human spp1 dost00 Search Results


95
R&D Systems spp1
Identification of CD59 as a human-specific biomarker for the proximal nephron. ( A ). Schematic of the screening approach: normal human urinary proteome, in vitro cultured kidney organoid transcriptome, and transcriptome of engrafted kidney organoids were cross-referenced for common proteins/transcripts. ( B ). Amino acid identities of human versus mouse paralogs and results of measurement of human versus mouse using commercial ELISA kits. ( C ). Expression of <t>SPP1</t> in adult human kidney. ( D ). Expression of CD59 in adult human kidney. ( E ). Results of ELISA measurement of 3 male and 3 female urine samples from humans and mice. #NUM! indicates a measurement below the threshold of detection, 3.21 × 10 4 is the Glomax plate reader convention for 3.21 × 10 4 . ( F ). Schematic showing the origin of CD59 secretion (green) and uromodulin (THP secretion (purple).
Spp1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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99
Bio-Techne corporation 1031
Identification of CD59 as a human-specific biomarker for the proximal nephron. ( A ). Schematic of the screening approach: normal human urinary proteome, in vitro cultured kidney organoid transcriptome, and transcriptome of engrafted kidney organoids were cross-referenced for common proteins/transcripts. ( B ). Amino acid identities of human versus mouse paralogs and results of measurement of human versus mouse using commercial ELISA kits. ( C ). Expression of <t>SPP1</t> in adult human kidney. ( D ). Expression of CD59 in adult human kidney. ( E ). Results of ELISA measurement of 3 male and 3 female urine samples from humans and mice. #NUM! indicates a measurement below the threshold of detection, 3.21 × 10 4 is the Glomax plate reader convention for 3.21 × 10 4 . ( F ). Schematic showing the origin of CD59 secretion (green) and uromodulin (THP secretion (purple).
1031, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Abnova human apoe ka 1031
Identification of CD59 as a human-specific biomarker for the proximal nephron. ( A ). Schematic of the screening approach: normal human urinary proteome, in vitro cultured kidney organoid transcriptome, and transcriptome of engrafted kidney organoids were cross-referenced for common proteins/transcripts. ( B ). Amino acid identities of human versus mouse paralogs and results of measurement of human versus mouse using commercial ELISA kits. ( C ). Expression of <t>SPP1</t> in adult human kidney. ( D ). Expression of CD59 in adult human kidney. ( E ). Results of ELISA measurement of 3 male and 3 female urine samples from humans and mice. #NUM! indicates a measurement below the threshold of detection, 3.21 × 10 4 is the Glomax plate reader convention for 3.21 × 10 4 . ( F ). Schematic showing the origin of CD59 secretion (green) and uromodulin (THP secretion (purple).
Human Apoe Ka 1031, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Abnova human apoe
Identification of CD59 as a human-specific biomarker for the proximal nephron. ( A ). Schematic of the screening approach: normal human urinary proteome, in vitro cultured kidney organoid transcriptome, and transcriptome of engrafted kidney organoids were cross-referenced for common proteins/transcripts. ( B ). Amino acid identities of human versus mouse paralogs and results of measurement of human versus mouse using commercial ELISA kits. ( C ). Expression of <t>SPP1</t> in adult human kidney. ( D ). Expression of CD59 in adult human kidney. ( E ). Results of ELISA measurement of 3 male and 3 female urine samples from humans and mice. #NUM! indicates a measurement below the threshold of detection, 3.21 × 10 4 is the Glomax plate reader convention for 3.21 × 10 4 . ( F ). Schematic showing the origin of CD59 secretion (green) and uromodulin (THP secretion (purple).
Human Apoe, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Promega glomax explorer
Identification of CD59 as a human-specific biomarker for the proximal nephron. ( A ). Schematic of the screening approach: normal human urinary proteome, in vitro cultured kidney organoid transcriptome, and transcriptome of engrafted kidney organoids were cross-referenced for common proteins/transcripts. ( B ). Amino acid identities of human versus mouse paralogs and results of measurement of human versus mouse using commercial ELISA kits. ( C ). Expression of <t>SPP1</t> in adult human kidney. ( D ). Expression of CD59 in adult human kidney. ( E ). Results of ELISA measurement of 3 male and 3 female urine samples from humans and mice. #NUM! indicates a measurement below the threshold of detection, 3.21 × 10 4 is the Glomax plate reader convention for 3.21 × 10 4 . ( F ). Schematic showing the origin of CD59 secretion (green) and uromodulin (THP secretion (purple).
Glomax Explorer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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92
Cusabio spon1
