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hif 1α  (Boster Bio)
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H. hathewayi -derived succinate upregulates <t>HIF-1α</t> and SUCNR1 expression and promotes metastasis by inducing EMT in HCT15 cells. ( A ) Relative mRNA expression of SUCNR1 in HCT15 cells treated with SA or HHM by qPCR. HCT15 cells were cultured in cell medium with 5% HHM, GAM, and SA (positive control) for 24 h. ( B ) Relative mRNA expression of HIF-1α in HCT15 cells treated with 1 mM SA or HHM by qPCR. Cells were cultured under conditions described in ( A ). Untreated cells served as the blank control. ( C – E ) Relative mRNA expression of CDH1 , Vimentin and Snail1 in HCT15 cells treated with SA or HHM by qPCR. Cells were cultured under conditions described in ( A ). Untreated cells served as the blank control. ( F ) Analysis of CDH1, HIF-1α, Snail1, and Vimentin protein expression in HCT15 cells by Western blot. HCT15 cells were cultured in cell medium with 5% HHM, GAM, and SA (positive control) for 24 h. HHM, the culture medium supernatant of H. hathewayi . GAM, bacterial culture medium. SA, 1 mM succinate. Data were from independent experiments and shown as mean ± SD. Compared using Student’s t -test between two groups, and differences among the groups were calculated using One Way ANOVA, followed by Dunnett’s multiple comparison test. Differences among the groups were calculated using One Way ANOVA. * p < 0.05, ** p < 0.01 and **** p < 0.0001.
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H. hathewayi -derived succinate upregulates <t>HIF-1α</t> and SUCNR1 expression and promotes metastasis by inducing EMT in HCT15 cells. ( A ) Relative mRNA expression of SUCNR1 in HCT15 cells treated with SA or HHM by qPCR. HCT15 cells were cultured in cell medium with 5% HHM, GAM, and SA (positive control) for 24 h. ( B ) Relative mRNA expression of HIF-1α in HCT15 cells treated with 1 mM SA or HHM by qPCR. Cells were cultured under conditions described in ( A ). Untreated cells served as the blank control. ( C – E ) Relative mRNA expression of CDH1 , Vimentin and Snail1 in HCT15 cells treated with SA or HHM by qPCR. Cells were cultured under conditions described in ( A ). Untreated cells served as the blank control. ( F ) Analysis of CDH1, HIF-1α, Snail1, and Vimentin protein expression in HCT15 cells by Western blot. HCT15 cells were cultured in cell medium with 5% HHM, GAM, and SA (positive control) for 24 h. HHM, the culture medium supernatant of H. hathewayi . GAM, bacterial culture medium. SA, 1 mM succinate. Data were from independent experiments and shown as mean ± SD. Compared using Student’s t -test between two groups, and differences among the groups were calculated using One Way ANOVA, followed by Dunnett’s multiple comparison test. Differences among the groups were calculated using One Way ANOVA. * p < 0.05, ** p < 0.01 and **** p < 0.0001.
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hif1α elisa kit  (Cusabio)
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Cusabio hif1α elisa kit
H. hathewayi -derived succinate upregulates <t>HIF-1α</t> and SUCNR1 expression and promotes metastasis by inducing EMT in HCT15 cells. ( A ) Relative mRNA expression of SUCNR1 in HCT15 cells treated with SA or HHM by qPCR. HCT15 cells were cultured in cell medium with 5% HHM, GAM, and SA (positive control) for 24 h. ( B ) Relative mRNA expression of HIF-1α in HCT15 cells treated with 1 mM SA or HHM by qPCR. Cells were cultured under conditions described in ( A ). Untreated cells served as the blank control. ( C – E ) Relative mRNA expression of CDH1 , Vimentin and Snail1 in HCT15 cells treated with SA or HHM by qPCR. Cells were cultured under conditions described in ( A ). Untreated cells served as the blank control. ( F ) Analysis of CDH1, HIF-1α, Snail1, and Vimentin protein expression in HCT15 cells by Western blot. HCT15 cells were cultured in cell medium with 5% HHM, GAM, and SA (positive control) for 24 h. HHM, the culture medium supernatant of H. hathewayi . GAM, bacterial culture medium. SA, 1 mM succinate. Data were from independent experiments and shown as mean ± SD. Compared using Student’s t -test between two groups, and differences among the groups were calculated using One Way ANOVA, followed by Dunnett’s multiple comparison test. Differences among the groups were calculated using One Way ANOVA. * p < 0.05, ** p < 0.01 and **** p < 0.0001.
Hif1α Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elabscience human elisa kit  (Elabscience Biotechnology)
