002; ****, p < 0.0001 by student t-test.
Article Snippet: TRPC6 siRNA (#SC-42672), ZEB2 siRNA (#SC-38641) and
Techniques: Expressing, Western Blot, Immunofluorescence, Control, Quantitative RT-PCR
Journal: bioRxiv
Article Title: ZEB2 transduces HIF1α dependent regulation of Transglutaminase 2 in glomerular podocytes
doi: 10.1101/2021.12.15.472753
Figure Lengend Snippet: Immunoblotting analysis of HIF1α, ZEB2,TRPC6,TG2, vimentin, fibronectin, and α-SMA in podocytes (A) treated with FG4592 in the presence or absence of metformin or (B) exposed to hypoxia (1% oxygen) in the presence or absence of metformin, and (C) exposed to hypoxia (1% oxygen) in the presence or absence of HIF1α inhibitor. Immunofluorescence detection of (D) HIF1α and (E) TG2 in podocytes treated with FG4592 with or without metformin treatment. Scale bar 10μm.
Article Snippet: TRPC6 siRNA (#SC-42672), ZEB2 siRNA (#SC-38641) and
Techniques: Western Blot, Immunofluorescence
Journal: bioRxiv
Article Title: ZEB2 transduces HIF1α dependent regulation of Transglutaminase 2 in glomerular podocytes
doi: 10.1101/2021.12.15.472753
Figure Lengend Snippet: (A) Immunoblotting analysis of HIF1α, ZEB2, TRPC6, TG2 and mesenchymal markers in glomerular lysate from mice administered with FG4592 and treated with or without metformin. (B) Masson’s Trichrome Staining (MTS), PAS, and H&E staining of glomerular regions of mice administered with FG4592 and treated with or without metformin. Scale bar 20μm. (C) Glomerular injury analysis was performed as described in the methods section. Data presented as mean ± SE; n=9. ***, p < 0.0005; ****, p < 0.0001 by one-way ANOVA after Tukey’s multiple comparison test. (D) TEM images of podocytes from mice administered with FG4592 and treated with or without metformin. Arrow marks indicate the podocyte FPE. Scale bar 1μm.
Article Snippet: TRPC6 siRNA (#SC-42672), ZEB2 siRNA (#SC-38641) and
Techniques: Western Blot, Staining, Comparison
Journal: bioRxiv
Article Title: ZEB2 transduces HIF1α dependent regulation of Transglutaminase 2 in glomerular podocytes
doi: 10.1101/2021.12.15.472753
Figure Lengend Snippet: (A) Silver staining of urinary proteins from mice administered with FG4592 and metformin. (B) UACR from mice administered with FG4592 and treated with or without metformin. Data presented as mean ± SE; n=6. ****, p < 0.0001 by one-way ANOVA after Tukey’s multiple comparison test. (C) Representative images of immunohistochemical staining for TG2 and ZEB2 in glomerular sections from people with or without DN. Scale bars indicate 20μm. (D) Albumin levels were estimated from the human urine samples in healthy vs DN patients. Data presented as mean ± SE; n=12. ****, p < 0.0001 by student t-test. (E) A scheme depicting the mechanism of regulation of TG2 by HIF1α/ZEB2 axis.
Article Snippet: TRPC6 siRNA (#SC-42672), ZEB2 siRNA (#SC-38641) and
Techniques: Silver Staining, Comparison, Immunohistochemical staining, Staining