Journal: BioMed Research International

Article Title: Quantitative Serum Proteomic Analysis of Essential Hypertension Using iTRAQ Technique

doi: 10.1155/2017/6761549

Figure Lengend Snippet: Verification of CTSG, HYAL1, KNG1, TGF- β 1, and BK in serum. (a) The levels of these candidate special proteins and downstream substances were determined by ELISA in the serum of the HC group ( n = 27) and EH group ( n = 27). (b) Western blots of KNG1 and CTSG were performed. p values were calculated with independent samples t -test and all of the five proteins were significantly differentially expressed between the two groups ( p = 0.000). Transferrin was applied as a loading control in western blot.

Article Snippet: Human CTSG ELISA kit and human HYAL1 ELISA kit (Cusabio Biotech, Wuhan, Hubei, China), TGF- β 1, and KNG1 (Abcam, Cambridge, MA, USA) were applied to determine the concentration of these proteins in each serum of validation set (27 HC and 27 EH individuals).

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Control

Journal: American Journal of Respiratory Cell and Molecular Biology

Article Title: Polyinosine-Polycytidylic Acid Stimulates Versican Accumulation in the Extracellular Matrix Promoting Monocyte Adhesion

doi: 10.1165/rcmb.2009-0081oc

Figure Lengend Snippet: Figure 6. (A) Pulse–chase analysis of [3H]-labeled hyaluronan shows reduced turnover in poly I:C–treated cells. HLFs were metabolically labeled with tritiated glucosamine for 24 hours in the presence of 10% serum–containing medium alone or with addition of IL-1b plus TNF-a or 20 mg/ml poly I:C, as in Figure 5B, and then chased with fresh 0.1% FBS medium. Remaining radioactivity was normalized to the amount in the cell layers at time zero of the chase period. Closed circles, controls; open circles, poly I:C; open triangles, IL-1b plus TNF-a. The initial rate of degradation was much greater in controls and in IL-1b plus TNF- a– than in poly I:C–treated cells, whereas a second, more gradual, rate appeared to be similar for both control and poly I:C–treated cells. This experiment was performed twice with similar results. (B) mRNAs associated with hyaluronan degradation are decreased by poly I:C treatment. Cells were arrested in low-serum–containing medium, and then stimulated with IL-1b plus TNF-a, poly I:C, or no addition in 10% serum–containing medium. The cells then were harvested for mRNA analysis after 6-hour incubation. CD44, HYAL1, and HYAL2 mRNAs were significantly decreased in poly I:C–treated cells in comparison with controls. There was also a small, but significant, decrease in HYAL2 mRNA in IL-1b plus TNF-a–treated cells. Reduction in these degradative enzymes and in CD44, the cell surface receptor for hyaluronan, may help to explain the slower release of radiolabeled hyaluronan from poly I:C–treated cells. In contrast, mRNA for CD44 was increased over twofold in IL-1b plus TNF-a–treated cells. Open bars, IL-1b plus TNF-a; closed bars, poly I:C. *P , 0.05, **P , 0.01 compared with control. This experiment was performed three times with similar results.

Article Snippet: ABI gene expression assays used were as follows: hyaluronan synthase (HAS)–1 Hs00758053_m1; HAS-2 Hs00193435_m1; HAS-3 Hs00193436_m1; CD44 Hs00153304_m1; Hyal 1 Hs00537920_g1; Hyal 2 Hs00186841_m1; ADAMTS1 Hs00199608_m1; ADAMTS4 Hs00192708_m1; ADAMTS5 Hs00199841_m1; total versican Hs00171642_m1; versican V0 Hs01007944_m1; versican V1 Hs01007937_m1; versican V2 Hs01007943_m1; versican V3 Hs01007941_m1; 18S Hs99999901_s1; human TBP Hs99999910_m1.

Techniques: Pulse Chase, Labeling, Metabolic Labelling, Radioactivity, Control, Incubation, Comparison, Cell Surface Receptor Assay