Journal: Diabetes

Article Title: Amylase α-2A Autoantibodies

doi: 10.2337/db08-0493

Figure Lengend Snippet: Cloning of human amylase α-2A cDNAs from λTriplEx2 human pancreas cDNA library. Seven clones of human amylase α-2A cDNAs. Their lengths and 5′-ends are shown (A in ATG is designated as 1). The top bar indicates human amylase α-2A cDNA as reported by Wise et al. , and the common regions shared by all seven clones, from codons 299 to 512, are shown in the bottom bar.

Article Snippet: The λTriplEx2 human pancreas large insert cDNA library (HL5517u) and Escherichia coli XL-1 competent cells were obtained from BD Biosciences Clontech (Palo Alto, CA).

Techniques: Clone Assay, cDNA Library Assay

Journal: Diabetes

Article Title: Amylase α-2A Autoantibodies

doi: 10.2337/db08-0493

Figure Lengend Snippet: Prevalence of autoantibody against human AMY-2A in patients with various pancreatic diseases. A : Prevalence of autoantibody against human AMY-2A in patients with AIP ( n = 15), chronic alcoholic pancreatitis (CP, n = 25), pancreatic tumor (PT, n = 25), control subjects from healthy volunteers (C, n = 100), and Hashimoto's thyroiditis (H, n = 47) was examined by ELISA, as described in research design and methods . The data are the mean of triplicate values. The dotted line shows a cutoff value. Fisher's exact test was carried out between AIP and control groups. * P < 0.001. B : Time course of anti-AMY antibody and IgG4 of AIP patients. AIP patient (A.O.), whose IgG was used to screen λTriplEx2 human pancreas cDNA library, was treated with prednisolone ( arrow ). Before and after the treatment, anti-AMY antibody (•-•) and IgG4 (○-○) were measured. In the other AIP patient (T.M.), titer of the anti-AMY antibody (▴-▴) was also measured before and after prednisolone treatment ( arrow ).

Article Snippet: The λTriplEx2 human pancreas large insert cDNA library (HL5517u) and Escherichia coli XL-1 competent cells were obtained from BD Biosciences Clontech (Palo Alto, CA).

Techniques: Enzyme-linked Immunosorbent Assay, cDNA Library Assay