Journal: iScience

Article Title: Migration of human T cells can be differentially directed by electric fields depending on the extracellular microenvironment

doi: 10.1016/j.isci.2024.109746

Figure Lengend Snippet:

Article Snippet: Mouse IgG2a isotype control - FITC , Miltenyi Biotec , Cat#: 130-113-271 Lot: 5200908299.

Techniques: Recombinant, Control, Cell Isolation, Isolation, Software

Journal: FEBS Open Bio

Article Title: High glucose stimulates expression of aldosterone synthase ( CYP 11B2 ) and secretion of aldosterone in human adrenal cells

doi: 10.1002/2211-5463.12277

Figure Lengend Snippet: Effects of ARB s (A) and CCB s (B) on the high glucose‐induced CYP 11B2 transactivation. In (A), CYP 11B2 ‐H295R cells were incubated with either 100 mg·dL −1 d ‐glu (control), 450 mg·dL −1 d ‐glu ( d ‐glu), 450 mg·dL −1 d ‐glu plus 1 μmol·L −1 losartan ( d ‐glu + Los), 450 mg·dL −1 d ‐glu plus 1 μmol·L −1 valsartan ( d ‐glu + Val), 450 mg·dL −1 d ‐glu plus 1 μmol·L −1 olmesartan ( d ‐glu + Olm), or 450 mg·dL −1 d ‐glu plus 1 μmol·L −1 candesartan ( d ‐glu + Can) for 48 h. Data represent mean ± SEM ( n = 3), percentage of control. * P < 0.01, vs. control. In (B), CYP 11B2 ‐H295R cells were incubated with either 100 mg·dL −1 d ‐glu, 450 mg·dL −1 d ‐glu, 450 mg·dL −1 d ‐glu plus 0.01 μmol·L −1 amlodipine, 450 mg·dL −1 d ‐glu plus 0.1 μmol·L −1 amlodipine, 450 mg·dL −1 d ‐glu plus 1 μmol·L −1 amlodipine, 450 mg·dL −1 d ‐glu plus 0.01 μmol·L −1 benidipine, 450 mg·dL −1 d ‐glu plus 0.1 μmol·L −1 benidipine, 450 mg·dL −1 d ‐glu plus 1 μmol·L −1 benidipine, 450 mg·dL −1 d ‐glu plus 0.01 μmol·L −1 efonidipine, 450 mg·dL −1 d ‐glu plus 0.1 μmol·L −1 efonidipine, 450 mg·dL −1 d ‐glu plus 1 μmol·L −1 efonidipine, 450 mg·dL −1 d ‐glu plus 0.01 μmol·L −1 nifedipine, 450 mg·dL −1 d ‐glu plus 0.1 μmol·L −1 nifedipine, or 450 mg·dL −1 d ‐glu plus 1 μmol·L −1 nifedipine for 96 h. Data represent mean ± SEM ( n = 4), percentage of 100 mg·dL −1 d ‐glu. * P < 0.01, vs. 100 mg·dL −1 d ‐glu. ** P < 0.05, vs. 450 mg·dL −1 d ‐glu. *** P < 0.01, vs. 450 mg·dL −1 d ‐glu.

Article Snippet: Olmesartan (olmesartan medoxomil) was purchased from Toronto Research Chemicals (North York, Canada).

Techniques: Incubation, Control

Journal: International Journal of Nanomedicine

Article Title: Engineered Cancer Cell Membrane-Functionalized Metal-Organic Frameworks for Irinotecan/Curcumin Codelivery in Colorectal Cancer: Enhanced Efficacy and Mitigated Toxicity

doi: 10.2147/IJN.S558675

Figure Lengend Snippet: MZCC characterization. ( A ) UV−Vis absorbance spectra of ZIF-8, CPT-11, CCM, ZIC and ZICC. ( B ) FT-IR of CCM, CPT-11, ZIF-8, ZIC and ZICC. ( C ) XRD of CCM, ZIF-8, ZIC, ZICC and MZCC. ( D ) TEM image of ZIC, ZICC and MZCC. Scale bar: 100 nm. ( E ) Dynamic light scattering of ZIC, ZICC and MZCC. ( F ) Zeta potentials of ZIC, ZICC and MZCC. ( G ) SDS-PAGE of marker, MCM, ZICC and MZCC.