Correlation of PEA with SOMAscan data. (A) Data for 107 markers were analyzed to calculate the cumulative distribution of Spearman correlation coefficients (ρ) between PEA and SOMAscan signal intensities, yielding a median value of ρ = 0.62. The analysis was carried out with 20 OC-plasma and 10 N-plasma samples. The light blue area indicates positive correlations (85.05% of all instances), light red indicates negative correlations (14.95%). (B) Dot plots showing highly significant positive correlations of PEA and SOMAscan data ( n = 30) for KLK11, MMP9, <t>SPON1,</t> and SPP1/OPN.
Spon1, supplied by Cusabio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Boster Bio spint2
Cellular origin of upregulated proteins. (A) Transcriptome analysis of the top 30 proteins increased in OC-plasma (from ). (B) Proteome analysis as in (A) . Due to the lower sensitivity of MS-based proteomics, especially for secreted proteins , data were not available for a number of proteins. Boxplots show medians (horizontal line in boxes), upper and lower quartiles (box) and range (whiskers). Arrows point out MUC16, <t>SPINT2,</t> and WFDC2. TU, tumor cells; TAM, tumor-associated macrophage; TAT, tumor-associated T-cells.
Spint2, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Bruker Corporation proteinscape 2.1
Cellular origin of upregulated proteins. (A) Transcriptome analysis of the top 30 proteins increased in OC-plasma (from ). (B) Proteome analysis as in (A) . Due to the lower sensitivity of MS-based proteomics, especially for secreted proteins , data were not available for a number of proteins. Boxplots show medians (horizontal line in boxes), upper and lower quartiles (box) and range (whiskers). Arrows point out MUC16, <t>SPINT2,</t> and WFDC2. TU, tumor cells; TAM, tumor-associated macrophage; TAT, tumor-associated T-cells.
Proteinscape 2.1, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
STEMCELL Technologies Inc easysep human monocyte enrichment kit
Cellular origin of upregulated proteins. (A) Transcriptome analysis of the top 30 proteins increased in OC-plasma (from ). (B) Proteome analysis as in (A) . Due to the lower sensitivity of MS-based proteomics, especially for secreted proteins , data were not available for a number of proteins. Boxplots show medians (horizontal line in boxes), upper and lower quartiles (box) and range (whiskers). Arrows point out MUC16, <t>SPINT2,</t> and WFDC2. TU, tumor cells; TAM, tumor-associated macrophage; TAT, tumor-associated T-cells.
Easysep Human Monocyte Enrichment Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Jackson Laboratory mouse: opn / (rd8 mutation free)
Cellular origin of upregulated proteins. (A) Transcriptome analysis of the top 30 proteins increased in OC-plasma (from ). (B) Proteome analysis as in (A) . Due to the lower sensitivity of MS-based proteomics, especially for secreted proteins , data were not available for a number of proteins. Boxplots show medians (horizontal line in boxes), upper and lower quartiles (box) and range (whiskers). Arrows point out MUC16, <t>SPINT2,</t> and WFDC2. TU, tumor cells; TAM, tumor-associated macrophage; TAT, tumor-associated T-cells.
Mouse: Opn / (Rd8 Mutation Free), supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Jackson Laboratory mouse: thbs1 / (rd8 mutation free)
Cellular origin of upregulated proteins. (A) Transcriptome analysis of the top 30 proteins increased in OC-plasma (from ). (B) Proteome analysis as in (A) . Due to the lower sensitivity of MS-based proteomics, especially for secreted proteins , data were not available for a number of proteins. Boxplots show medians (horizontal line in boxes), upper and lower quartiles (box) and range (whiskers). Arrows point out MUC16, <t>SPINT2,</t> and WFDC2. TU, tumor cells; TAM, tumor-associated macrophage; TAT, tumor-associated T-cells.
Mouse: Thbs1 / (Rd8 Mutation Free), supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Millipore milliplex map human cytokine/chemokine magnetic bead panels
Cellular origin of upregulated proteins. (A) Transcriptome analysis of the top 30 proteins increased in OC-plasma (from ). (B) Proteome analysis as in (A) . Due to the lower sensitivity of MS-based proteomics, especially for secreted proteins , data were not available for a number of proteins. Boxplots show medians (horizontal line in boxes), upper and lower quartiles (box) and range (whiskers). Arrows point out MUC16, <t>SPINT2,</t> and WFDC2. TU, tumor cells; TAM, tumor-associated macrophage; TAT, tumor-associated T-cells.
Milliplex Map Human Cytokine/Chemokine Magnetic Bead Panels, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Identification of CD59 as a human-specific biomarker for the proximal nephron. ( A ). Schematic of the screening approach: normal human urinary proteome, in vitro cultured kidney organoid transcriptome, and transcriptome of engrafted kidney organoids were cross-referenced for common proteins/transcripts. ( B ). Amino acid identities of human versus mouse paralogs and results of measurement of human versus mouse using commercial ELISA kits. ( C ). Expression of SPP1 in adult human kidney. ( D ). Expression of CD59 in adult human kidney. ( E ). Results of ELISA measurement of 3 male and 3 female urine samples from humans and mice. #NUM! indicates a measurement below the threshold of detection, 3.21 × 10 4 is the Glomax plate reader convention for 3.21 × 10 4 . ( F ). Schematic showing the origin of CD59 secretion (green) and uromodulin (THP secretion (purple).

