Journal:

Article Title: Increased RNA-Induced Silencing Complex (RISC) Activity Contributes to Hepatocellular Carcinoma

doi: 10.1002/hep.24216

Figure Lengend Snippet: Inhibition of SND1 enzymatic activity inhibits cell viability and AEG-1 function. A. Hep-pc-4 and Hep-AEG-1-14 cells were treated or not with 50, 100 and 200 μM pdTp for 1 to 4 days and cell viability was measured by standard MTT assay. B. Hep-pc-4, Hep-AEG-1-14, Hep3B and QGY-7703 cells were treated with 100 μM pdTp and colony formation assay was performed. The colonies were scored after two weeks. Data represent mean± SEM of three independent experiments. C. Analysis of SND1 expression in tissue microarray by immunohistochemistry. ˆ: p<0.05, #: p<0.02 and *: p<0.01 vs the corresponding data point in Hep-pc-4 cells.

Article Snippet: Tissue microarray and immunostaining Human HCC tissue microarrays were obtained from Imgenex Corp. Two tissue microarrays were used: one containing 40 primary HCC, 10 metastatic HCC and 9 normal adjacent liver samples (Imgenex; IMH-360), the other containing 46 primary HCC and 13 metastatic HCC (Imgenex; IMH-318).

Techniques: Inhibition, Activity Assay, MTT Assay, Colony Assay, Expressing, Microarray, Immunohistochemistry

Journal:

Article Title: Increased RNA-Induced Silencing Complex (RISC) Activity Contributes to Hepatocellular Carcinoma

doi: 10.1002/hep.24216

Figure Lengend Snippet: Immunoperoxidase staining of normal liver and different stages of HCC by tissue microarray using anti-SND1 antibody

Article Snippet: Tissue microarray and immunostaining Human HCC tissue microarrays were obtained from Imgenex Corp. Two tissue microarrays were used: one containing 40 primary HCC, 10 metastatic HCC and 9 normal adjacent liver samples (Imgenex; IMH-360), the other containing 46 primary HCC and 13 metastatic HCC (Imgenex; IMH-318).

Techniques: Immunoperoxidase Staining, Microarray, Staining