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H. hathewayi -derived succinate upregulates <t>HIF-1α</t> and SUCNR1 expression and promotes metastasis by inducing EMT in HCT15 cells. ( A ) Relative mRNA expression of SUCNR1 in HCT15 cells treated with SA or HHM by qPCR. HCT15 cells were cultured in cell medium with 5% HHM, GAM, and SA (positive control) for 24 h. ( B ) Relative mRNA expression of HIF-1α in HCT15 cells treated with 1 mM SA or HHM by qPCR. Cells were cultured under conditions described in ( A ). Untreated cells served as the blank control. ( C – E ) Relative mRNA expression of CDH1 , Vimentin and Snail1 in HCT15 cells treated with SA or HHM by qPCR. Cells were cultured under conditions described in ( A ). Untreated cells served as the blank control. ( F ) Analysis of CDH1, HIF-1α, Snail1, and Vimentin protein expression in HCT15 cells by Western blot. HCT15 cells were cultured in cell medium with 5% HHM, GAM, and SA (positive control) for 24 h. HHM, the culture medium supernatant of H. hathewayi . GAM, bacterial culture medium. SA, 1 mM succinate. Data were from independent experiments and shown as mean ± SD. Compared using Student’s t -test between two groups, and differences among the groups were calculated using One Way ANOVA, followed by Dunnett’s multiple comparison test. Differences among the groups were calculated using One Way ANOVA. * p < 0.05, ** p < 0.01 and **** p < 0.0001.
Elabscience Human Elisa Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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atmosphere  (Plas-Labs inc)
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Plas-Labs inc atmosphere
H. hathewayi -derived succinate upregulates <t>HIF-1α</t> and SUCNR1 expression and promotes metastasis by inducing EMT in HCT15 cells. ( A ) Relative mRNA expression of SUCNR1 in HCT15 cells treated with SA or HHM by qPCR. HCT15 cells were cultured in cell medium with 5% HHM, GAM, and SA (positive control) for 24 h. ( B ) Relative mRNA expression of HIF-1α in HCT15 cells treated with 1 mM SA or HHM by qPCR. Cells were cultured under conditions described in ( A ). Untreated cells served as the blank control. ( C – E ) Relative mRNA expression of CDH1 , Vimentin and Snail1 in HCT15 cells treated with SA or HHM by qPCR. Cells were cultured under conditions described in ( A ). Untreated cells served as the blank control. ( F ) Analysis of CDH1, HIF-1α, Snail1, and Vimentin protein expression in HCT15 cells by Western blot. HCT15 cells were cultured in cell medium with 5% HHM, GAM, and SA (positive control) for 24 h. HHM, the culture medium supernatant of H. hathewayi . GAM, bacterial culture medium. SA, 1 mM succinate. Data were from independent experiments and shown as mean ± SD. Compared using Student’s t -test between two groups, and differences among the groups were calculated using One Way ANOVA, followed by Dunnett’s multiple comparison test. Differences among the groups were calculated using One Way ANOVA. * p < 0.05, ** p < 0.01 and **** p < 0.0001.
Atmosphere, supplied by Plas-Labs inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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H. hathewayi -derived succinate upregulates HIF-1α and SUCNR1 expression and promotes metastasis by inducing EMT in HCT15 cells. ( A ) Relative mRNA expression of SUCNR1 in HCT15 cells treated with SA or HHM by qPCR. HCT15 cells were cultured in cell medium with 5% HHM, GAM, and SA (positive control) for 24 h. ( B ) Relative mRNA expression of HIF-1α in HCT15 cells treated with 1 mM SA or HHM by qPCR. Cells were cultured under conditions described in ( A ). Untreated cells served as the blank control. ( C – E ) Relative mRNA expression of CDH1 , Vimentin and Snail1 in HCT15 cells treated with SA or HHM by qPCR. Cells were cultured under conditions described in ( A ). Untreated cells served as the blank control. ( F ) Analysis of CDH1, HIF-1α, Snail1, and Vimentin protein expression in HCT15 cells by Western blot. HCT15 cells were cultured in cell medium with 5% HHM, GAM, and SA (positive control) for 24 h. HHM, the culture medium supernatant of H. hathewayi . GAM, bacterial culture medium. SA, 1 mM succinate. Data were from independent experiments and shown as mean ± SD. Compared using Student’s t -test between two groups, and differences among the groups were calculated using One Way ANOVA, followed by Dunnett’s multiple comparison test. Differences among the groups were calculated using One Way ANOVA. * p < 0.05, ** p < 0.01 and **** p < 0.0001.