Journal: Bioengineering

Article Title: In Vivo Assessment of Laboratory-Grown Kidney Tissue Grafts

doi: 10.3390/bioengineering10111261

Figure Lengend Snippet: Identification of CD59 as a human-specific biomarker for the proximal nephron. ( A ). Schematic of the screening approach: normal human urinary proteome, in vitro cultured kidney organoid transcriptome, and transcriptome of engrafted kidney organoids were cross-referenced for common proteins/transcripts. ( B ). Amino acid identities of human versus mouse paralogs and results of measurement of human versus mouse using commercial ELISA kits. ( C ). Expression of SPP1 in adult human kidney. ( D ). Expression of CD59 in adult human kidney. ( E ). Results of ELISA measurement of 3 male and 3 female urine samples from humans and mice. #NUM! indicates a measurement below the threshold of detection, 3.21 × 10 4 is the Glomax plate reader convention for 3.21 × 10 4 . ( F ). Schematic showing the origin of CD59 secretion (green) and uromodulin (THP secretion (purple).

Article Snippet: Assays for uromodulin (Human Uromodulin DuoSet ELISA DY5144-05, R&D Systems, Minneapolis, MN, USA), CD59 (Human CD59 ELISA ab263893, Abcam, Waltham, MA, USA), PSAP (Human PSAP/Prosaposin (Sandwich ELISA) ELISA LS-F35235, Lifespan Biosciences, Lynnwood, MA, USA), cubilin (Human CUBN/Cubilin (Sandwich ELISA) ELISA LS-F38077, Lifespan Biosciences, Lynnwood, MA, USA), SPP1 (Human Osteopontin (OPN) Quantikine ELISA DOST00, R&D Systems, Minneapolis, MN, USA), and HSPG2 (HSPG2 elisa kit: Human Basement membrane-specific heparan sulfate proteoglycan core protein ELISA MBS765938, MyBioSource, San Diego, CA, USA) were used according to the manufacturer’s instructions and assays were read on a Glomax Explorer (Promega, Madison, WI, USA) plate reader.

