Journal: Advanced Science

Article Title: Nanozyme‐Engineered Probiotic Microneedle Patch for Chronic Diabetic Wound Therapy

doi: 10.1002/advs.202512127

Figure Lengend Snippet: Hypoxia alleviation, angiogenesis promotion, and cell migration enhancement by Pt‐M@ B. sub . a) Oxygen evolution capability of Pt‐M@ B. sub in H 2 O 2 solution under hypoxia conditions. b) Oxygen detection probe [Ru(dpp) 3 ]Cl 2 stained on HaCaT cells with different treatments and d) corresponding quantitative analysis. c) Oxygen detection probe [Ru(dpp) 3 ]Cl 2 stained on NIH‐3T3 cells with different treatments and e) corresponding quantitative analysis. f) Immunofluorescence staining images of HIF‐1α on HaCaT cells with different treatments and h) corresponding quantitative analysis. g) Immunofluorescence staining images of HIF‐1α on NIH‐3T3 cells with different treatments and i) corresponding quantitative analysis. j) Representative images of tube formation of HUVECs treated with different groups. k) Quantitative analysis of tube formation of HUVECs. l) Microscopy images and m) quantitative analysis of HaCaT cell migration under different treatments. Each point represents mean ± SD (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Antibodies against TNF‐α, IL‐6, and HIF‐1α were purchased from MCE (USA).

Techniques: Migration, Staining, Immunofluorescence, Microscopy

Journal: Scientific Reports

Article Title: Targeted hypoxia-inducible factor 1-alpha (HIF1A) stabilization during in vitro maturation of bovine cumulus-oocyte complexes increases blastocyst rates

doi: 10.1038/s41598-025-33894-8

Figure Lengend Snippet: Effects of Roxadustat-mediated HIF1A stabilization during IVM on oocyte maturation and embryo development. COCs were matured in vitro in the presence of Roxadustat at 25 µM (R25), 50 µM (R50), or 100 µM (R100), or without treatment (Ctrl). ( a ) Maturation rates, ( b ) cleavage rates, and ( c ) blastocyst rates were analyzed. One-way ANOVA showed a significant effect of treatment on maturation ( p = 0.02) and blastocyst rates ( p = 0.01), but not on cleavage ( p = 0.33). Dunnett’s post hoc test was used for comparisons to the control. ( d ) Developmental distribution of 40 COCs per group based on observed maturation, cleavage, and blastocyst rates. Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01.

Article Snippet: To stabilize HIF1A during IVM, we inhibited PHDs by supplementing the maturation medium (BO-IVM) with increasing dosages of Roxadustat (FG-4592, MedChemExpress, NJ, USA).

Techniques: In Vitro, Control

Journal: Scientific Reports

Article Title: Targeted hypoxia-inducible factor 1-alpha (HIF1A) stabilization during in vitro maturation of bovine cumulus-oocyte complexes increases blastocyst rates

doi: 10.1038/s41598-025-33894-8

Figure Lengend Snippet: Effects of Roxadustat treatment on HIF1A /HIF1A ( a , b ), PHD2 ( c ), and PCNA ( d ) expression in cumulus cells. Five groups were analyzed: immature (I), control-matured (Ctrl), and Roxadustat-treated (R25, R50, R100). ( a ) HIF1A mRNA expression was analyzed by one-way ANOVA ( p < 0.001), followed by Dunnett’s post hoc test. ( b-d ) Representative Western blots and quantification of HIF1A, PHD2 and PCNA. The Kruskal-Wallis test revealed significant differences between the groups for PHD2 ( p = 0.02) and PCNA ( p = 0.003), but not for HIF1A ( p = 0.41). Dunn’s multiple comparison test was performed as a post hoc analysis for the significant results. Data were normalized to the control to reduce variability arising from biological replicates of primary cells. Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001. SOD = standardized optical density related to ACTB.

Article Snippet: To stabilize HIF1A during IVM, we inhibited PHDs by supplementing the maturation medium (BO-IVM) with increasing dosages of Roxadustat (FG-4592, MedChemExpress, NJ, USA).

Techniques: Expressing, Control, Western Blot, Comparison

Journal: Scientific Reports

Article Title: Targeted hypoxia-inducible factor 1-alpha (HIF1A) stabilization during in vitro maturation of bovine cumulus-oocyte complexes increases blastocyst rates

doi: 10.1038/s41598-025-33894-8

Figure Lengend Snippet: Effects of HIF1A stabilization during IVC. COCs were cultured in vitro in the presence of Roxadustat at 25 µM (R25), 50 µM (R50), or 100 µM (R100), or without treatment (Ctrl). ( a ) Cleavage rates, and ( b ) blastocyst rates were evaluated. One-way ANOVA revealed no significant differences in cleavage ( p = 0.8), but a significant effect on blastocyst formation ( p < 0.001). Dunnett’s post hoc test was used to compare each treated group to the control. Data are presented as mean ± SEM. ** p < 0.01.

Article Snippet: To stabilize HIF1A during IVM, we inhibited PHDs by supplementing the maturation medium (BO-IVM) with increasing dosages of Roxadustat (FG-4592, MedChemExpress, NJ, USA).

Techniques: Cell Culture, In Vitro, Control