Journal: Microorganisms

Article Title: Hungatella hathewayi : A Tumor-Derived Bacterium Enriched in Colorectal Cancer Tissues and a Potential Diagnostic Biomarker

doi: 10.3390/microorganisms14030707

Figure Lengend Snippet: H. hathewayi -derived succinate upregulates HIF-1α and SUCNR1 expression and promotes metastasis by inducing EMT in HCT15 cells. ( A ) Relative mRNA expression of SUCNR1 in HCT15 cells treated with SA or HHM by qPCR. HCT15 cells were cultured in cell medium with 5% HHM, GAM, and SA (positive control) for 24 h. ( B ) Relative mRNA expression of HIF-1α in HCT15 cells treated with 1 mM SA or HHM by qPCR. Cells were cultured under conditions described in ( A ). Untreated cells served as the blank control. ( C – E ) Relative mRNA expression of CDH1 , Vimentin and Snail1 in HCT15 cells treated with SA or HHM by qPCR. Cells were cultured under conditions described in ( A ). Untreated cells served as the blank control. ( F ) Analysis of CDH1, HIF-1α, Snail1, and Vimentin protein expression in HCT15 cells by Western blot. HCT15 cells were cultured in cell medium with 5% HHM, GAM, and SA (positive control) for 24 h. HHM, the culture medium supernatant of H. hathewayi . GAM, bacterial culture medium. SA, 1 mM succinate. Data were from independent experiments and shown as mean ± SD. Compared using Student’s t -test between two groups, and differences among the groups were calculated using One Way ANOVA, followed by Dunnett’s multiple comparison test. Differences among the groups were calculated using One Way ANOVA. * p < 0.05, ** p < 0.01 and **** p < 0.0001.

Article Snippet: The membranes were incubated overnight at 4 °C with primary antibodies targeting NLRP3 (Cat No. 30109-1-AP, ProteinTech Group, Inc., Rosemont, IL, USA), IL-1β (516288, ZEN-BIOSCIENCE Co., Ltd., Chengdu, China), ASC (Cat No. 10500-1-AP, ProteinTech Group, Inc.), Caspase-1 (Cat No. 22915-1-AP, ProteinTech Group, Inc.), IL-18 (Cat No. 10663-1-AP, ProteinTech Group, Inc.), HIF-1α (Cat. PB9253, Boster Biological Technology Co., Ltd., Wuhan, China), CDH1 (Cat. PTM-6222, PTM BioLab Inc., Hangzhou, China), Vimentin (Cat. PTM-5376, PTM BioLab Inc.), Snail1 (Cat. 3879, Cell Signaling Technology, Inc., Danvers, MA, USA), and β-actin (Cat. PTM-5455, PTM BioLab Inc.), followed by a 1 h incubation with secondary antibodies (Cat No. SA00001-2, ProteinTech Group, Inc.) at room temperature.

Techniques: Derivative Assay, Expressing, Cell Culture, Positive Control, Control, Western Blot, Comparison