Techniques: Biomarker Discovery, In Vitro, Cell Culture, Enzyme-linked Immunosorbent Assay, Expressing

Correlation of PEA with SOMAscan data. (A) Data for 107 markers were analyzed to calculate the cumulative distribution of Spearman correlation coefficients (ρ) between PEA and SOMAscan signal intensities, yielding a median value of ρ = 0.62. The analysis was carried out with 20 OC-plasma and 10 N-plasma samples. The light blue area indicates positive correlations (85.05% of all instances), light red indicates negative correlations (14.95%). (B) Dot plots showing highly significant positive correlations of PEA and SOMAscan data ( n = 30) for KLK11, MMP9, SPON1, and SPP1/OPN.

Journal: Frontiers in Oncology

Article Title: Multi-platform Affinity Proteomics Identify Proteins Linked to Metastasis and Immune Suppression in Ovarian Cancer Plasma

doi: 10.3389/fonc.2019.01150

Figure Lengend Snippet: Correlation of PEA with SOMAscan data. (A) Data for 107 markers were analyzed to calculate the cumulative distribution of Spearman correlation coefficients (ρ) between PEA and SOMAscan signal intensities, yielding a median value of ρ = 0.62. The analysis was carried out with 20 OC-plasma and 10 N-plasma samples. The light blue area indicates positive correlations (85.05% of all instances), light red indicates negative correlations (14.95%). (B) Dot plots showing highly significant positive correlations of PEA and SOMAscan data ( n = 30) for KLK11, MMP9, SPON1, and SPP1/OPN.

Article Snippet: Other proteins were quantified by ELISA according to the instructions of the respective manufacturer: BCAM (ELH-BCAM-2; BioCat GmbH, Heidelberg, Germany); EPHA2 (ELH-EPHA2-1; RayBiotech Life, Peachtree Corners, GA, USA); GDF15 (DGD150; R&D Systems, Wiesbaden, Germany); IL-6 (Invitrogen-88-7066-22; Thermo Fisher Scientific, Schwerte, Germany); IL-18BP (DBP180; R&D Systems, Wiesbaden, Germany); OPN/SPP1 (DOST00; R&D Systems, Wiesbaden, Germany); SPON1 (CSB-EL022599HU-96; Cusabio, Houston, TX, USA); VEGFA (BMS277-2; Thermo Fisher Scientific, Schwerte, Germany); WFDC2/HE4 (DHE400; R&D Systems, Wiesbaden, Germany); SPINT2 (EK0773-CAP; Boster, Pleasanton, USA); PVRL4/NECTIN4 (DNEC40; R&D Systems, Wiesbaden, Germany).

Techniques: Clinical Proteomics

Cellular origin of upregulated proteins. (A) Transcriptome analysis of the top 30 proteins increased in OC-plasma (from ). (B) Proteome analysis as in (A) . Due to the lower sensitivity of MS-based proteomics, especially for secreted proteins , data were not available for a number of proteins. Boxplots show medians (horizontal line in boxes), upper and lower quartiles (box) and range (whiskers). Arrows point out MUC16, SPINT2, and WFDC2. TU, tumor cells; TAM, tumor-associated macrophage; TAT, tumor-associated T-cells.

Journal: Frontiers in Oncology

Article Title: Multi-platform Affinity Proteomics Identify Proteins Linked to Metastasis and Immune Suppression in Ovarian Cancer Plasma

doi: 10.3389/fonc.2019.01150

Figure Lengend Snippet: Cellular origin of upregulated proteins. (A) Transcriptome analysis of the top 30 proteins increased in OC-plasma (from ). (B) Proteome analysis as in (A) . Due to the lower sensitivity of MS-based proteomics, especially for secreted proteins , data were not available for a number of proteins. Boxplots show medians (horizontal line in boxes), upper and lower quartiles (box) and range (whiskers). Arrows point out MUC16, SPINT2, and WFDC2. TU, tumor cells; TAM, tumor-associated macrophage; TAT, tumor-associated T-cells.