Article Snippet: Irinotecan was purchased from Aladdin Scientific Co., Ltd. (Shanghai, China).

Techniques: SDS Page, Marker

Journal: Frontiers in Pharmacology

Article Title: Contributions of UDP-Glucuronosyltransferases to Human Hepatic and Intestinal Metabolism of Ticagrelor and Inhibition of UGTs and Cytochrome P450 Enzymes by Ticagrelor and its Glucuronidated Metabolite

doi: 10.3389/fphar.2021.761814

Figure Lengend Snippet: CYPs-selective metabolic pathways inhibition mediated by ticagrelor-O-Glu.

Article Snippet: Reference standard of ticagrelor (purity 95.0%), 7-Ethyl-10-hydroxy-camptothecin (SN-38) glucuronide (purity 97%), chenodeoxycholic acid 24-acyl-β-D-glucuronide (purity 96%), N-Acetyl serotonin β-D-glucuronide (purity 97%), mycophenolic acid β-D-glucuronide (purity 96%), 3′-azide-3′-deoxythymidine β-D-glucuronide sodium salt (purity 97%), bupropion (purity 98%), hydroxy bupropion (purity >98%), amodiaquine (purity >95%), N-Desethyl amodiaquine hydrochloride (purity >95%), 4′-hydroxylation diclofenac (purity >95%), S-mephenytoin, S - 4 - hydroxy mephenytoin (purity >98%), dextromethorphan (purity >95%), dextrorphan tartrate salt (purity >98%), and furafylline (purity >95%, internal standard) were purchased from Toronto Research Chemicals (Toronto, ON, Canada).

Techniques: Inhibition

Journal: Frontiers in Pharmacology

Article Title: Contributions of UDP-Glucuronosyltransferases to Human Hepatic and Intestinal Metabolism of Ticagrelor and Inhibition of UGTs and Cytochrome P450 Enzymes by Ticagrelor and its Glucuronidated Metabolite

doi: 10.3389/fphar.2021.761814

Figure Lengend Snippet: Effects of ticagrelor-O-Glu on the activities of human CYPs enzymes. bupropion, amodiaquine, diclofenac, [S]-mephenytoin, dextromethorphan, and testosterone were used as the probe substrates for CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6 and CYP3A4, respectively. Each bar represents the mean ± S.D. of triple incubations. *, p < 0.05; ***, p < 0.001, ****, p < 0.0001. Each bar represents the mean ± S.D. of triple incubations.

Article Snippet: Reference standard of ticagrelor (purity 95.0%), 7-Ethyl-10-hydroxy-camptothecin (SN-38) glucuronide (purity 97%), chenodeoxycholic acid 24-acyl-β-D-glucuronide (purity 96%), N-Acetyl serotonin β-D-glucuronide (purity 97%), mycophenolic acid β-D-glucuronide (purity 96%), 3′-azide-3′-deoxythymidine β-D-glucuronide sodium salt (purity 97%), bupropion (purity 98%), hydroxy bupropion (purity >98%), amodiaquine (purity >95%), N-Desethyl amodiaquine hydrochloride (purity >95%), 4′-hydroxylation diclofenac (purity >95%), S-mephenytoin, S - 4 - hydroxy mephenytoin (purity >98%), dextromethorphan (purity >95%), dextrorphan tartrate salt (purity >98%), and furafylline (purity >95%, internal standard) were purchased from Toronto Research Chemicals (Toronto, ON, Canada).

Techniques: