Journal: Cell Reports Medicine

Article Title: A spatially resolved transcriptome landscape during thyroid cancer progression

doi: 10.1016/j.xcrm.2025.102043

Figure Lengend Snippet: Cell-cell communications in thyroid cancer (A) Bubble plot illustrating the spatial correlations of dysregulated ligand-receptor pairs between PTC, LPTC, or ATC and PT samples in the ST data. (B and C) Bar plots showing fold changes in ligand (B) and receptor (C) expression in PTC, LPTC, and ATC samples compared to PT samples. (D) Spatial feature plots highlighting SERPINE1 (ligand) and PLAUR (receptor) expression in representative samples (PT-2, PTC-2, LPTC-2, and ATC-3). (E and F) Expression levels of SERPINE1 (E) and PLAUR (F) across various cell types. (G) SERPINE1 expression within fibroblasts, with the inset indicating enrichment of fibroblast subpopulations stratified by SERPINE1 expression levels. (H) PLAUR expression within macrophages, with the inset showing enrichment of macrophage subpopulations stratified by PLAUR expression levels. (I) Multiplex immunofluorescence staining for Fib-12 fibroblast markers ( CXCL8 , CXCL1 , and SERPINE1 ) and Mac-11 macrophages ( CXCL12 , STAB1 , and PLAUR ) in PTC, LPTC, and ATC samples. Scale bar: 40 μm. (J) Boxplot comparing SERPINE1 expression in cancer ( n = 58) versus paired NAT ( n = 58) samples from the TCGA thyroid carcinoma (THCA) cohort. (K) Kaplan-Meier survival curve from the TCGA THCA cohort ( n = 510) showing patient survival based on median SERPINE1 expression levels. See also Figure S6 and Tables S5 and .

Article Snippet: Anti-human SERPINE1 antibody, clone 1H4A5 , Proteintech , Cat# 66261-1-Ig; RRID: AB_2881648.

Techniques: Expressing, Multiplex Assay, Immunofluorescence, Staining

Journal: Cell Reports Medicine

Article Title: A spatially resolved transcriptome landscape during thyroid cancer progression

doi: 10.1016/j.xcrm.2025.102043

Figure Lengend Snippet:

Article Snippet: Anti-human SERPINE1 antibody, clone 1H4A5 , Proteintech , Cat# 66261-1-Ig; RRID: AB_2881648.

Techniques: Recombinant, Software

Journal: International Journal of Molecular Sciences

Article Title: Glial-Cell-Line-Derived Neurotrophic Factor Promotes Glioblastoma Cell Migration and Invasion via the SMAD2/3-SERPINE1-Signaling Axis

doi: 10.3390/ijms251810229

Figure Lengend Snippet: SERPINE1 is increased in GBMs. ( A ) GO enrichment analysis of upregulated DEGs in C6 cells treated with GDNF. The top 30 significant GO terms were exhibited according to p -value. ( B ) SERPINE1 mRNA expressions were compared between the GBM and normal tissues from the UALCAN database. ( C ) The overall survival was analyzed to evaluate the association between the mRNA level of SERPINE1 and the outcome of GBM patients by the Oncomine website. ( D , E ) The protein expressions of SERPINE1 in GBM tissues and cells were determined by Western blotting. NB: normal brain; RAs: rat astrocytes; HAs: human astrocytes (vs. NB: **, p < 0.01. vs. RAs: *, p < 0.05. vs. HAs: #, p < 0.05; ##, p < 0.01).

Article Snippet: The concentrations of extracellular SERPINE1 in C6 and U251 cells were evaluated using the rat and human SERPINE1 ELISA Kits (USCNK, Wuhan, China) as previously described [ ].

Techniques: Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Glial-Cell-Line-Derived Neurotrophic Factor Promotes Glioblastoma Cell Migration and Invasion via the SMAD2/3-SERPINE1-Signaling Axis

doi: 10.3390/ijms251810229

Figure Lengend Snippet: GDNF promotes SERPINE1 expressions and secretion in GBM cells. ( A ) Western blotting (left) was used to detect the protein expressions of SERPINE1 in C6 and U251 cells treated with various doses of GDNF (0, 20, 40, 80, 100 ng/mL) for 48 h. The statistical analysis (right) of the left bands was performed. ( B ) The contents of SERPINE1 were tested in C6 and U251 cells after 24 and 48 h of treatment with different concentrations of GDNF using ELISA kits (vs. 0 ng/mL: *, p < 0.05; **, p < 0.01).

Article Snippet: The concentrations of extracellular SERPINE1 in C6 and U251 cells were evaluated using the rat and human SERPINE1 ELISA Kits (USCNK, Wuhan, China) as previously described [ ].

Techniques: Western Blot, Enzyme-linked Immunosorbent Assay

Journal: International Journal of Molecular Sciences

Article Title: Glial-Cell-Line-Derived Neurotrophic Factor Promotes Glioblastoma Cell Migration and Invasion via the SMAD2/3-SERPINE1-Signaling Axis

doi: 10.3390/ijms251810229

Figure Lengend Snippet: SERPINE1 knockdown strikingly suppresses the migration and invasion of GBM cells enhanced by GDNF. ( A , B ) qPCR and Western blotting were applied to assess the knockdown efficiency of SERPINE1 in C6 and U251 cells. ( C , D ) Wound healing assay was performed to examine the effects of SERPINE1 deficiency on cell migration in C6 and U251 cells separately treated with 80 and 20 ng/mL GDNF for 48 h. Bar = 100 µm. ( E , F ) The migratory abilities were detected by transwell migration assay in SERPINE1 silenced C6 and U251 cells treated with 80 and 20 ng/mL GDNF for 48 h, respectively. Bar = 50 µm. ( G ) Transwell invasion assay was conducted to evaluate the influences of SERPINE1 knockdown on cell invasion in C6 and U251 cells after 48 h of separate treatment with 80 and 20 ng/mL GDNF. Bar = 50 µm (*, p < 0.05; **, p < 0.01; ***, p < 0.001).

Article Snippet: The concentrations of extracellular SERPINE1 in C6 and U251 cells were evaluated using the rat and human SERPINE1 ELISA Kits (USCNK, Wuhan, China) as previously described [ ].

Techniques: Knockdown, Migration, Western Blot, Wound Healing Assay, Transwell Migration Assay, Transwell Invasion Assay

Journal: International Journal of Molecular Sciences

Article Title: Glial-Cell-Line-Derived Neurotrophic Factor Promotes Glioblastoma Cell Migration and Invasion via the SMAD2/3-SERPINE1-Signaling Axis

doi: 10.3390/ijms251810229

Figure Lengend Snippet: GDNF upregulates SERPINE1 via SMAD2/3 phosphorylation in GBM cells. ( A , B ) The levels of SMAD2/3 phosphorylation were analyzed in C6 and U251 cells separately treated with 80 and 20 ng/mL GDNF for 0, 1, 2, and 4 h using Western blotting. ( C ) The effects of SMAD2/3 deficiency on SERPINE1 protein expressions were assessed in C6 cells in the presence of 80 ng/mL GDNF for 48 h by Western blotting (*, p < 0.05; **, p < 0.01; ***, p < 0.001).

Article Snippet: The concentrations of extracellular SERPINE1 in C6 and U251 cells were evaluated using the rat and human SERPINE1 ELISA Kits (USCNK, Wuhan, China) as previously described [ ].

Techniques: Phospho-proteomics, Western Blot