Article Snippet: Other proteins were quantified by ELISA according to the instructions of the respective manufacturer: BCAM (ELH-BCAM-2; BioCat GmbH, Heidelberg, Germany); EPHA2 (ELH-EPHA2-1; RayBiotech Life, Peachtree Corners, GA, USA); GDF15 (DGD150; R&D Systems, Wiesbaden, Germany); IL-6 (Invitrogen-88-7066-22; Thermo Fisher Scientific, Schwerte, Germany); IL-18BP (DBP180; R&D Systems, Wiesbaden, Germany); OPN/SPP1 (DOST00; R&D Systems, Wiesbaden, Germany); SPON1 (CSB-EL022599HU-96; Cusabio, Houston, TX, USA); VEGFA (BMS277-2; Thermo Fisher Scientific, Schwerte, Germany); WFDC2/HE4 (DHE400; R&D Systems, Wiesbaden, Germany); SPINT2 (EK0773-CAP; Boster, Pleasanton, USA); PVRL4/NECTIN4 (DNEC40; R&D Systems, Wiesbaden, Germany).

Techniques: Clinical Proteomics

Association of SPINT2 mRNA expression with survival of OC patients. (A) Kaplan-Meier plot for 1074 HGSC patients in the Kaplan–Meier Plotter database (updated version at http://kmplot.com ) analyzing the association of SPINT2 with RFS. HR, hazard ratio. (B) Kaplan-Meier plot for 1074 HGSC patients in the same database analyzing the association of SPINT2 with OS. (C) z-scores (PRECOG data) for the association of SPINT2 with the overall survival (OS) of the indicated tumor entities . Red: association with a short OS (z-score above +1.5;). Blue: association with a short OS (z-score below −1.5).

Journal: Frontiers in Oncology

Article Title: Multi-platform Affinity Proteomics Identify Proteins Linked to Metastasis and Immune Suppression in Ovarian Cancer Plasma

doi: 10.3389/fonc.2019.01150

Figure Lengend Snippet: Association of SPINT2 mRNA expression with survival of OC patients. (A) Kaplan-Meier plot for 1074 HGSC patients in the Kaplan–Meier Plotter database (updated version at http://kmplot.com ) analyzing the association of SPINT2 with RFS. HR, hazard ratio. (B) Kaplan-Meier plot for 1074 HGSC patients in the same database analyzing the association of SPINT2 with OS. (C) z-scores (PRECOG data) for the association of SPINT2 with the overall survival (OS) of the indicated tumor entities . Red: association with a short OS (z-score above +1.5;). Blue: association with a short OS (z-score below −1.5).

Article Snippet: Other proteins were quantified by ELISA according to the instructions of the respective manufacturer: BCAM (ELH-BCAM-2; BioCat GmbH, Heidelberg, Germany); EPHA2 (ELH-EPHA2-1; RayBiotech Life, Peachtree Corners, GA, USA); GDF15 (DGD150; R&D Systems, Wiesbaden, Germany); IL-6 (Invitrogen-88-7066-22; Thermo Fisher Scientific, Schwerte, Germany); IL-18BP (DBP180; R&D Systems, Wiesbaden, Germany); OPN/SPP1 (DOST00; R&D Systems, Wiesbaden, Germany); SPON1 (CSB-EL022599HU-96; Cusabio, Houston, TX, USA); VEGFA (BMS277-2; Thermo Fisher Scientific, Schwerte, Germany); WFDC2/HE4 (DHE400; R&D Systems, Wiesbaden, Germany); SPINT2 (EK0773-CAP; Boster, Pleasanton, USA); PVRL4/NECTIN4 (DNEC40; R&D Systems, Wiesbaden, Germany).

Techniques: Expressing