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Image Search Results
Journal: BMC Plant Biology
Article Title: Comparative analysis of expressed sequence tags (ESTs) between drought-tolerant and -susceptible genotypes of chickpea under terminal drought stress
doi: 10.1186/1471-2229-11-70
Figure Lengend Snippet: Summary of drought responsive SSH libraries and ESTs.
Article Snippet: In our study, different LEA groups of genes were found in UG-TDS: 2
Techniques: Clone Assay
Journal: BMC Plant Biology
Article Title: Comparative analysis of expressed sequence tags (ESTs) between drought-tolerant and -susceptible genotypes of chickpea under terminal drought stress
doi: 10.1186/1471-2229-11-70
Figure Lengend Snippet: Venn diagram representing comparison of ESTs from different SSH libraries : (A) Cap3 assembly of four SSH libraries AS1-1(forward subtracted library from the shoots of drought tolerant genotype, ICC 4958), AR1-1(forward subtracted library from the roots of drought tolerant genotype, ICC 4958), containing up- regulated transcripts and AS2-1(reverse subtracted library from the shoots of drought tolerant genotype, ICC 4958), AR2-1(reverse subtracted library from the roots of drought tolerant genotype, ICC 4958) containing down regulated transcripts under TDS, reveals a set of 592 and 876unigenes specific to up- regulated and, down regulated libraries respectively. A set of 125 unigenes were common in both group. (B) ESTs obtained from bulk of RILs libraries Bulk 1-1(forward subtracted library from the roots of HRB and LRB) and Bulk 2-1(reverse subtracted library from the roots of HRB and LRB) and individual parental libraries, AB1-1 (forward subtracted library from the roots of ICC 4958 and ICC 1882)and AB2-1(reverse subtracted library from the roots of ICC 4958 and ICC 1882 ) reveals 343, 399, 262 and 298 unigenes specific to AB1-1, AB2-1, Bulk 1-1 and Bulk2-1 libraries, respectively.
Article Snippet: In our study, different LEA groups of genes were found in UG-TDS: 2
Techniques: Comparison
Journal: BMC Plant Biology
Article Title: Comparative analysis of expressed sequence tags (ESTs) between drought-tolerant and -susceptible genotypes of chickpea under terminal drought stress
doi: 10.1186/1471-2229-11-70
Figure Lengend Snippet: Distribution of conservation between chickpea ( Cicer arietinum ) UG-TDS and the EST datasets of Mt ( Medicago Truncatula ), Gm ( Glycine max ), Pv ( Phaseolus vulgaris ), Lj ( Lotus japonicus ), Pa ( Populus alba ), Os ( Oryza Sativa ) and At ( Arabidopsis thaliana ) . Unigenes were grouped according to similarity levels determined by nucleotide similarity search BLASTN E-value.
Article Snippet: In our study, different LEA groups of genes were found in UG-TDS: 2
Techniques:
Journal: BMC Plant Biology
Article Title: Comparative analysis of expressed sequence tags (ESTs) between drought-tolerant and -susceptible genotypes of chickpea under terminal drought stress
doi: 10.1186/1471-2229-11-70
Figure Lengend Snippet: A graphical representation of the annotation statistics of UG-TDS : the total number of unigenes annotated as a known protein with an E-value threshold of e-06, total number of unigens not mapped, total number of unigenes mapped but not annotated, the total number of unigene annotated with at least one category of Gene Ontology (GO) and the number of genes annotated in each of the 3 major GO categories, biological process, molecular function and cellular component.
Article Snippet: In our study, different LEA groups of genes were found in UG-TDS: 2
Techniques:
Journal: BMC Plant Biology
Article Title: Comparative analysis of expressed sequence tags (ESTs) between drought-tolerant and -susceptible genotypes of chickpea under terminal drought stress
doi: 10.1186/1471-2229-11-70
Figure Lengend Snippet: Heat map of expression values of drought responsive genes in ICC 4958 and ICC 1882 under TDS : Hierarchical clustering (average linkage and Euclidean distance matrix with the minimum similarity of 0.5) were performed using HCE version 2.0 beta web tool. Clustering of unigenes based on normalized signal intensity into three clusters (I, II, and III). The dendrogram of the array experiments reflects the similarity of the unigenes with respect to their gene-expression pattern. In the heat map red represents normalized expression values greater than the mean, green colour represents expression less than the mean and colour intensities in between the two represent the magnitude of the deviation from the mean. Colour scale (from green to red) represents the range of expression level.
Article Snippet: In our study, different LEA groups of genes were found in UG-TDS: 2
Techniques: Expressing, Gene Expression
Journal: BMC Plant Biology
Article Title: Comparative analysis of expressed sequence tags (ESTs) between drought-tolerant and -susceptible genotypes of chickpea under terminal drought stress
doi: 10.1186/1471-2229-11-70
Figure Lengend Snippet: Comparative expression analysis of selected unigenes between ICC 4958 and ICC 1882 chickpea genotypes in response to drought stress . Relative expression levels (fold difference) of 10 selected unigens in ICC 4958 and ICC 1882 chickpea genotypes under terminal drought stress were evaluated using qPCR analysis. Error bars represent Standard error of the mean (Number of replication n = 3). Unigenes used for qPCR analysis were: 1-aminocyclopropane-1-carboxylate synthase ( HO062180 ), esterase lipase thioesterase family protein-1( HO062244 ), yippee family protein (Putative zinc binding protein) ( HO062242 ), calcium ion binding ( HO062250 ), protein kinase ( HO062281 ), MADS box protein ( HO062366 ), esterase lipase thioesterase family protein-2 ( HO062386 ), alkaline alpha galactosidase ( HO062433 ), leucine-rich repeat protein ( HO062474 ) and GDP dissociation inhibitor ( HO062555 ).
Article Snippet: In our study, different LEA groups of genes were found in UG-TDS: 2
Techniques: Expressing, Binding Assay
Journal: Oxidative Medicine and Cellular Longevity
Article Title: Salidroside Protects Dopaminergic Neurons by Enhancing PINK1/Parkin-Mediated Mitophagy
doi: 10.1155/2019/9341018
Figure Lengend Snippet: Effect of Sal on MPTP-induced DA neuron damage. Representative photomicrographs and quantitative analysis of DAT-positive neurons in the striatum (a) and TH-positive neurons in the SN (b). Scale bar, 50 μ m. (c) Western blot analysis of DAT expression in the striatum and TH expression in the SN of mice. β -Actin served as loading controls. (d) Qualification analysis of protein expression of DAT and TH in mice. (e) Effect of Sal on the level of dopamine, DOPAC, and HVA in the striatum of mice. Each column represents the mean ± SD ( n = 3). ∗ P < 0.05 and ∗∗ P < 0.01, compared with the control group; # P < 0.05 and ## P < 0.01, compared with the MPTP-treated group.
Article Snippet: The dopamine, DOPAC, and
Techniques: Western Blot, Expressing